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AB250747

Anti-Neurofascin antibody [EPR19004] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NFASC antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Neurofascin, Nfasc

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-Neurofascin antibody [EPR19004] - BSA and Azide free (AB250747)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neurofascin antibody [EPR19004] - BSA and Azide free (AB250747)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Neurofascin with ab184378 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 μg/mL) (Green). Confocal image showing positive staining in mouse primary neuron cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 μg/mL) followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL) (Red). The nuclear counterstain was DAPI (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184378).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19004

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250747 is the carrier-free version of ab184378.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofascin also known as NFASC is a cell adhesion molecule with significant roles in the nervous system. It has various isoforms with Neurofascin-186 and Neurofascin-155 being the most studied. The molecular mass of Neurofascin is approximately 135 kDa. This protein is expressed in the nervous system particularly at nodes of Ranvier and paranodal regions as well as in the initial segment of axons. Neurofascin contributes to organizing and maintaining the axonal domains essential for neural signal transmission.
Biological function summary

This neural protein plays an integral role in developing and securing the nervous system's structural organization. Neurofascin helps form the nodes of Ranvier which are critical for saltatory conduction enhancing rapid and efficient nerve impulse transmission. It also interacts with other proteins such as Ankyrin G and Contactin to maintain the stability and function of neuronal domains. Through these interactions Neurofascin facilitates the proper conduction and coordination of electrical signals along neurons.

Pathways

Neurofascin is essential for neural signal transmission and maintenance pathways. It prominently participates in the axon guidance and myelination pathways working alongside proteins like Caspr (Contactin-associated protein) and Ankyrin B. These pathways ensure the growth and maintenance of myelin sheaths supporting nerve insulation and efficient signal relay. Neurofascin's interaction with these proteins reinforces the nodes of Ranvier's organization promoting stable and rapid signal conduction in the peripheral and central nervous systems.

Research links Neurofascin with autoimmune neuropathies like Guillain-Barré syndrome and multiple sclerosis. These conditions involve the immune-mediated destruction of myelin leading to severe motor and sensory deficits. Neurofascin acts as a target in some autoimmune responses where antibodies may mistakenly attack the protein disrupting neural conduction. Connections between Neurofascin and other proteins like Contactin and Ankyrin G highlight the complex interactions that can lead to pathological states when disrupted. Developing therapies targeting Neurofascin-related pathways holds potential for mitigating these disorders' impacts on neuronal functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.
See full target information Nfasc
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 24:1541-1557 PubMed36192632

2022

Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana D Larionova,Soniya Bastola,Tatiana E Aksinina,Ksenia S Anufrieva,Jia Wang,Victoria O Shender,Dmitriy E Andreev,Tatiana F Kovalenko,Georgij P Arapidi,Polina V Shnaider,Anastasia N Kazakova,Yaroslav A Latyshev,Victor V Tatarskiy,Alexander A Shtil,Pascale Moreau,Francis Giraud,Chaoxi Li,Yichan Wang,Maria P Rubtsova,Olga A Dontsova,Michael Condro,Benjamin M Ellingson,Mikhail I Shakhparonov,Harley I Kornblum,Ichiro Nakano,Marat S Pavlyukov
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com