AB31979

Anti-Neurocan antibody [650.24]

(4 Reviews)

(4 Publications)

Mouse Monoclonal Neurocan antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Tissue preparation containing Ncan protein.

View Alternative Names

Cspg3, Neurocan core protein, 245 kDa early postnatal core glycoprotein, Chondroitin sulfate proteoglycan 3

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (AB31979)

ICC/IF

AbReview25905****

Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (AB31979)

ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.

Image courtesy of an anonymous Abreview.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

650.24

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Tissue preparation containing Ncan protein. The exact immunogen used to generate this antibody is proprietary information.

P55067

Specificity

Specific for Neurocan core protein; also reacts with neurocan C fragment

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurocan also known as CSPG3 is a chondroitin sulfate proteoglycan with a core protein mass around 245 kDa. It is expressed mostly in the central nervous system. Neurocan plays a mechanical role in modulation of cell adhesion and migration interacting with other extracellular matrix components like tenascin-C and hyaluronic acid. This interaction helps regulate how neurons and glial cells move and connect during development and after injury.

Biological function summary

Neurocan functions significantly in the neural extracellular matrix influencing neural plasticity and maintaining brain homeostasis. It is often found as part of a proteoglycan complex interacting with other matrix molecules. Its molecular structure allows it to bind and influence growth factors impacting neurogenesis and synaptic maintenance. The presence of neurocan is especially significant during periods of neural development and remodeling serving as a regulatory element in these processes.

Pathways

Neurocan plays a part in the regulation of nervous system development and neural repair mechanisms. The protein actively participates in the MAPK signaling pathway affecting cellular responses like proliferation and differentiation. Neurocan relates to proteins like fibronectin and integrins within these pathways contributing to structure and signaling that affect cellular environments and neuron network formations.

Neurocan holds associations with conditions like schizophrenia and neurotrauma. Altered expression or mutations within the neurocan gene can contribute to the disrupted neural connectivity seen in schizophrenia. Furthermore neurocan interacts with proteins such as aggrecan and versican in response to neural injuries forming glial scars that can inhibit proper neural repair and recovery processes. Understanding the role of neurocan in these diseases is essential in developing therapeutic strategies targeting neural plasticity and repair mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.

See full target information Ncan

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3917 PubMed37400444

2023

Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey.

Applications

Unspecified application

Species

Unspecified reactive species

Jingkuan Wei,Shaoxing Dai,Yaping Yan,Shulin Li,Pengpeng Yang,Ran Zhu,Tianzhuang Huang,Xi Li,Yanchao Duan,Zhengbo Wang,Weizhi Ji,Wei Si

Frontiers in cellular neuroscience 15:569424 PubMed33815059

2021

TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation the Smurf2/ALK5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Su,Shaoping Fan,Lingqiong Zhang,Hui Qi

CNS neuroscience & therapeutics 26:940-951 PubMed32449258

2020

Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xianpeng Huang,Chenggui Wang,Xiaopeng Zhou,Jingkai Wang,Kaishun Xia,Biao Yang,Zhe Gong,Liwei Ying,Chao Yu,Kesi Shi,Jiawei Shu,Feng Cheng,Bin Han,Chengzhen Liang,Fangcai Li,Qixin Chen

Frontiers in cellular neuroscience 10:241 PubMed27807407

2016

Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats.

Applications

ICC/IF, WB

Species

Rat, Rat

Gang Li,Yang Cao,Feifei Shen,Yangsong Wang,Liangjie Bai,Weidong Guo,Yunlong Bi,Gang Lv,Zhongkai Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurocan antibody [650.24]

Immunocytochemistry/ Immunofluorescence - Anti-Neurocan antibody [650.24] (AB31979)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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