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AB125021

Anti-Neurocan antibody [EPR6397]

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(2 Publications)

Rabbit Recombinant Monoclonal Neurocan antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CSPG3, NEUR, NCAN, Neurocan core protein, Chondroitin sulfate proteoglycan 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurocan antibody [EPR6397] (AB125021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurocan antibody [EPR6397] (AB125021)

ab125021 at 1/250 dilution staining Neurocan in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Neurocan antibody [EPR6397] (AB125021)
  • WB

Unknown

Western blot - Anti-Neurocan antibody [EPR6397] (AB125021)

All lanes:

Western blot - Anti-Neurocan antibody [EPR6397] (ab125021) at 1/10000 dilution

Lane 1:

Human hippocampus lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 143 kDa

Observed band size: 130 kDa,270 kDa

false

OI-RD Scanning - Anti-Neurocan antibody [EPR6397] (AB125021)
  • OI-RD Scanning

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OI-RD Scanning - Anti-Neurocan antibody [EPR6397] (AB125021)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6397

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurocan also known as CSPG3 is a chondroitin sulfate proteoglycan with a core protein mass around 245 kDa. It is expressed mostly in the central nervous system. Neurocan plays a mechanical role in modulation of cell adhesion and migration interacting with other extracellular matrix components like tenascin-C and hyaluronic acid. This interaction helps regulate how neurons and glial cells move and connect during development and after injury.
Biological function summary

Neurocan functions significantly in the neural extracellular matrix influencing neural plasticity and maintaining brain homeostasis. It is often found as part of a proteoglycan complex interacting with other matrix molecules. Its molecular structure allows it to bind and influence growth factors impacting neurogenesis and synaptic maintenance. The presence of neurocan is especially significant during periods of neural development and remodeling serving as a regulatory element in these processes.

Pathways

Neurocan plays a part in the regulation of nervous system development and neural repair mechanisms. The protein actively participates in the MAPK signaling pathway affecting cellular responses like proliferation and differentiation. Neurocan relates to proteins like fibronectin and integrins within these pathways contributing to structure and signaling that affect cellular environments and neuron network formations.

Neurocan holds associations with conditions like schizophrenia and neurotrauma. Altered expression or mutations within the neurocan gene can contribute to the disrupted neural connectivity seen in schizophrenia. Furthermore neurocan interacts with proteins such as aggrecan and versican in response to neural injuries forming glial scars that can inhibit proper neural repair and recovery processes. Understanding the role of neurocan in these diseases is essential in developing therapeutic strategies targeting neural plasticity and repair mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
See full target information NCAN
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 15:569424 PubMed33815059

2021

TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation the Smurf2/ALK5 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Su,Shaoping Fan,Lingqiong Zhang,Hui Qi

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:15603-17 PubMed24068827

2013

Chondroitin sulfate proteoglycans potently inhibit invasion and serve as a central organizer of the brain tumor microenvironment.

Applications

Unspecified application

Species

Human

Daniel J Silver,Florian A Siebzehnrubl,Michela J Schildts,Anthony T Yachnis,George M Smith,Amy A Smith,Bjorn Scheffler,Brent A Reynolds,Jerry Silver,Dennis A Steindler
View all publications
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Product promise

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