AB184377

Anti-Neurofascin antibody [EPR19003]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal NFASC antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

KIAA0756, NFASC, Neurofascin

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurofascin with ab184377 at 1/5000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm and membrane staining on myenteric nerve plexus and negative on epithelium cells and smooth muscle cells of mouse colon.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on rat cerebrum is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofascin with ab184377 at 1/5000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on mouse cerebrum is observed [PMID : 23198679].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Neurofascin antibody [EPR19003] (AB184377)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight and expression profile observed are consistent with the literatures (PMID : 23160985; PMID : 9562181).

The 75 kDa band represents one of the isoforms annotated in UniProt.

All lanes:

Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

Lane 1:

Human brain lysate at 20 µg

Lane 2:

Human cerebellum lysate at 20 µg

Lane 3:

Human lung lysate at 20 µg

Lane 4:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 150 kDa

Observed band size: 125 kDa,155 kDa,186 kDa,75 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Neurofascin antibody [EPR19003] (AB184377)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight and expression profile observed are consistent with the literatures (PMID : 23160985; PMID : 9562181).

The 75 kDa band represents one of the isoforms annotated in UniProt.

All lanes:

Western blot - Anti-Neurofascin antibody [EPR19003] (ab184377) at 1/2000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Mouse spinal cord lysate at 10 µg

Lane 4:

Rat spinal cord lysate at 10 µg

Lane 5:

Mouse lung lysate at 10 µg

Lane 6:

Mouse heart lysate at 10 µg

Lane 7:

Mouse kidney lysate at 10 µg

Lane 8:

Rat kidney lysate at 10 µg

Lane 9:

Rat spleen lysate at 10 µg

Lane 10:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 11:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 12:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 150 kDa

Observed band size: 125 kDa,155 kDa,186 kDa,75 kDa

false

Exposure time: 5s

Carrier free

Anti-Neurofascin antibody [EPR19003] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19003

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

IHC is recommended for mouse and rat only.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofascin also known as NFASC is a cell adhesion molecule with significant roles in the nervous system. It has various isoforms with Neurofascin-186 and Neurofascin-155 being the most studied. The molecular mass of Neurofascin is approximately 135 kDa. This protein is expressed in the nervous system particularly at nodes of Ranvier and paranodal regions as well as in the initial segment of axons. Neurofascin contributes to organizing and maintaining the axonal domains essential for neural signal transmission.

Biological function summary

This neural protein plays an integral role in developing and securing the nervous system's structural organization. Neurofascin helps form the nodes of Ranvier which are critical for saltatory conduction enhancing rapid and efficient nerve impulse transmission. It also interacts with other proteins such as Ankyrin G and Contactin to maintain the stability and function of neuronal domains. Through these interactions Neurofascin facilitates the proper conduction and coordination of electrical signals along neurons.

Pathways

Neurofascin is essential for neural signal transmission and maintenance pathways. It prominently participates in the axon guidance and myelination pathways working alongside proteins like Caspr (Contactin-associated protein) and Ankyrin B. These pathways ensure the growth and maintenance of myelin sheaths supporting nerve insulation and efficient signal relay. Neurofascin's interaction with these proteins reinforces the nodes of Ranvier's organization promoting stable and rapid signal conduction in the peripheral and central nervous systems.

Research links Neurofascin with autoimmune neuropathies like Guillain-Barré syndrome and multiple sclerosis. These conditions involve the immune-mediated destruction of myelin leading to severe motor and sensory deficits. Neurofascin acts as a target in some autoimmune responses where antibodies may mistakenly attack the protein disrupting neural conduction. Connections between Neurofascin and other proteins like Contactin and Ankyrin G highlight the complex interactions that can lead to pathological states when disrupted. Developing therapies targeting Neurofascin-related pathways holds potential for mitigating these disorders' impacts on neuronal functions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.

See full target information NFASC

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:310 PubMed40128722

2025

Electroacupuncture attenuates intervertebral disc degeneration by upregulating aquaporins via the cAMP/PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Jia-Bao Huang,Jing Zou,Guo-Fu Huang

Scientific reports 13:16419 PubMed37775676

2023

Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats.

Applications

Unspecified application

Species

Unspecified reactive species

José Javier Miguel-Hidalgo,Erik Hearn,Mohadetheh Moulana,Khunsa Saleem,Austin Clark,Maggie Holmes,Kashish Wadhwa,Isabella Kelly,Craig Allen Stockmeier,Grazyna Rajkowska

Nature 617:369-376 PubMed37100909

2023

Inhibitory input directs astrocyte morphogenesis through glial GABAR.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Cheng,Estefania Luna-Figueroa,Junsung Woo,Hsiao-Chi Chen,Zhung-Fu Lee,Akdes Serin Harmanci,Benjamin Deneen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofascin antibody [EPR19003]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19003] (AB184377)

Western blot - Anti-Neurofascin antibody [EPR19003] (AB184377)

Western blot - Anti-Neurofascin antibody [EPR19003] (AB184377)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise