AB242385

Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NeuroD2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

BHLHA1, NDRF, NEUROD2, Neurogenic differentiation factor 2, NeuroD2, Class A basic helix-loop-helix protein 1, NeuroD-related factor, bHLHa1

2 Images

Unknown

Western blot - Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free (AB242385)

This data was developed using ab109406, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (<a href='/en-us/products/primary-antibodies/neurod2-antibody-epr5135-ab109406'>ab109406</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human hippocampus lysate at 10 µg

Lane 3:

Human fetal brain lysates at 10 µg

Predicted band size: 41 kDa

Observed band size: 48 kDa

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Lab

Western blot - Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free (AB242385)

This data was developed using ab109406, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NeuroD2 antibody [EPR5135] (<a href='/en-us/products/primary-antibodies/neurod2-antibody-epr5135-ab109406'>ab109406</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 41 kDa

Observed band size: 48 kDa

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Unconjugated

Anti-NeuroD2 antibody [EPR5135]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5135

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab242385 is the carrier-free version of ab109406.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NeuroD2 also known as Neuronal Differentiation 2 is a transcription factor involved in neurogenesis. It weighs approximately 40 kDa. This protein is part of the basic helix-loop-helix (bHLH) family which regulates gene expression. NeuroD2 is expressed mainly in the nervous system particularly in the developing brain and certain adult brain regions. Its expression pattern suggests an important role in neural development and maintenance.

Biological function summary

NeuroD2 influences neuronal differentiation and survival. It guides precursor cells toward a neuronal fate influencing neurogenesis and impacting brain structure. NeuroD2 does not form large protein complexes but interacts closely with other bHLH transcription factors. These interactions help regulate the transcription of genes necessary for the development and maturation of neurons.

Pathways

Transcription factors such as NeuroD2 play key roles in the neurogenesis pathway and the calcium signaling pathway. It operates in tandem with other proteins like NeuroD1 to effectively guide neural precursor cells toward differentiation into mature neurons. Through these pathways NeuroD2 helps regulate genes that are important for brain development and function.

Abnormal NeuroD2 expression relates to neurological conditions like autism spectrum disorders and certain types of epilepsy. Mutations or dysregulation in NeuroD2 can affect associated proteins like REST (RE1-Silencing Transcription factor) which plays a role in neuronal gene silencing. Understanding these connections provides insights into how NeuroD2 influences neural-related diseases and offers potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator implicated in neuronal determination. Mediates calcium-dependent transcription activation by binding to E box-containing promoter. Critical factor essential for the repression of the genetic program for neuronal differentiation; prevents the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Induces transcription of ZEB1, which in turn represses neuronal differentiation by down-regulating REST expression. Plays a role in the establishment and maturation of thalamocortical connections; involved in the segregation of thalamic afferents into distinct barrel domains within layer VI of the somatosensory cortex. Involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Associates with chromatin to the DPYSL3 E box-containing promoter (By similarity).

See full target information NEUROD2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free

Western blot - Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free (AB242385)

Western blot - Anti-NeuroD2 antibody [EPR5135] - BSA and Azide free (AB242385)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise