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AB128054

Anti-Neurofibromin antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Neurofibromin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NF1.

View Alternative Names

Neurofibromin, Neurofibromatosis-related protein NF-1, NF1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (AB128054)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (AB128054)

ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human spleen tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (AB128054)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofibromin antibody (AB128054)

ab128054, at a dilution of 1/100, staining Neurofibromin in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-Neurofibromin antibody (AB128054)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Neurofibromin antibody (AB128054)

ab128054, at a dilution of 1/100, staining Neurofibromin in HepG2 cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.

Western blot - Anti-Neurofibromin antibody (AB128054)
  • WB

Unknown

Western blot - Anti-Neurofibromin antibody (AB128054)

All lanes:

Western blot - Anti-Neurofibromin antibody (ab128054) at 1/500 dilution

Lane 1:

HepG2 cells extract

Lane 2:

HepG2 cells extract with synthesized peptide

Predicted band size: 319 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NF1. The exact immunogen used to generate this antibody is proprietary information.

P21359

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofibromin is a protein that functions as a Ras GTPase-activating protein acting to promote the hydrolysis of Ras-bound GTP to GDP which inactivates Ras signaling. This regulatory function involves the suppression of excessive cell proliferation and differentiation. Alternate names for neurofibromin include NF1 or neurofibromin 1. The mass of neurofibromin is approximately 327 kDa. Neurofibromin is expressed widely across various tissues with notable expression in the nervous system and the brain.
Biological function summary

Neurofibromin regulates pathways involved in cell growth and development. It does not form part of a large multiprotein complex but interacts with microtubules and other signaling proteins to carry out its regulatory functions. Neurofibromin primarily affects pathways that manage cell cycle progression and it plays a critical role in inhibiting uncontrolled cell growth ensuring proper cellular homeostasis.

Pathways

Neurofibromin fits into the Ras-MAPK pathway where it controls the activity of Ras an important regulatory protein involved in signal transduction related to cell division and differentiation. Neurofibromin's action on Ras directly impacts the activation of the MAPK pathway which is important in mediating cellular responses to growth signals. This protein also interacts functionally with proteins like SOS1 another regulator of Ras emphasizing its role in fine-tuning signal cascades that ensure balanced cellular function.

Neurofibromin's dysregulation is most commonly associated with neurofibromatosis type 1 a genetic disorder characterized by the development of benign tumors in the nervous system. The loss of neurofibromin function leads to unregulated Ras activity resulting in tumorigenesis. Additionally neurofibromin is implicated in certain sporadic cancers due to its role in controlling cell proliferation. In neurofibromatosis type 1 interactions between neurofibromin and proteins such as p53 a known tumor suppressor highlight its significance in disease pathways and tumor suppression mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
See full target information NF1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:1732-1747 PubMed35510668

2022

Intracerebral hemorrhage and thrombin-induced alterations in cerebral microvessel matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Huan Gu,Brian T Hawkins,Yoshikane Izawa,Yoji Yoshikawa,James A Koziol,Gregory J Del Zoppo

Experimental and therapeutic medicine 22:1262 PubMed34603530

2021

Autophagy is involved in neurofibromatosis type I gene-modulated osteogenic differentiation in human bone mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqiang Li,Mingwei Zhu,Xuemei Lin,Jingchun Li,Zhe Yuan,Yanhan Liu,Hongwen Xu

Cell reports 31:107502 PubMed32294438

2020

Molecular Analysis of Clinically Defined Subsets of High-Grade Serous Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghoon Lee,Li Zhao,Christine Rojas,Nicholas W Bateman,Hui Yao,Olivia D Lara,Joseph Celestino,Margaret B Morgan,Tri V Nguyen,Kelly A Conrads,Kelly M Rangel,Robert L Dood,Richard A Hajek,Gloria L Fawcett,Randy A Chu,Katlin Wilson,Jeremy L Loffredo,Coralie Viollet,Amir A Jazaeri,Clifton L Dalgard,Xizeng Mao,Xingzhi Song,Ming Zhou,Brian L Hood,Nirad Banskota,Matthew D Wilkerson,Jerez Te,Anthony R Soltis,Kristin Roman,Andrew Dunn,David Cordover,Agda Karina Eterovic,Jinsong Liu,Jared K Burks,Keith A Baggerly,Nicole D Fleming,Karen H Lu,Shannon N Westin,Robert L Coleman,Gordon B Mills,Yovanni Casablanca,Jianhua Zhang,Thomas P Conrads,George L Maxwell,P Andrew Futreal,Anil K Sood
View all publications
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