Anti-NeuN antibody [EPR12763] - Neuronal Marker

31 product images

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  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Frozen sections) staining of Mouse hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/mL) dilution (Green).

  Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 green), anti-NeuN (ab177487 red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 grey) on mouse hippocampus.
  Panel B: anti-MAP2 stained on mouse hippocampus.
  Panel C: anti-NeuN stained in neuron of mouse hippocampus.
  Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/mL) dilution.

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  This image is courtesy of a customer review submitted by Jianning Lu

  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  ab177487 staining NeuN in mouse free floating 50 micron lumbar spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with Triton X-100 and blocked with 10% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS + Triton) for 16 hours at 4°C. An Alexa Fluor^® 594-conjugated donkey anti-rabbit IgG polyclonal (1/700) was used as the secondary antibody.

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse brain (frontal cortex) tissue using rabbit Anti-NeuN antibody

  IHC-P image of FOX3/NeuN staining on mouse brain (frontal cortex) sections using ab177487 (1/800 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/800 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-NeuN antibody

  IHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab177487 (1/1000 dilution).

  Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  IHC-P image of FOX3/NeuN staining on zebrafish spinal cord sections using ab177487 (1/500). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of sheep brain tissue using rabbit Anti-NeuN antibody

  IHC-P image of FOX3/NeuN staining on sheep brain (Frontal cortex) sections using ab177487 (1/1000 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

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  Multiplex immunohistochemistry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Fluorescence multiplex immunohistochemical analysis of human cerebellum tissue (formalin-fixed paraffin-embedded section).
  

  Merged staining of Neu-N (ab177487; yellow; Opal™570), anti-beta III Tubulin (Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623; red; Opal™690) and anti-GFAP (Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428; green; Opal™520).

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ kit.

  The section was incubated in three rounds of staining with ab177487 (1/1000 dilution), Anti-beta III Tubulin antibody [EP1569Y] - Neuronal Marker ab52623 (1/200 dilution) and Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 (1/250 dilution); each using a separate fluorescent tyramide signal amplification system.

  Sodium citrate antigen retrieval (pH 6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

  DAPI (blue) was used as a nuclear counter stain.

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  Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Immunofluorescence staining of NeuN using ab177487 in ioGlutamatergic Neurons (Human iPSC-Derived Glutamatergic Neurons, ioGlutamatergic Neurons - Human iPSC-Derived Glutamatergic Neurons ab259259), which were differentiated for 1 day post induction.
  

  The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab177487 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling NeuN with ab177487 at 1/100 dilution (B), SOX1 with Anti-SOX1 antibody [EPR23041-60] ab242125 at 1/100 dilution (C) and Olig2 with Anti-Olig2 antibody [EPR2673] ab109186 at 1/100 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Heat mediated antigen retrieval (Leica ER2, PH9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibodies from the previous round, to avoid any cross-reactivity.
  

  Panel A: merged staining of anti- NeuN (green, Opal™520), anti-SOX1 (red, Opal™570) and anti- Olig2 (yellow, Opal™690).

  Panel B: anti-NeuN stained for neurons.

  Panel C: anti-SOX1 stained on neural progenitors.

  Panel D: anti-Olig2 stained on oligodendrocyte.

  The section was incubated in three rounds of staining: in the order of ab177487, Anti-SOX1 antibody [EPR23041-60] ab242125 and Anti-Olig2 antibody [EPR2673] ab109186 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunocytochemistry/ Immunofluorescence staining of Mouse primary neuron cells using rabbit Anti-NeuN antibody

  Immunocytochemistry/immunofluorescence analysis of Mouse primary neuron cells labelling NeuN with ab177487 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267) at 1/200 dilution and visualised using Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  Confocal image showing mainly nuclear staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-NeuN antibody

  IHC-P image of NeuN (green) and GFAP (red) double staining on mouse cerebellum sections using ab177487 (1/5000) and Anti-GFAP antibody - Astrocyte Marker ab4674 (1/1500) respectively.
  

