Mouse Monoclonal NeuroD1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 59 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NEUROD1 aa 200-300.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113759.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704)")
![Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--western-blot-img92718.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704)")
![Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--flow-cytometry-img103069.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (AB60704)")
Publications
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- iScience 26:1078312023KMT2D suppresses Sonic hedgehog-driven medulloblastoma progression and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReeti Mayur Sanghrajka,Richard Koche,Hector Medrano,Salsabiel El Nagar,Daniel N Stephen,Zhimin Lao,N Sumru Bayin,Kai Ge,Alexandra L JoynerPubMed 37822508
- Cells 12:2023MicroRNA-375 Is Induced during Astrocyte-to-Neuron Reprogramming and Promotes Survival of Reprogrammed Neurons when Overexpressed.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanyu Chen,Ivan Sokirniy,Xin Wang,Mei Jiang,Natalie Mseis-Jackson,Christine Williams,Kristopher Mayes,Na Jiang,Brendan Puls,Quansheng Du,Yang Shi,Hedong LiPubMed 37681934
- Journal of clinical biochemistry and nutrition 73:191-1972023Induction of -amplified neuroblastoma differentiation through NMYC suppression using PPAR-γ antagonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukako Nakao-Ise,Takumi Narita,Shingo Miyamoto,Motoki Watanabe,Takuji Tanaka,Yoshihiro Sowa,Yosuke Iizumi,Mitsuharu Masuda,Gen Fujii,Yasuko Hirai,Toshimasa Nakao,Hideki Takakura,Michihiro MutohPubMed 37970556
- Antioxidants (Basel, Switzerland) 12:2023Protective Effects of Early Caffeine Administration in Hyperoxia-Induced Neurotoxicity in the Juvenile Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Heise,Thomas Schmitz,Christoph Bührer,Stefanie EndesfelderPubMed 36829854
- Signal transduction and targeted therapy 7:3462022Single-cell transcriptomic profiling reveals the tumor heterogeneity of small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanhua Tian,Qingqing Li,Zhenlin Yang,Shu Zhang,Jiachen Xu,Zhijie Wang,Hua Bai,Jianchun Duan,Bo Zheng,Wen Li,Yueli Cui,Xin Wang,Rui Wan,Kailun Fei,Jia Zhong,Shugeng Gao,Jie He,Carl M Gay,Jianjun Zhang,Jie Wang,Fuchou TangPubMed 36195615
- Translational oncology 25:1015382022Prognostic significance of YAP1 expression and its association with neuroendocrine markers in resected pulmonary large cell neuroendocrine carcinoma (LCNEC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujie Sun,Jinyao Zhang,Jiyan Dong,Li Liu,Xue Li,Puyuan Xing,Jianming Ying,Yiqun Che,Junling Li,Lin YangPubMed 36103754
- eLife 11:2022Temporal analysis of enhancers during mouse cerebellar development reveals dynamic and novel regulatory functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel Ramirez,Yuliya Badayeva,Joanna Yeung,Joshua Wu,Ayasha Abdalla-Wyse,Erin Yang,Brett Trost,Stephen W Scherer,Daniel GoldowitzPubMed 35942939
- Scientific reports 12:125132022Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoão Brás,Daniel Henriques,Ricardo Moreira,Magda M Santana,Rita Silva-Pedrosa,Diana Adão,Sandra Braz,Ana Rita Álvaro,Luís Pereira de Almeida,Liliana S MendonçaPubMed 35869235
- Biomedicines 10:2022BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during Aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Li,Hongmei Liu,Dongfang Jiang,Keyan Yang,Jiaqi Shen,Haiping Feng,Sijia Wang,Yuxin Zhang,Yun Wang,Tie-Shan TangPubMed 35625884
- Advanced genetics (Hoboken, N.J.) 3:21000602022Single-Cell Transcriptome Identifies Drug-Resistance Signature and Immunosuppressive Microenvironment in Metastatic Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang,Haiping Zhang,Lele Zhang,Dianke Li,Mengfan Qi,Liping Zhang,Huansha Yu,Di Wang,Gening Jiang,Xujun Wang,Xianmin Zhu,Peng ZhangPubMed 36618022
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human NEUROD1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13562
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Sheep, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
Alternative names
BHLHA3, NEUROD, NEUROD1, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3
Recommended products
Mouse Monoclonal NeuroD1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 59 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NEUROD1 aa 200-300.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human NEUROD1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13562
- Carrier free
- Yes
- Clone number
- 3H8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
- Biological function summary
NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.
- Pathways
NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.
- Associated diseases and disorders
Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113759.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
Immunohistochemical analysis of formaldehyde fixed human cerebellum sections incubated with ab60704 for 20 minutes at 25°C in a concentration of 1/400. The blocking step was performed with 3% H2O2 for 10 minutes at 25°C. The secondary antibody used was a polyclonal goat anti-mouse/rabbit HRP conjugate, used undiluted.
This image was generated using the ascites version of the product.
![Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--western-blot-img92718.jpg "Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)")
Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
All lanes: IMR-32 (Human Caucasian neuroblastoma) whole cell lysate at 50 µg
SecondaryAll lanes: Goat Anti-Mouse IgG HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 150 kDa, 40 kDa
![Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--flow-cytometry-img103069.jpg "Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)")
Flow Cytometry - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
Overlay histogram showing SHSY-5Y cells stained with ab60704 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60704, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-3h8-bsa-and-azide-free-ab60704--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74304.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [3H8] - BSA and Azide free (ab60704)
ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
This image was generated using the ascites version of the product.
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