Rabbit Polyclonal NFASC antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Common marmoset samples. Cited in 24 publications.
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- Journal of cell science 136:2023Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNevena Stajković,Yuanyuan Liu,Aleksandra Arsić,Ning Meng,Hang Lyu,Nan Zhang,Dirk Grimm,Holger Lerche,Ivana Nikić-SpiegelPubMed 37288813
- The Journal of biological chemistry 298:1022722022Crystal structure of Ankyrin-G in complex with a fragment of Neurofascin reveals binding mechanisms required for integrity of the axon initial segment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiping He,Wenli Jiang,Jianchao Li,Chao WangPubMed 35850303
- Pharmacogenomics and personalized medicine 15:377-3922022Identification of and as Two Novel Hub Genes in Endometriosis Using Integrated Bioinformatic Analysis and Experimental Verification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei Chen,Mengyun Yao,Tao Fang,Chaoshuang Ye,Yongjiang Du,Yang Jin,Ruijin WuPubMed 35496348
- Microscopy research and technique 85:2679-26912022Glucose increases the length and spacing of the lattice structure of the axon initial segment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiming Wang,Meiling Guan,Yan Zhang,Karl Zhanghao,Peng XiPubMed 35411984
- Cerebral cortex (New York, N.Y. : 1991) 31:5024-50412021Oligodendrocyte Secreted Factors Shape Hippocampal GABAergic Neuron Transcriptome and Physiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Mazuir,Louis Richevaux,Merie Nassar,Noémie Robil,Pierre de la Grange,Catherine Lubetzki,Desdemona Fricker,Nathalie Sol-FoulonPubMed 34023893
- Frontiers in cellular neuroscience 15:5766092021Deletion of the Thrombin Proteolytic Site in Neurofascin 155 Causes Disruption of Nodal and Paranodal Organization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDipankar J Dutta,R Douglas FieldsPubMed 33815060
- Glia 68:1794-18092020Loss-of-function of EBP50 is a new cause of hereditary peripheral neuropathy: EBP50 functions in peripheral nerve system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyun Jee Song,Deepak Prasad Gupta,Md Habibur Rahman,Hwan Tae Park,Imad Al Ghouleh,Alessandro Bisello,Maan-Gee Lee,Jae-Yong Park,Hyun Ho Park,Jin Hyun Jun,Ki Wha Chung,Byung-Ok Choi,Kyoungho SukPubMed 32077526
- Brain communications 2:fcaa0122020Intraepidermal nerve fibre density as biomarker in Charcot-Marie-Tooth disease type 1A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeate Hartmannsberger,Kathrin Doppler,Julia Stauber,Beate Schlotter-Weigel,Peter Young,Michael W Sereda,Claudia SommerPubMed 32954280
- Parkinsonism & related disorders 63:66-722019Neurofascin (NFASC) gene mutation causes autosomal recessive ataxia with demyelinating neuropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdoardo Monfrini,Letizia Straniero,Sara Bonato,Giacomo Monzio Compagnoni,Andreina Bordoni,Robertino Dilena,Paola Rinchetti,Rosamaria Silipigni,Dario Ronchi,Stefania Corti,Giacomo P Comi,Nereo Bresolin,Stefano Duga,Alessio Di FonzoPubMed 30850329
- The Journal of clinical investigation 128:2252-22652018Mutant ataxin1 disrupts cerebellar development in spinocerebellar ataxia type 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChandrakanth Reddy Edamakanti et. al.PubMed 29533923
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-FoFr | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested |
| Mouse | Tested | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
| Common marmoset | Predicted | Predicted | Expected | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Common marmoset, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.
Alternative names
KIAA0756, NFASC, Neurofascin
Recommended products
Rabbit Polyclonal NFASC antibody. Suitable for WB, IHC-FoFr, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Common marmoset samples. Cited in 24 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The ab31457 antibody would be able to bind to the full length canonical sequence Isoform 1 and also to Isoforms 5, Isoform 6, Isoform 9 and Isoform 13.
The antibody would not be able to detect Isoforms 2, 3, 4, 7, 8, 10, 11 or Isoform 12. All of these Isoforms are missing amino acids 1035-1203.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neurofascin also known as NFASC is a cell adhesion molecule with significant roles in the nervous system. It has various isoforms with Neurofascin-186 and Neurofascin-155 being the most studied. The molecular mass of Neurofascin is approximately 135 kDa. This protein is expressed in the nervous system particularly at nodes of Ranvier and paranodal regions as well as in the initial segment of axons. Neurofascin contributes to organizing and maintaining the axonal domains essential for neural signal transmission.
- Biological function summary
This neural protein plays an integral role in developing and securing the nervous system's structural organization. Neurofascin helps form the nodes of Ranvier which are critical for saltatory conduction enhancing rapid and efficient nerve impulse transmission. It also interacts with other proteins such as Ankyrin G and Contactin to maintain the stability and function of neuronal domains. Through these interactions Neurofascin facilitates the proper conduction and coordination of electrical signals along neurons.
- Pathways
Neurofascin is essential for neural signal transmission and maintenance pathways. It prominently participates in the axon guidance and myelination pathways working alongside proteins like Caspr (Contactin-associated protein) and Ankyrin B. These pathways ensure the growth and maintenance of myelin sheaths supporting nerve insulation and efficient signal relay. Neurofascin's interaction with these proteins reinforces the nodes of Ranvier's organization promoting stable and rapid signal conduction in the peripheral and central nervous systems.
- Associated diseases and disorders
Research links Neurofascin with autoimmune neuropathies like Guillain-Barré syndrome and multiple sclerosis. These conditions involve the immune-mediated destruction of myelin leading to severe motor and sensory deficits. Neurofascin acts as a target in some autoimmune responses where antibodies may mistakenly attack the protein disrupting neural conduction. Connections between Neurofascin and other proteins like Contactin and Ankyrin G highlight the complex interactions that can lead to pathological states when disrupted. Developing therapies targeting Neurofascin-related pathways holds potential for mitigating these disorders' impacts on neuronal functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Neurofascin antibody (ab31457)
In Rat, Neurofascin has three observed isoforms , isoform 1 (138 kDa) which is known as Nfasc186, isoform 2 (133 kDa) and isoform 3 (132 kDa) which are both known as Nfasc155. These proteins are highly glycosylated and their observed molecular weight in Western blot is 186 kDa and 155 kDa respectively.
Mouse Neurofascin has a predicted molecular weight of 138 kDa (Swiss-Prot data) however the observed bands at 186 kDa and 155 kDa are thought to correspond to the glycosylated isoforms 1, 2 and 3 as observed in Rat (Sherman DL et al, 2005,PubMed:16337912)All lanes: Western blot - Anti-Neurofascin antibody (ab31457) at 1 µg/mL
All lanes: Brain(Mouse)Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 120 kDa, 155 kDa, 186 kDa, 55 kDa
This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neurofascin antibody (ab31457)
Immunohistochemistical detection of Neurofascin using antibody ab31457 on PFA perfusion-fixed rat brain sections. Primary antibody ab31457 was used at 1/1000 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. Some cytoplasmic staining was observed in granular neurons and neuronal processes (arrows on the figure on the right). The lefthand figure shows the hippocampal granular cell staining (objective X20), the righthand figure shows this at higher magnification. This staining is consistent with published observation of cytoplasmic localisation of Neurofascin in the cytoplasm of hippocampal cells and their axon initial segment (Burkarth
Immunocytochemistry/ Immunofluorescence - Anti-Neurofascin antibody (ab31457)
ab31457 staining Neurofascin in Rat Primary Neurons DIV14 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31457 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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