Anti-NeuN antibody [EPR21902]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (AB236869)

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID : 8813082; PMID : 1483388). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (AB236869)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID : 8813082; PMID : 1483388; PMID : 26085943). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody [EPR21902] (AB236869)

Immunohistochemical analysis of paraffin-embedded human retina tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in ganglion cells of human retina (PMID : 8813082; PMID : 1483388). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-NeuN antibody [EPR21902] (AB236869)

NeuN was immunoprecipitated from 0.35 mg human fetal brain lysate with ab236869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human fetal brain lysate 10 μg (Input).
Lane 2 : ab236869 IP in human fetal brain lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab236869 in human fetal brain lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 8 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1483388).

All lanes:

Immunoprecipitation - Anti-NeuN antibody [EPR21902] (ab236869)

Predicted band size: 34 kDa

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• WB

Supplier Data

Western blot - Anti-NeuN antibody [EPR21902] (AB236869)

Exposure times : Lanes 1 and 3-4 : 15 seconds; Lane 2 : 92 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1483388; PMID : 26085943).

All lanes:

Western blot - Anti-NeuN antibody [EPR21902] (ab236869) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human brain lysate at 20 µg

Lane 3:

Human cerebellum lysate at 20 µg

Lane 4:

Human hippocampus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 46 kDa,48 kDa

false

• WB

Lab

Western blot - Anti-NeuN antibody [EPR21902] (AB236869)

Western blot : Rabbit Monoclonal[EPR21902] to NeuN ab236869 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in RBFOX3 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-NeuN antibody [EPR21902] (ab236869) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-NeuN antibody [EPR21902] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/neun-antibody-epr21902-bsa-and-azide-free-ab239345'>ab239345</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

RBFOX3 knockout A549 at 20 µg

Lane 3:

U-2 OS at 20 µg

Lane 4:

Raji at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 40 kDa

false