Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit monoclonal antibody detecting NeuroD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122506.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (AB213725)")
![Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--western-blot-img92953.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725)")
![Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--western-blot-img158715.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NeuroD1 antibody [EPR20766] (AB213725)")
![Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (AB213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunoprecipitation-img5913.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (AB213725)")
Publications
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- iScience 26:1084432023Chronic AMPK inactivation slows SHH medulloblastoma progression by inhibiting mTORC1 signaling and depleting tumor stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Shiloh Malawsky,Taylor Dismuke,Hedi Liu,Ethan Castellino,Jay Brenman,Biplab Dasgupta,Andrey Tikunov,Timothy R GershonPubMed 38094249
- International journal of molecular sciences 24:2023Skin Wound following Irradiation Aggravates Radiation-Induced Brain Injury in a Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMang Xiao,Xianghong Li,Li Wang,Bin Lin,Min Zhai,Lisa Hull,Alex Zizzo,Wanchang Cui,Juliann G KiangPubMed 37445879
- EBioMedicine 90:1045312023New AAV tools fail to detect Neurod1-mediated neuronal conversion of Müller glia and astrocytes in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Xie,Jing Zhou,Lei-Lei Wang,Chun-Li Zhang,Bo ChenPubMed 36947961
- Metabolites 13:2023Expression Silencing of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 Conferred Its Role in Pancreatic β-Cell Physiology and Insulin Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Saeed,Abdul Khader Mohammed,Sarra E Saleh,Khaled M Aboshanab,Mohammad M Aboulwafa,Jalal TaneeraPubMed 36837926
- Neurochemical research 48:996-10082022GPR137 Inhibits Cell Proliferation and Promotes Neuronal Differentiation in the Neuro2a Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKensuke Iwasa,Anzu Yamagishi,Shinji Yamamoto,Chikara Haruta,Kei Maruyama,Keisuke YoshikawaPubMed 36436172
- ESMO open 7:1006312022Clinical and prognostic implications of CD47 and PD-L1 expression in surgically resected small-cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesC Lang,A Lantos,Z Megyesfalvi,F Egger,M A Hoda,B Mosleh,T Klikovits,F Oberndorfer,G Timelthaler,B Ferencz,J Fillinger,A Schwendenwein,A S Querner,K Boettiger,F Renyi-Vamos,K Hoetzenecker,V Laszlo,K Schelch,B DomePubMed 36399951
- Development (Cambridge, England) 149:2022Dentate gyrus morphogenesis is regulated by β-catenin function in hem-derived fimbrial glia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpan Parichha,Debarpita Datta,Varun Suresh,Mallika Chatterjee,Michael J Holtzman,Shubha TolePubMed 36196585
- Cancer science 113:3932-39462022ASCL1 regulates super-enhancer-associated miRNAs to define molecular subtypes of small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuko Miyakawa,Naoya Miyashita,Masafumi Horie,Yasuhiro Terasaki,Hidenori Tanaka,Hirokazu Urushiyama,Kensuke Fukuda,Yugo Okabe,Takashi Ishii,Naomi Kuwahara,Hiroshi I Suzuki,Takahide Nagase,Akira SaitoPubMed 35789143
- Journal of neurochemistry 163:8-252022Deletion of serine racemase reverses neuronal insulin signaling inhibition by amyloid-β oligomers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhou,Zhiwen Zhang,Yuanhong Yang,Fei Liao,Piansi Zhou,Yan Wang,He Zhang,Haiyan Jiang,Tahereh Alinejad,Ge Shan,Shengzhou WuPubMed 35839294
- Biology 11:2022Reduced Retinoic Acid Receptor Beta (Rarβ) Affects Pancreatic β-Cell Physiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnila Khalique,Abdul Khader Mohammed,Nujood Mohammed Al-Khadran,Mutaz Al Gharaibeh,Eman Abu-Gharbieh,Waseem El-Huneidi,Nabil Sulaiman,Jalal TaneeraPubMed 36101450
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | IHC-P | ICC | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
| Mouse | Expected | Tested | Expected | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Expected | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
Alternative names
BHLHA3, NEUROD, NEUROD1, Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3
Recommended products
Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit monoclonal antibody detecting NeuroD1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR20766
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-NeuroD1 antibody [EPR20766] (ab213725) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of NeuroD1?
Anti-NeuroD1 [EPR20766] (ab213725) specifically detects a band for NeuroD1 (UniProt: Q13562) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-NeuroD1 [EPR20766] (ab213725) was first used in a scientific publication in 2017 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR20766] also available for your convenience:
ab213725, Carrier free - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free ab226489
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
- Biological function summary
NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.
- Pathways
NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.
- Associated diseases and disorders
Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122506.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--western-blot-img92953.jpg "Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Blocking/dilution buffer: 5% NFDM/TBST
Exposure times.
Lane 1: 10 seconds
Lane 2: 30 seconds
All lanes: Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1/1000 dilution
Lane 1: Y79 (human retinoblastoma cell line) whole cell lysate at 20 µg
Lane 2: Mouse P3 (postnatal day 3) retina lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 39 kDa
![Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--western-blot-img158715.jpg "Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Blocking/dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-NeuroD1 antibody [EPR20766] (ab213725) at 1000 Cells
All lanes: Rat retina tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 39 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--immunoprecipitation-img5913.jpg "Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)
NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1,000 dilution
Lane 1: Y79 whole cell lysate 10 μg (Input).
Lane 2: ab213725 IP in Y79 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213725 in Y79 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
All lanes: Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--flow-cytometry-intracellular-img26795.jpg "Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725)
Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-ab213725--flow-cytometry-intracellular-img26795.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] (ab213725)")
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