Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free

• WB

Lab

Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (AB251229)

This data was developed using ab197020, the same antibody clone in a different buffer formulation.

Western blot : Anti-Neuraminidase antibody [EPR15712] ab197020 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 40-48 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in NEU1 knockout A549 UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (<a href='/en-us/products/primary-antibodies/neuraminidase-antibody-epr15712-ab197020'>ab197020</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

Western blot - Human NEU1 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-neu1-knockout-a549-cell-line-ab301095'>ab301095</a>) at 20 µg

Lane 3:

HeLa UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 40-48 kDa,37 kDa

false

• WB

Supplier Data

Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (AB251229)

This data was developed using ab197020, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (<a href='/en-us/products/primary-antibodies/neuraminidase-antibody-epr15712-ab197020'>ab197020</a>) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Neuraminidase antibody [EPR15712] - BSA and Azide free (AB251229)

This data was developed using ab197020, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Neuraminidase antibody [EPR15712] (<a href='/en-us/products/primary-antibodies/neuraminidase-antibody-epr15712-ab197020'>ab197020</a>) at 1/10000 dilution

All lanes:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

true

Exposure time: 3min