JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB134014

Anti-NeuN antibody - Neuronal Marker

2

(9 Reviews)

|

(34 Publications)

Chicken Polyclonal NeuN antibody. Neuron marker. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Mouse Rbfox3 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RNA binding protein fox-1 homolog 3, Fox-1 homolog C, Neuronal nuclei antigen, NeuN antigen, RBFOX3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB134014)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB134014)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse CA1 hippocampus tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB134014)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (AB134014)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adult mouse cerebral cortex tissue labelling NeuN (red) with ab134014. Tissue was fixed with 4% paraformaldehyde. Green - rabbit anti-Beta-Tubulin 3.

Western blot - Anti-NeuN antibody - Neuronal Marker (AB134014)
  • WB

Ap

Western blot - Anti-NeuN antibody - Neuronal Marker (AB134014)

All lanes:

Western blot - Anti-NeuN antibody - Neuronal Marker (ab134014) at 1/1000 dilution

Lane 1:

Mouse Brain at 20 µg

Lane 2:

Rat Brain at 20 µg

Predicted band size: 34 kDa

Observed band size: 42 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Rbfox3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8BIF2

Specificity

We recommend ab177487 as an alternative for IHC-P and WB.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>NeuN is a nuclear antigen and ab134014 is a chicken antibody, which has a slightly higher molecular weight compared to IgG. We recommend performing heat mediated antigen retrieval with Citrate buffer pH 6.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>NeuN is a nuclear antigen and ab134014 is a chicken antibody, which has a slightly higher molecular weight compared to IgG. We recommend performing heat mediated antigen retrieval with Citrate buffer pH 6.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>NeuN is a nuclear antigen and ab134014 is a chicken antibody, which has a slightly higher molecular weight compared to IgG. We recommend performing heat mediated antigen retrieval with Citrate buffer pH 6.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab134014 was affinity-purified from the IgY fraction prepared from eggs laid by immunised hens.
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 0.9% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuN also known as Fox-3 or Rbfox3 serves as a vital neuronal marker and has a molecular weight of about 46-50 kDa. This nuclear protein is commonly found in neuronal cells in the central nervous system but it does not appear in glial cells. Researchers use NeuN staining as an effective tool to identify neurons in various brain regions due to its reliable expression pattern in mature neurons. The presence of NeuN can be detected using methods such as NeuN IHC (immunohistochemistry) and NeuN Western blot aiding in cellular and tissue analysis.
Biological function summary

NeuN plays an essential role in the regulation of RNA-binding influencing the splicing and stability of transcripts. It forms part of the complex network responsible for managing neuron-specific gene expression. By interacting with other RNA-binding proteins NeuN contributes to the fine-tuning needed for proper neuronal development and function. As a result NeuN helps maintain the health and activity of nerve cells allowing them to perform complex neurological tasks.

Pathways

NeuN has a significant role in neuronal differentiation and plasticity pathways. NeuN coordinates with elements of the Notch signaling pathway which is important for guiding cell fate decisions in the nervous system. It also interacts with related proteins like Fox proteins involved in splicing regulation. These pathways ensure neurons develop differentiate and function correctly within the nervous system's precise architecture.

Disruptions in NeuN expression link closely to neurodegenerative diseases such as Parkinson's disease and Amyotrophic lateral sclerosis (ALS). Abnormal NeuN expression correlates with neuron loss and dysfunction in these conditions with connectivity to other affected proteins like alpha-synuclein in Parkinson's disease. In ALS abnormal NeuN-staining patterns indicate neuronal cell death serving as a potential marker for disease progression. Studying these connections improves our understanding of disease mechanisms and aids in the development of targeted therapies.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
See full target information NeuN

Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:170 PubMed40581646

2025

Genetically-engineered Salmonella typhimurium expressing FGF21 promotes neurological recovery in ischemic stroke via FGFR1/AMPK/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongchen Xu,Min Wen,Bingwa Lebohang Anesu,Xijun Chen,Yuhao Chen,Wenqi Qian,Chenguang Yang,Jin Hai Zheng,Yinan Zhou,Haoqi Ni,Kunlin Jin,Qichuan Zhuge,Su Yang

Scientific reports 14:25305 PubMed39455718

2024

An innovative electrical neurostimulation approach to mimic reflexive urination control in spinal cord injury models.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Guoxian Deng,Xianping Li,Lingxuan Yin,Chunhui Yuan,Wei Shao,Yuangui Chen,Jiwei Yao,Junan Yan

