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Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit monoclonal antibody detecting NeuroD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications



Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail
  • Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail
  • Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail
  • Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBFlow Cyt (Intra)IHC-PICCIHC-Fr
Human
Tested
Tested
Tested
Expected
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).

Alternative names

Recommended products

Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit monoclonal antibody detecting NeuroD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 20 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20766
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of NeuroD1?


Anti-NeuroD1 [EPR20766] (ab213725) specifically detects a band for NeuroD1 (UniProt: Q13562) at a molecular weight of 40kDa.

Trusted by the scientific community


Anti-NeuroD1 [EPR20766] (ab213725) was first used in a scientific publication in 2017 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR20766] also available for your convenience:
ab213725, Carrier free - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free ab226489



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.

Biological function summary

NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.

Pathways

NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.

Associated diseases and disorders

Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Blocking/dilution buffer: 5% NFDM/TBST

    Exposure times.

    Lane 1: 10 seconds

    Lane 2: 30 seconds

    All lanes: Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1/1000 dilution

    Lane 1: Y79 (human retinoblastoma cell line) whole cell lysate at 20 µg

    Lane 2: Mouse P3 (postnatal day 3) retina lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

    Observed band size: 39 kDa

  • Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Blocking/dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1000 Cells

    All lanes: Rat retina tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 40 kDa

    Observed band size: 39 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1,000 dilution

    Lane 1: Y79 whole cell lysate 10 μg (Input).
    Lane 2: ab213725 IP in Y79 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213725 in Y79 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Developed using the ECL technique.

    Predicted band size: 40 kDa

    Observed band size: 39 kDa

  • Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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