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AB279391

Anti-NEUROD4 antibody [OTI1B7]

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Mouse Monoclonal NEUROD4 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEUROD4.

View Alternative Names

ATH3, ATOH3, BHLHA4, NEUROD4, Neurogenic differentiation factor 4, NeuroD4, Class A basic helix-loop-helix protein 4, Protein atonal homolog 3, bHLHa4, ATH-3, Atoh3

1 Images
Western blot - Anti-NEUROD4 antibody [OTI1B7] (AB279391)
  • WB

Supplier Data

Western blot - Anti-NEUROD4 antibody [OTI1B7] (AB279391)

All lanes:

Western blot - Anti-NEUROD4 antibody [OTI1B7] (ab279391) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 (BALB/c mouse embryonic fibroblast SV40 transformed cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B7

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEUROD4.

Q9HD90

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NEUROD4 gene encodes a neuronal differentiation factor known as Neurogenic Differentiation 4 or bHLH (basic helix-loop-helix family member E33). This protein plays a critical role in neurogenesis and is closely related to the NeuroD family. NeuroD4 weighs approximately 42 kDa and expresses mainly in the developing central nervous system particularly in regions associated with sensory neuron development.
Biological function summary

Neurogenic Differentiation 4 functions as a transcription factor regulating gene expression during neural development. It participates in forming transcriptional complexes with other bHLH proteins facilitating neuronal differentiation and proliferation. By binding to E-box sequences in the DNA NEUROD4 influences the transcription of genes important for neuronal identity and precursory cell maturation.

Pathways

The NeuroD4 protein integrates into neurodevelopmental pathways including the Notch signaling and the Wnt signaling pathways. These pathways are pivotal for the control of cell fate decisions and proliferation in the nervous system. NeuroD4 interacts with other proteins within these pathways such as Notch intracellular domain proteins and TCF/LEF transcription factors further influencing the regulation of specific gene sets important for neural development.

Neurogenic Differentiation 4 associates with neurodevelopmental disorders like autism spectrum disorder and neuronal structure abnormalities such as retinal degenerations. Mutations or dysregulation of NEUROD4 can disrupt normal neural differentiation processes leading to these conditions. Connections to related proteins in these disorders include interactions with Onecut transcription factors relevant in developing retinal and other neural tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably acts as a transcriptional activator. Mediates neuronal differentiation. Required for the regulation of amacrine cell fate specification in the retina (By similarity).
See full target information NEUROD4
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