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AB109406

Anti-NeuroD2 antibody [EPR5135]

5

(3 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal NeuroD2 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

BHLHA1, NDRF, NEUROD2, Neurogenic differentiation factor 2, NeuroD2, Class A basic helix-loop-helix protein 1, NeuroD-related factor, bHLHa1

3 Images
Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Unknown

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human hippocampus lysate at 10 µg

Lane 3:

Human fetal brain lysates at 10 µg

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Lab

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)
  • WB

Lab

Western blot - Anti-NeuroD2 antibody [EPR5135] (AB109406)

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (ab109406) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysates at 15 µg

Lane 2:

Rat cerebellum lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 48 kDa

false

  • Carrier free

    Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5135

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD2 also known as Neuronal Differentiation 2 is a transcription factor involved in neurogenesis. It weighs approximately 40 kDa. This protein is part of the basic helix-loop-helix (bHLH) family which regulates gene expression. NeuroD2 is expressed mainly in the nervous system particularly in the developing brain and certain adult brain regions. Its expression pattern suggests an important role in neural development and maintenance.
Biological function summary

NeuroD2 influences neuronal differentiation and survival. It guides precursor cells toward a neuronal fate influencing neurogenesis and impacting brain structure. NeuroD2 does not form large protein complexes but interacts closely with other bHLH transcription factors. These interactions help regulate the transcription of genes necessary for the development and maturation of neurons.

Pathways

Transcription factors such as NeuroD2 play key roles in the neurogenesis pathway and the calcium signaling pathway. It operates in tandem with other proteins like NeuroD1 to effectively guide neural precursor cells toward differentiation into mature neurons. Through these pathways NeuroD2 helps regulate genes that are important for brain development and function.

Abnormal NeuroD2 expression relates to neurological conditions like autism spectrum disorders and certain types of epilepsy. Mutations or dysregulation in NeuroD2 can affect associated proteins like REST (RE1-Silencing Transcription factor) which plays a role in neuronal gene silencing. Understanding these connections provides insights into how NeuroD2 influences neural-related diseases and offers potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity).
See full target information NEUROD2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:2588 PubMed32444594

2020

KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Lipinski,Rafael Muñoz-Viana,Beatriz Del Blanco,Angel Marquez-Galera,Juan Medrano-Relinque,José M Caramés,Andrzej A Szczepankiewicz,Jordi Fernandez-Albert,Carmen M Navarrón,Roman Olivares,Grzegorz M Wilczyński,Santiago Canals,Jose P Lopez-Atalaya,Angel Barco

Scientific reports 9:19697 PubMed31873146

2019

Terminal neuron localization to the upper cortical plate is controlled by the transcription factor NEUROD2.

Applications

Unspecified application

Species

Unspecified reactive species

Gizem Guzelsoy,Cansu Akkaya,Dila Atak,Cory D Dunn,Alkan Kabakcioglu,Nurhan Ozlu,Gulayse Ince-Dunn

Science advances 5:eaax0080 PubMed31681843

2019

Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria A Hahn,Seung-Gi Jin,Arthur X Li,Jiancheng Liu,Zhijun Huang,Xiwei Wu,Byung-Wook Kim,Jennifer Johnson,Adrienne-Denise V Bilbao,Shu Tao,Jacob A Yim,Yuman Fong,Sandra Goebbels,Markus H Schwab,Qiang Lu,Gerd P Pfeifer

Nature communications 10:4112 PubMed31511512

2019

In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene.

Applications

Unspecified application

Species

Unspecified reactive species

Cyril J Peter,Atsushi Saito,Yuto Hasegawa,Yuya Tanaka,Mohika Nagpal,Gabriel Perez,Emily Alway,Sergio Espeso-Gil,Tariq Fayyad,Chana Ratner,Aslihan Dincer,Achla Gupta,Lakshmi Devi,John G Pappas,François M Lalonde,John A Butman,Joan C Han,Schahram Akbarian,Atsushi Kamiya

International journal of molecular sciences 20: PubMed30934833

2019

Mechanistic Insights in NeuroD Potentiation of Mineralocorticoid Receptor Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa T C M van Weert,Jacobus C Buurstede,Hetty C M Sips,Isabel M Mol,Tanvi Puri,Ruth Damsteegt,Benno Roozendaal,R Angela Sarabdjitsingh,Onno C Meijer

eNeuro 4: PubMed28303257

2017

NEUROD2 Regulates Expression and Store-Operated Calcium Entry in Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Gokhan Guner,Gizem Guzelsoy,Fatma Sadife Isleyen,Gulcan Semra Sahin,Cansu Akkaya,Efil Bayam,Eser Ilgin Kotan,Alkan Kabakcioglu,Gulayse Ince-Dunn

BMC genomics 16:681 PubMed26341353

2015

Genome-wide target analysis of NEUROD2 provides new insights into regulation of cortical projection neuron migration and differentiation.

Applications

Unspecified application

Species

Mouse

Efil Bayam,Gulcan Semra Sahin,Gizem Guzelsoy,Gokhan Guner,Alkan Kabakcioglu,Gulayse Ince-Dunn

The Journal of biological chemistry 287:27396-406 PubMed22736761

2012

Contribution of microRNA-1275 to Claudin11 protein suppression via a polycomb-mediated silencing mechanism in human glioma stem-like cells.

Applications

WB

Species

Human

Keisuke Katsushima,Keiko Shinjo,Atsushi Natsume,Fumiharu Ohka,Makiko Fujii,Hirotaka Osada,Yoshitaka Sekido,Yutaka Kondo
View all publications
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