AB128886

Anti-NeuN antibody - Neuronal Marker

Name: Anti-NeuN antibody - Neuronal Marker (ab128886) | Abcam
Brand: Abcam Limited
SKU: AB128886
Price: 405 USD
Availability: InStock
Rating: 5 (14 reviews)

Lab Essentials
What is this?

(14 Reviews)

(54 Publications)

Rabbit Polyclonal NeuN antibody. Neuron marker. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 54 publications.

View Alternative Names

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

IHC-P

AbReview33756****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

IHC-P image of FOX3/NeuN staining on rat brain sections of Cortex (upper image) and lateral ventricle - SVZ (lower image) using ab128886 (1 : 3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1 : 3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

IHC-P image of FOX3/NeuN staining on rat brain sections using ab128886 (1 : 3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1 : 3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

IHC-P image of FOX3/NeuN staining on mouse cerebellum sections using ab128886 (1 : 2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1 : 2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.

This image is courtesy of Carl Hobbs, King's College London, United Kingdom

Lab

Western blot - Anti-NeuN antibody - Neuronal Marker (AB128886)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab128886 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886) at 5 µg/mL

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Mouse brain lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat anti Rabbit IR680 at 1/10000 dilution

Predicted band size: 34 kDa

Observed band size: 15 kDa,48 kDa,50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.

Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).

See full target information NeuN

style

left-column

Publications (54)

Recent publications for all applications. Explore the full list and refine your search

NPJ Parkinson's disease 11:122 PubMed40348747

2025

An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ.

Applications

Unspecified application

Species

Unspecified reactive species

P Stalder,T Serdiuk,D Ghosh,Y Fleischmann,N Ait-Bouziad,J-P Quast,L Malinovska,A Ouared,A Davranche,W Haenseler,C Boudou,E Tsika,J Stöhr,R Melki,R Riek,N de Souza,P Picotti

Nutrients 15: PubMed37892455

2023

Botanical Mixture Containing Nitric Oxide Metabolite Enhances Neural Plasticity to Improve Cognitive Impairment in a Vascular Dementia Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorong Zhang,Seung-Bum Yang,Lin Cheng,Koo Ho,Min-Sun Kim

International journal of molecular sciences 24: PubMed37834141

2023

Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Liao,Qing Zhu,Han Huang

Neuroscience 512:85-98 PubMed36549605

2022

Amyloid Deposition and Dendritic Complexity of Corticocortical Projection Cells in Five Familial Alzheimer's Disease Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Celine Plachez,Vassiliy Tsytsarev,Shuxin Zhao,Reha S Erzurumlu

Proceedings of the National Academy of Sciences of the United States of America 119:e2209427119 PubMed36227915

2022

Aberrant cortical spine dynamics after concussive injury are reversed by integrated stress response inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Elma S Frias,Mahmood S Hoseini,Karen Krukowski,Maria Serena Paladini,Katherine Grue,Gonzalo Ureta,Kira D A Rienecker,Peter Walter,Michael P Stryker,Susanna Rosi

Frontiers in pharmacology 13:971243 PubMed36120298

2022

Effects of Tibetan medicine metacinnabar (β-HgS) combined with imipramine or sertraline on depression-like symptoms in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Qiao,Cen Li,Ming Zhang,Xingfang Zhang,Lixin Wei,Keshen Cao,Xiaoyuan Zhang,Hongtao Bi,Tingting Gao

Cellular and molecular neurobiology 43:1385-1399 PubMed35933637

2022

Fetal Brain Damage in Human Fetuses with Congenital Cytomegalovirus Infection: Histological Features and Viral Tropism.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Piccirilli,Liliana Gabrielli,Maria Paola Bonasoni,Angela Chiereghin,Gabriele Turello,Eva Caterina Borgatti,Giuliana Simonazzi,Silvia Felici,Marta Leone,Nunzio Cosimo Mario Salfi,Donatella Santini,Tiziana Lazzarotto

Neural regeneration research 18:176-182 PubMed35799539

2022

Diffusion tensor imaging reveals brain structure changes in dogs after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Bin Liu,De-Gang Yang,Jun Li,Chuan Qin,Xin Zhang,Jun Liu,Da-Peng Li,Jian-Jun Li

International journal of stem cells 15:258-269 PubMed35769054

2022

Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS).

Applications

Unspecified application

Species

Unspecified reactive species

Kyu-Ree Kang,C-Yoon Kim,Jin Kim,Bokyeong Ryu,Seul-Gi Lee,Jieun Baek,Ye-Ji Kim,Jin-Moo Lee,Yootmo Lee,Sun-Ok Choi,Dong Ho Woo,Il Hwan Park,Hyung Min Chung

International journal of molecular medicine 50: PubMed35730627

2022

Silencing of circular RNA‑ZYG11B exerts a neuroprotective effect against retinal neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Ma,Mu-Di Yao,Xiao-Yan Han,Ze-Hui Shi,Biao Yan,Jian-Ling Du

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NeuN antibody - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB128886)

Western blot - Anti-NeuN antibody - Neuronal Marker (AB128886)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (54)

Product promise