Journal of extracellular vesicles 13:e12439 PubMed38647111

2024

Neuropathogenic role of astrocyte-derived extracellular vesicles in HIV-associated neurocognitive disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Divya T Chemparathy,Sudipta Ray,Chase Ochs,Natasha Ferguson,Dinesh Y Gawande,Shashank M Dravid,Shannon Callen,Susmita Sil,Shilpa Buch

International journal of nanomedicine 19:2265-2284 PubMed38476273

2024

Chitosan-Rapamycin Carbon Dots Alleviate Glaucomatous Retinal Injury by Inducing Autophagy to Promote M2 Microglial Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Jiaxin Dong,Mengxian Du,Xinna Liu,Shiqi Zhang,Di Zhang,Wanyun Qin,Xikun Xu,Xianghui Li,Ruidong Su,Leyi Qiu,Baoqiang Li,Huiping Yuan

Molecular brain 17:5 PubMed38317261

2024

Outer layer of Vb neurons in medial entorhinal cortex project to hippocampal dentate gyrus in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Yamamoto,Jun Yokose,Kritika Ramesh,Takashi Kitamura,Sachie K Ogawa

GeroScience 46:2503-2519 PubMed37989825

2023

Immune proteins C1q and CD47 may contribute to aberrant microglia-mediated synapse loss in the aging monkey brain that is associated with cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah A DeVries,Bryce Conner,Christina Dimovasili,Tara L Moore,Maria Medalla,Farzad Mortazavi,Douglas L Rosene

Science immunology 8:eadg2979 PubMed37862432

2023

Long 3'UTRs predispose neurons to inflammation by promoting immunostimulatory double-stranded RNA formation.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler J Dorrity,Heegwon Shin,Kenenni A Wiegand,Justin Aruda,Michael Closser,Emily Jung,Jake A Gertie,Amanda Leone,Rachel Polfer,Bruce Culbertson,Lisa Yu,Christine Wu,Takamasa Ito,Yuefeng Huang,Anna-Lena Steckelberg,Hynek Wichterle,Hachung Chung

The Journal of clinical investigation 133: PubMed36719741

2023

Neuron-oligodendrocyte potassium shuttling at nodes of Ranvier protects against inflammatory demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Kapell,Luca Fazio,Julia Dyckow,Sophia Schwarz,Andrés Cruz-Herranz,Christina Mayer,Joaquin Campos,Elisa D'Este,Wiebke Möbius,Christian Cordano,Anne-Katrin Pröbstel,Marjan Gharagozloo,Amel Zulji,Venu Narayanan Naik,Anna Delank,Manuela Cerina,Thomas Müntefering,Celia Lerma-Martin,Jana K Sonner,Jung Hyung Sin,Paul Disse,Nicole Rychlik,Khalida Sabeur,Manideep Chavali,Rajneesh Srivastava,Matthias Heidenreich,Kathryn C Fitzgerald,Guiscard Seebohm,Christine Stadelmann,Bernhard Hemmer,Michael Platten,Thomas J Jentsch,Maren Engelhardt,Thomas Budde,Klaus-Armin Nave,Peter A Calabresi,Manuel A Friese,Ari J Green,Claudio Acuna,David H Rowitch,Sven G Meuth,Lucas Schirmer

iScience 26:106152 PubMed36879821

2023

Moesin is an effector of tau-induced actin overstabilization, cell cycle activation, and neurotoxicity in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Beckmann,Paulino Ramirez,Maria Gamez,Elias Gonzalez,Jasmine De Mange,Kevin F Bieniek,William J Ray,Bess Frost

Neurotoxicology 94:98-107 PubMed36402194

2022

Receptor protein tyrosine phosphatase β/ζ regulates loss of neurogenesis in the mouse hippocampus following adolescent acute ethanol exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Milagros Galán-Llario,María Rodríguez-Zapata,Esther Gramage,Marta Vicente-Rodríguez,Teresa Fontán-Baselga,María Carmen Ovejero-Benito,Carmen Pérez-García,Javier Carrasco,Marco Moreno-Herradón,Julio Sevillano,María Pilar Ramos-Álvarez,José María Zapico,Beatriz de Pascual-Teresa,Ana Ramos,Gonzalo Herradón
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com