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AB199299

Anti-Neuro D4 antibody - C-terminal

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Rabbit Polyclonal Neuro D4 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DPF1 aa 150 to C-terminus.

View Alternative Names

BAF45B, NEUD4, DPF1, Zinc finger protein neuro-d4, BRG1-associated factor 45B

2 Images
Western blot - Anti-Neuro D4 antibody - C-terminal (AB199299)
  • WB

Supplier Data

Western blot - Anti-Neuro D4 antibody - C-terminal (AB199299)

All lanes:

Western blot - Anti-Neuro D4 antibody - C-terminal (ab199299) at 1/500 dilution

All lanes:

U87 cell lysate

Predicted band size: 43 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuro D4 antibody - C-terminal (AB199299)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuro D4 antibody - C-terminal (AB199299)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal brain tissue labeling Neuro D4 with ab199299 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DPF1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92782

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Reactivity data

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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuro D4 also known as NEUROD4 operates as a basic helix-loop-helix (bHLH) transcription factor. It functions by binding to DNA sequences to regulate gene expression. This protein plays a critical role in the differentiation of neuronal cells. Researchers have identified NEUROD4 in tissues with notable expression in the nervous system. The protein has a molecular mass of approximately 36 kDa which supports its role in cellular processes.
Biological function summary

NEUROD4 influences the development of neural tissues by promoting neural lineage differentiation. It acts as part of a transcriptional regulation complex essential for bending genetic programs towards neurogenesis. Adaptations in NEUROD4 function impact neurogenesis which is essential for constructing a complex nervous system throughout developmental stages. This transcription factor orchestrates various cellular components to achieve specific neural fate decisions.

Pathways

NEUROD4 has a significant influence on the Notch and Wnt signaling pathways which are key to understanding its role in neural differentiation. It interacts with proteins like E47 another bHLH transcription factor to impact the signaling cascades that encourage precursor cells to mature into neurons. These pathways help in toggling between cell proliferation and differentiation enabling NEUROD4 to support balanced neural development.

NEUROD4 is connected to neurodevelopmental disorders notably with autism spectrum disorder due to its pivotal role in the formation of neural networks. Abnormalities in its expression or function might result in improper neuronal differentiation leading to developmental complications. Research also links it to retinopathy disorders where proteins like RAX which interact with NEUROD4 play a role in retinal development and maintenance leading to vision issues if dysfunction occurs.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
See full target information DPF1
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