  The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were then incubated with Rabbit Monoclonal to NeuN (ab177487) diluted at 1/5000 and Chicken Polyclonal to GFAP (Anti-GFAP antibody - Astrocyte Marker ab4674) diluted at 1/1500. The primary antibody was detected using Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097 Goat anti-rabbit IgG conjugated to Alexa Fluor^® 488 (1/500) and Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed ab150176 Goat anti-chicken IgY conjugated to Alexa Fluor^® 594 (1/500)

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  Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Exposure time -
  Lane 1-2: 3 minutes.
  Lane 3-4: 1 minute.
  

  Blocking and dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) at 1/10000 dilution

  Lane 1: Human fetal brain tissue lysate at 10 µg

  Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

  Lane 3: Mouse brain tissue lysate at 10 µg

  Lane 4: Rat brain tissue lysate at 10 µg

  Secondary

  All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

  Predicted band size: 34 kDa

  Observed band size: 46 kDa, 48 kDa

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  Immunohistochemistry (PFA fixed) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN antibody ab177487 was used with Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: NeuN.
  Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.
  For 1 mm brain sections, we recommend a starting dilution of 1:200, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1:400.

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  This image is courtesy of a customer review submitted by Vladimir Milenkovic

  Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-NeuN antibody

  Immunocytochemistry/immunofluorescence analysis of human neurons differentiated from iPSCs labelling NeuN (green) with ab177487 at 1/500 in 0.1% TritonX-100 1% goat serum 1X PBS for 16 hours at 4°C. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Then cells were blocked with 5% serum for 20 minutes at 23°C. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilutionwas used as the secondary antibody. Tuj1 antibody was used to stain neuronal dendrites and axons (red).

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  Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Different batches of ab177487 were tested on mouse brain lysate at 2.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 46,48 kDa.

  All lanes: Western blot - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Predicted band size: 34 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  ​An independent comparison of commercially available NeuN clones in IHC-P.
  

  Competitor A: Leading mouse monoclonal.

  Competitor B: Non-Abcam rabbit monoclonal.

  Sodium citrate was used for antigen retrieval in all 3 samples.

  ab177487 produces specific staining, equivalent to the leading mouse monoclonal at half the dilution. The non-Abcam mouse monoclonal was less specific as it stained Purkinje cells, which do not express NeuN.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  IHC image of NeuN (ab177487) with VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866) staining in formalin fixed paraffin embedded normal human cerebellum tissue section.
  

  The section was dewaxed and then pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked using in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with rabbit monoclonal antibody [EPR12763] to NeuN (ab177487, 0.1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. Endogenous peroxidases were quenched using 1.6% (v/v) hydrogen peroxide in TBS containing 0.025% (v/v) Triton X-100 for 30 minutes at room temperature, with agitation. The secondary antibody, VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866, 1.0μg/ml) was then applied for 1 hour at room temperature in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA before being developed for 10 minutes at room temperature using Steady DAB/Plus (ab103723). The section was then counterstained with hematoxylin and mounted with DPX.

  The negative control (secondary antibody only, no primary) inset shows no staining, demonstrating secondary antibody specificity.

  For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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  Flow Cytometry (Intracellular) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Flow Cytometry (Intracellular) staining of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells using rabbit Anti-NeuN antibody

  Overlay histogram showing U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells stained with ab177487 (red line).

  The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab177487 1/100 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor^®488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 1μg/1x10⁶cells used under the same conditions. Unlabeled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  Alexa Fluor^® 488 (Alexa Fluor® 488 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190195) and Alexa Fluor^® 647 (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565) conjugated versions are available for this clone.

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  Immunocytochemistry/ Immunofluorescence - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunocytochemistry/ Immunofluorescence staining of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells using rabbit Anti-NeuN antibody

  Immunocytochemsitry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling NeuN (green) with ab177487 at 1/300 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/200 dilution) was used as the secondary antibody. Counterstained with DAPI (blue).

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  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  ​An independent comparison of commercially available NeuN clones in IHC-Fr (acetone-fixed mouse dentate gyrus sections).
  

  Competitor A: Leading mouse monoclonal.

