Rabbit Recombinant Monoclonal NeuroD1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122506.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489)")
![Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunoprecipitation-img5913.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489)")
![Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--flow-cytometry-intracellular-img26795.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (AB226489)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Expected |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Rat | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
Alternative names
Neurogenic differentiation factor 1, NeuroD, NeuroD1, Class A basic helix-loop-helix protein 3, bHLHa3, NEUROD, NEUROD1, BHLHA3
Recommended products
Rabbit Recombinant Monoclonal NeuroD1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR20766
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226489 is the carrier-free version of Anti-NeuroD1 antibody [EPR20766] ab213725.
The Human species recommendation is based on the WB results. We do not guarantee IHC-P for Human.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NeuroD1 also referred to as Neurogenic Differentiation 1 is a basic helix-loop-helix transcription factor with a molecular mass of approximately 40 kDa. This protein is expressed mainly in the central nervous system as well as in the pancreas. NeuroD1 plays a role in activating transcription by binding to DNA sequences in specific target genes. This process involves dimerization with E proteins facilitating its function as a transcriptional activator.
- Biological function summary
NeuroD1 influences neural development and differentiation. It is vital in converting undifferentiated neural precursors into mature neurons ensuring proper brain formation. NeuroD1 forms part of a transcriptional complex with other neural transcription factors which coordinates the expression of genes needed for neuron specification. Apart from neurogenesis it regulates insulin gene expression in pancreatic beta cells linking neural functions to endocrine regulation.
- Pathways
NeuroD1 functions in the neurogenesis pathway and interacts closely with the Notch signaling pathway. It plays a role by influencing the expression of genes essential for neuronal lineage commitment. In the insulin signaling pathway NeuroD1 cooperates with other transcription factors like Pdx1 and MafA managing the expression of insulin and other important beta-cell genes. Interactions with proteins like E47 increase its regulatory effects on these pathways.
- Associated diseases and disorders
Defects in NeuroD1 function connect to conditions such as diabetes mellitus and medulloblastoma. In diabetes mellitus NeuroD1 mutations can disturb insulin gene regulation impacting glucose metabolism severely. The connection with Pdx1 further magnifies its involvement in pancreatic beta-cell function. In medulloblastoma aberrant expression or mutations in NeuroD1 affect neuronal differentiation leading to tumor development. NeuroD1 influences cellular pathways interacting with proteins such as Sox9 which contributes to oncogenic processes in neural tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling NeuroD1 with Anti-NeuroD1 antibody [EPR20766] ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the mouse hippocampus dentate gyrus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuroD1 antibody [EPR20766] ab213725).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img122506.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling NeuroD1 with Anti-NeuroD1 antibody [EPR20766] ab213725 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on subgranular zone of the rat hippocampus (PMID: 19701197, PMID: 25825708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuroD1 antibody [EPR20766] ab213725).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--immunoprecipitation-img5913.jpg "Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)")
Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)
NeuroD1 was immunoprecipitated from 0.35 mg of Y79 (human retinoblastoma cell line) whole cell lysate with Anti-NeuroD1 antibody [EPR20766] ab213725 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-NeuroD1 antibody [EPR20766] ab213725 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Y79 whole cell lysate 10 μg (Input).
Lane 2: Anti-NeuroD1 antibody [EPR20766] ab213725 IP in Y79 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NeuroD1 antibody [EPR20766] ab213725 in Y79 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuroD1 antibody [EPR20766] ab213725).
All lanes: Immunoprecipitation - Anti-NeuroD1 antibody [EPR20766] (Anti-NeuroD1 antibody [EPR20766] ab213725)
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489), expandable thumbnail](https://content.abcam.com/products/images/neurod1-antibody-epr20766-bsa-and-azide-free-ab226489--flow-cytometry-intracellular-img26795.jpg "Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)")
Flow Cytometry (Intracellular) - Anti-NeuroD1 antibody [EPR20766] - BSA and Azide free (ab226489)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Y79 (human retinoblastoma cell line) cell line labeling NeuroD1 with Anti-NeuroD1 antibody [EPR20766] ab213725 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype control details (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NeuroD1 antibody [EPR20766] ab213725).
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