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Rabbit Monoclonal NeuN antibody. Carrier free. Suitable for IHC (PFA fixed), ICC/IF, WB, mIHC, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep samples. Cited in 4 publications.


Images

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (AB209898), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (AB209898), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (AB209898), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (AB209898), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (AB209898), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC (PFA fixed)ICC/IFWBmIHCIHC-FrFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Expected
Tested
Tested
Mouse
Predicted
Expected
Tested
Expected
Tested
Expected
Tested
Rat
Predicted
Expected
Tested
Expected
Expected
Expected
Tested
Cat
Predicted
Expected
Expected
Expected
Expected
Expected
Tested
Common marmoset
Predicted
Expected
Expected
Expected
Expected
Expected
Tested
Dog
Predicted
Expected
Expected
Expected
Expected
Expected
Tested
Goat
Predicted
Expected
Expected
Expected
Expected
Expected
Tested
Sheep
Predicted
Expected
Expected
Expected
Expected
Expected
Tested
Zebrafish
Predicted
Expected
Expected
Expected
Expected
Expected
Tested

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Sheep, Goat, Cat, Dog, Zebrafish, Common marmoset

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Zebrafish

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Common marmoset

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Dog

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Cat

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Goat

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Sheep

Dilution info

-

Notes

See .

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Version

Target data

Function

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

Alternative names

Recommended products

Rabbit Monoclonal NeuN antibody. Carrier free. Suitable for IHC (PFA fixed), ICC/IF, WB, mIHC, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Zebrafish, Common marmoset, Dog, Cat, Goat, Sheep samples. Cited in 4 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR12763

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab209898 is the carrier-free version of Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.

Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Associated diseases and disorders

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

21 product images

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Jianning Lu

    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 staining NeuN in mouse free floating 50 micron lumbar spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS + Triton) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of mouse brain using rabbit anti-NeuN antibody

    IHC-P image of FOX3/NeuN staining on mouse brain (frontal cortex) sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/800 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/800 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of cat cerebellum using rabbit anti-NeuN antibody

    IHC-P image of FOX3/NeuN staining on cat cerebellum sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/1000 dilution).

    Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    IHC-P image of FOX3/NeuN staining on zebrafish spinal cord sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    Clone EPR12763 (ab209898) has been successfully conjugated by Abcam. This image was generated using Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 for protocol details.

    IHC image of Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 staining in formalin fixed paraffin embedded tissue section of normal human cerebellum.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 (1/50) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then counterstained and mounted with SlowFade® Gold Antifade Mountant with DAPI.

    The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is dervied directly from bound Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565. The separate images of Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 and DAPI alone, combined with the merged version of both signals, shows predominant co-localisation of the Alexa Fluor® 647 signal in the nuclei of the cerebellar granule layer.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of sheep brain using rabbit anti-NeuN antibody

    IHC-P image of FOX3/NeuN staining on sheep brain (Frontal cortex) sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/1000 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of mouse cerebellum using rabbit anti-NeuN antibody

    IHC-P image of NeuN (green) and GFAP (red) double staining on mouse cerebellum sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/5000) and Anti-GFAP antibody ab4674 (1/1500) respectively.
    The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were then incubated with Rabbit Monoclonal to NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487) diluted at 1/5000 and Chicken Polyclonal to GFAP (Anti-GFAP antibody ab4674) diluted at 1/1500. The primary antibody was detected using Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097 Goat anti-rabbit IgG conjugated to Alexa Fluor© 488 (1/500) and Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed ab150176 Goat anti-chicken IgY conjugated to Alexa Fluor© 594 (1/500)
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    Clone EPR12763 (ab209898) has been successfully conjugated by Abcam. This image was generated using Anti-NeuN antibody [EPR12763] - Neuronal Marker (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195 for protocol details.

    Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195 staining NeuN in U87-MG cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195 at 1/50 dilution (shown in green) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Alexa Fluor® 594 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    This data was developed using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, the same antibody clone in a different buffer formulation.
    NeuN antibody Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was used with Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: NeuN.
    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.
    For 1 mm brain sections, we recommend a starting dilution of 1:200, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1:400.

  • Western blot - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Western blot - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    This data was developed using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, the same antibody clone in a different buffer formulation. Different batches of Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 were tested on mouse brain lysate at 2.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 46,48 kDa.

    All lanes: Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487)

    Predicted band size: 34 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    An independent comparison of commercially available NeuN clones in IHC-P.

    Competitor A: Leading mouse monoclonal.

    Competitor B: Non-Abcam rabbit monoclonal.

    Sodium citrate was used for antigen retrieval in all 3 samples.

    Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 produces specific staining, equivalent to the leading mouse monoclonal at half the dilution. The non-Abcam mouse monoclonal was less specific as it stained Purkinje cells, which do not express NeuN.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    IHC image of NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487) with VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.

    The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, 0.1µg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866, 1.0µg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

    The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Flow Cytometry (Intracellular) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN flow cytometry staining of U-87 MG cells using rabbit anti-NeuN antibody

    Overlay histogram showing U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells stained with Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (red line).

    The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 1/100 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor®488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 1μg/1x106cells used under the same conditions. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Alexa Fluor® 488 (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195) and Alexa Fluor® 647 (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565) conjugated versions are available for this clone.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunofluorescence staining of SH-SY5Y cells using rabbit anti-NeuN antibody

    Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling NeuN (green) with Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 at 1/300 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200 dilution) was used as the secondary antibody. Counterstained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    ?An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections).

    Competitor A: Leading mouse monoclonal.

    Competitor B: Non-Abcam rabbit monoclonal.

    Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Eva Borger

    Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of mouse brain using rabbit anti-NeuN antibody

    Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 staining NeuN in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with Triton X-100 + 0.4% horse seurm for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of dog cerebellum using rabbit anti-NeuN antibody

    IHC-P image of FOX3/NeuN staining on dog cerebellum sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/500 dilution).

    Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of gliocytoma tissue using rabbit anti-NeuN antibody

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    IHC-P image of FOX3/NeuN staining on rat brain (SVZ) sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    IHC-P image of FOX3/NeuN staining on marmoset cerebellum sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - BSA and Azide free (ab209898)

    NeuN immunohistochemistry staining of goat cerebellum using rabbit anti-NeuN antibody

    IHC-P image of FOX3/NeuN staining on goat cerebellum sections using Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 (1/500 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487).

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