  Competitor B: Non-Abcam rabbit monoclonal.

  ab177487 produces intense, specific staining with minimal background, even at half the dilution of competing antibodies.

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  This image is courtesy of a customer review submitted by Eva Borger

  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Frozen sections) staining using rabbit Anti-NeuN antibody

  ab177487 staining NeuN in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with Triton X-100 + 0.4% horse serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 16 hours at 4°C. An Alexa Fluor^® 594-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of dog cerebellum tissue using rabbit Anti-NeuN antibody

  IHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab177487 (1/500 dilution).

  Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-NeuN antibody

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling NeuN with ab177487 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with Hematoxylin.

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  IHC-P image of FOX3/NeuN staining on rat brain (SVZ) sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  IHC-P image of FOX3/NeuN staining on marmoset cerebellum sections using ab177487 (1/2000). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/2000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250).

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  This image is courtesy of a customer review submitted by Carl Hobbs, Kings's College London, United Kingdom.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of goat cerebellum tissue using rabbit Anti-NeuN antibody

  IHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab177487 (1/500 dilution). Sections were de-paraffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 minutes at 21°C. ab177487 was diluted 1/500 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin (1/250 dilution).

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  Multiplex immunohistochemistry - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Chromogenic multiplex immunohistochemical staining of FFPE normal human cerebellum tissue. ab177487, anti-NeuN DAB chromogen. Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428, anti-GFAP purple chromogen and Anti-Iba1 antibody [EPR16588] ab178846, anti- Iba1 teal chromogen plus haematoxylin counterstain.
  Chromogenic immunostaining was performed on a Roche Ventana Discovery Ultra instrument. The section was deparaffinised and incubated with CC1 solution for 24min 100°C. Following this with 3 rounds of staining in the order of ab177487 (1/600), Anti-Iba1 antibody [EPR16588] ab178846 (1/4000) Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 (1/1000). Between rounds of staining, antibody denaturation was conducted using Ultra CC2 solution for 8min at 100°C to avoid cross reactivity. Signal was developed with anti-rabbit HQ followed by anti-HQ HRP coupled with Chromomap DAB kit, Discovery purple or Discovery teal chromogens and haematoxylin II counterstain.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human cerebellum tissue using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of formalin fixed paraffin embedded human cerebellum labelling NeuN with ab177487 at a dilution of 1/500. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an ChromoMap DAB (RUO) IHC Detection Kit with anti rabbit HQ and anti HQ HRP. Heat mediated antigen retrieval was conducted for 24 min with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5. ab177487 was incubated at 37°C for 16 min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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  Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN Immunohistochemistry (Frozen sections) staining of Rat hippocampus (fresh frozen) using rabbit Anti-NeuN antibody

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

  Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (ab177487, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on rat hippocampus.
  
  Panel B: anti-MAP2 stained on rat hippocampus.
  
  Panel C: anti-NeuN stained in neuron of rat hippocampus.
  
  Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
  
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Secondary antibody control: Secondary antibody is Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

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  Flow Cytometry (Intracellular) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  Flow cytometry overlay histogram showing Neuro-2a cells stained with ab177487 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab177487) (1x 10⁶in 100µl at 5.0µg/ml (1/444)) for 30min at 22°C.

  The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

  Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

  Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  This antibody gave a positive signal in Neuro-2a Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

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  IHC-tissue clearing - Anti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487)

  NeuN IHC-tissue clearing staining of whole mouse brain using rabbit Anti-NeuN antibody

  Anti-NeuN ab177487 was used with Tissue clearing kit – CUBIC (Tissue Clearing Kit – CUBIC ab316246) and 3D Tissue Staining Kit – CUBIC (3D Tissue Staining Kit – CUBIC ab316248) to penetrate stain and clear a whole mouse brain.

  White: nuclear staining
  Red: NeuN.

  Learn more about tissue clearing kits reagents and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample. For a whole mouse brain we recommend starting with 10 ug of ab177487 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). Additive A was used during the staining process.

  The sample was imaged using a light-sheet microscope.