Anti-Neurofascin antibody [EPR19004]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neurofascin antibody [EPR19004] (AB184378)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Neurofascin with ab184378 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19004] (AB184378)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Neurofascin with ab184378 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative labeling on the epithelial cells of rat kidney is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19004] (AB184378)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Neurofascin with ab184378 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on the neuron cells of mouse hippocampus is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19004] (AB184378)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Neurofascin with ab184378 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on the neuron cells of rat hippocampus is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofascin antibody [EPR19004] (AB184378)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neurofascin with ab184378 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative labeling on the mouse liver is observed. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Neurofascin antibody [EPR19004] (AB184378)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight and expression profile observed are consistent with what has been described in the literature (PMID : 23160985).

All lanes:

Western blot - Anti-Neurofascin antibody [EPR19004] (ab184378) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat heart lysate at 10 µg

Lane 7:

Rat kidney lysate at 10 µg

Lane 8:

Rat spleen lysate at 10 µg

Lane 9:

C6 (Rat glial tumor cell line) lysate at 10 µg

Lane 10:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 11:

PC-12 (Rat adrenal gland pheochromocytoma cell line) lysate at 10 µg

Lane 12:

NIH/3T3 (Mouse embryonic fibroblast cell line) lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 150 kDa

Observed band size: 125 kDa,155 kDa,186 kDa

false

Exposure time: 3min