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AB197020

Anti-Neuraminidase antibody [EPR15712]

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(5 Publications)

Rabbit Recombinant Monoclonal Neuraminidase antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

NANH, NEU1, Sialidase-1, Acetylneuraminyl hydrolase, G9 sialidase, Lysosomal sialidase, N-acetyl-alpha-neuraminidase 1

3 Images
Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)
  • WB

Lab

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)

Western blot : Anti-Neuraminidase antibody [EPR15712] ab197020 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40-48 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in NEU1 knockout A549 UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/2000 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

Western blot - Human NEU1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-neu1-knockout-a549-cell-line-ab301095'>ab301095</a>) at 20 µg

Lane 3:

HeLa UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 40-48 kDa,37 kDa

false

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)
  • WB

Supplier Data

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)

Blocking and diluting buffer was 5% NFDM /TBST

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

true

Exposure time: 3min

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)
  • WB

Supplier Data

Western blot - Anti-Neuraminidase antibody [EPR15712] (AB197020)

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (ab197020) at 1/10000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

true

Exposure time: 3min

  • Carrier free

    Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15712

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/140", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuraminidase sometimes called sialidase functions as an enzyme that cleaves sialic acid residues from glycoproteins and glycolipids. This process is essential for the maturation and release of progeny viruses from infected cells. Enzymatically active neuraminidase proteins weigh about 60 kilodaltons and localize on the surface of influenza viruses. Researchers identify its expression on the viral envelope playing a pivotal role in the viral life cycle. Neuraminidase activity is an important target for drug development against influenza.
Biological function summary

Neuraminidase significantly impacts viral virulence and host cell infection. It is part of the functional machinery required for viral particle detachment from the host cell membrane facilitating the spread of infection. Neuraminidase works alongside other viral proteins including hemagglutinin which attaches the virus to the host cell. The neuraminidase function of trimming sialic acids is critical in the ongoing interaction between virus and host.

Pathways

The influenza lifecycle heavily involves neuraminidase-mediated sialic acid cleavage. This enzyme contributes to pathways by enabling the efficient release of new viral particles enhancing the infectivity of the virus. Neuraminidase operates in concert with hemagglutinin which initially binds the virus to host cells by attaching to the sialic acid residues. This interplay is vital in viral replication pathways which propagate the infection cycle.

Influenza directly relates to neuraminidase as it is a primary means by which the virus spreads within the host. Neuraminidase inhibitors are a class of antiviral drugs that target this protein important for treating and managing influenza infections. Besides influenza research links neuraminidase activity to bacterial infections with some bacterial neuraminidases implicated in the pathogenesis of diseases like pneumonia. The relationship to hemagglutinin in viral influenza highlights the significance of neuraminidase as a target in therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.
See full target information NEU1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Annals of human genetics 89:126-131 PubMed40165614

2025

Novel CTSA Variant Identified in a Thai Family With Late-Infantile Galactosialidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukana Ngiwsara,Dhachdanai Dhachpramuk,Phannee Sawangareetrakul,Sherry Vongphit,Punchama Pacharn,Jisnuson Svasti,Nithiwat Vatanavicharn

Biological research 57:68 PubMed39342328

2024

Identification and functional characteristics of CHD1L gene variants implicated in human Müllerian duct anomalies.

Applications

Unspecified application

Species

Unspecified reactive species

Shuya Chen,Yali Fan,Yujun Sun,Shenghui Li,Zhi Zheng,Chunfang Chu,Lin Li,Chenghong Yin

Molecular biology reports 50:3035-3043 PubMed36662453

2023

Ureaplasma parvum infection induces inflammatory changes in vaginal epithelial cells independent of sialidase.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuel Amabebe,Lauren S Richardson,Giovana Fernanda Cosi Bento,Enkhtuya Radnaa,Talar Kechichian,Ramkumar Menon,Dilly O C Anumba

Virology 569:44-55 PubMed35255298

2022

Functional antibody-dependent cell mediated cytotoxicity (ADCC) responses to vaccine and circulating influenza strains following vaccination.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemin Chen,He-Ying Sun,Chun Yi Lee,Christina A Rostad,Jessica Trost,Rodrigo B Abreu,Michael A Carlock,Jason R Wilson,Shane Gansebom,Ted M Ross,David A Steinhauer,Evan J Anderson,Larry J Anderson

Frontiers in pharmacology 12:805499 PubMed35002739

2021

Elevated N-Glycosylation Contributes to the Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Revealed by Membrane Proteomic and Glycoproteomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Zeng,Shanshan Zheng,Yonghong Mao,Shisheng Wang,Yi Zhong,Wei Cao,Tao Su,Meng Gong,Jingqiu Cheng,Yong Zhang,Hao Yang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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