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AB122400

Anti-NEURL1B antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal NEURL1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NEURL1B aa 150 to C-terminus.

View Alternative Names

NEURL3, NEURL1B, E3 ubiquitin-protein ligase NEURL1B, Neuralized-2, Neuralized-like protein 1B, Neuralized-like protein 3, RING-type E3 ubiquitin transferase NEURL1B, NEUR2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NEURL1B antibody (AB122400)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NEURL1B antibody (AB122400)

Immunofluorescent staining of Human cell line U-251 MG shows positivity in vesicles. Recommended concentration of ab122400 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEURL1B antibody (AB122400)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NEURL1B antibody (AB122400)

ab122400, at a 1/225 dilution, staining NEURL1B in glandular cells of paraffin-embedded Human rectum tissue by immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NEURL1B aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

A8MQ27

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NEURL1B also known as neuralized E3 ubiquitin protein ligase 1B functions as an E3 ubiquitin ligase. This protein plays a role in tagging other proteins for degradation by the proteasome a process critical for maintaining cellular protein balance. NEURL1B has a molecular mass of approximately 70 kDa and is expressed markedly in neural tissues. It sometimes appears in other tissues but its expression is not as pronounced as in the nervous system.
Biological function summary

The protein acts as a regulator of the Notch signaling pathway which is essential for cell differentiation and proliferation. NEURL1B does not work alone; instead it often operates as a part of a larger protein complex that includes other ligases and substrates. Through this complex formation NEURL1B modifies signal transduction processes influencing various cellular functions.

Pathways

NEURL1B is tightly linked to the Notch signaling and Wnt/β-catenin pathways. These signaling routes are instrumental in development and oncogenesis. Within these pathways NEURL1B interacts with the Notch receptor and can inhibit its activity by promoting degradation. It also connects to other proteins such as DLL1 within the Notch pathway contributing to the modulation of cell fate decisions.

NEURL1B has associations with cancer and neurodevelopmental disorders. Dysregulation of the Notch signaling pathway where NEURL1B operates often correlates with tumorigenesis in specific cancers. Furthermore alterations in NEURL1B function are implicated in disorders like autism spectrum disorder potentially impacting neuronal connectivity and synapse formation. The protein's interaction with other signaling molecules such as the Notch receptor suggests its significance in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase involved in regulation of the Notch pathway through influencing the stability and activity of several Notch ligands.
See full target information NEURL1B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32764283

2020

Inclusion Formation and Toxicity of the ALS Protein RGNEF and Its Association with the Microtubule Network.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja E Di Gregorio,Kathryn Volkening,Michael J Strong,Martin L Duennwald
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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