Knockout Tested Rabbit Recombinant Monoclonal Neurofibromin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img6363.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004)")
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img11644.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004)")
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img419782.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (AB260004)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
1 products for Alternative Version
Target data
Function
Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.
Alternative names
Neurofibromin, Neurofibromatosis-related protein NF-1, NF1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal Neurofibromin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Alternative names
Neurofibromin, Neurofibromatosis-related protein NF-1, NF1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR22989-68
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab260004 is the carrier-free version of Anti-TrkB (phospho Y705) antibody [EPR22298-67] - BSA and Azide free ab264070.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Neurofibromin is a protein that functions as a Ras GTPase-activating protein acting to promote the hydrolysis of Ras-bound GTP to GDP which inactivates Ras signaling. This regulatory function involves the suppression of excessive cell proliferation and differentiation. Alternate names for neurofibromin include NF1 or neurofibromin 1. The mass of neurofibromin is approximately 327 kDa. Neurofibromin is expressed widely across various tissues with notable expression in the nervous system and the brain.
- Biological function summary
Neurofibromin regulates pathways involved in cell growth and development. It does not form part of a large multiprotein complex but interacts with microtubules and other signaling proteins to carry out its regulatory functions. Neurofibromin primarily affects pathways that manage cell cycle progression and it plays a critical role in inhibiting uncontrolled cell growth ensuring proper cellular homeostasis.
- Pathways
Neurofibromin fits into the Ras-MAPK pathway where it controls the activity of Ras an important regulatory protein involved in signal transduction related to cell division and differentiation. Neurofibromin's action on Ras directly impacts the activation of the MAPK pathway which is important in mediating cellular responses to growth signals. This protein also interacts functionally with proteins like SOS1 another regulator of Ras emphasizing its role in fine-tuning signal cascades that ensure balanced cellular function.
- Associated diseases and disorders
Neurofibromin's dysregulation is most commonly associated with neurofibromatosis type 1 a genetic disorder characterized by the development of benign tumors in the nervous system. The loss of neurofibromin function leads to unregulated Ras activity resulting in tumorigenesis. Additionally neurofibromin is implicated in certain sporadic cancers due to its role in controlling cell proliferation. In neurofibromatosis type 1 interactions between neurofibromin and proteins such as p53 a known tumor suppressor highlight its significance in disease pathways and tumor suppression mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img6363.jpg "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)")
Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Neurofibromin antibody [EPR22989-68] ab238142).
Lanes 1-3: Merged signal (red and green). Green - Anti-Neurofibromin antibody [EPR22989-68] ab238142 observed at 319 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-Neurofibromin antibody [EPR22989-68] ab238142 Recombinant Anti-NF1 antibody [EPR22989-68] was shown to specifically react with NFIB in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NF1 (Neurofibromin) knockout HeLa cell line ab264725 (knockout cell lysate Human NF1 (Neurofibromin) knockout HeLa cell lysate ab258533) was used. Wild-type and NF1 knockout samples were subjected to SDS-PAGE. Anti-Neurofibromin antibody [EPR22989-68] ab238142 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Neurofibromin antibody [EPR22989-68] (Anti-Neurofibromin antibody [EPR22989-68] ab238142) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: NFIB knockout HeLa cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 319 kDa
Observed band size: 319 kDa
Lanes 1-3: Merged signal (red and green). Green - Anti-Neurofibromin antibody [EPR22989-68] ab238142 observed at 319 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-Neurofibromin antibody [EPR22989-68] ab238142 Recombinant Anti-NF1 antibody [EPR22989-68] was shown to specifically react with NFIB in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human NF1 (Neurofibromin) knockout HeLa cell line ab264725 (knockout cell lysate Human NF1 (Neurofibromin) knockout HeLa cell lysate ab258533) was used. Wild-type and NF1 knockout samples were subjected to SDS-PAGE. Anti-Neurofibromin antibody [EPR22989-68] ab238142 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img11644.jpg "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)")
Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)
Anti-Neurofibromin antibody [EPR22989-68] ab238142 was shown to specifically react with Neurofibromin in wild-type HAP1 cells as signal was lost in Neurofibromin knockout cells. Wild-type and Neurofibromin knockout samples were subjected to SDS-PAGE. Anti-Neurofibromin antibody [EPR22989-68] ab238142 and Anti-Vinculin antibody [EPR8185] ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique. Lysate should be made freshly and used in WB immediately to minimize protein degradation. Degraded fragment(250 KD) observed is consistent with what has been described in the literature (PMID: 30131853, PMID: 30408279).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 26 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurofibromin antibody [EPR22989-68] ab238142).
All lanes: Western blot - Anti-Neurofibromin antibody [EPR22989-68] (Anti-Neurofibromin antibody [EPR22989-68] ab238142) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Neurofibromin knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 319 kDa
Observed band size: 250 kDa, 300 kDa
![Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004), expandable thumbnail](https://content.abcam.com/products/images/neurofibromin-antibody-epr22989-68-bsa-and-azide-free-ab260004--western-blot-img419782.jpg "Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)")
Western blot - Anti-Neurofibromin antibody [EPR22989-68] - BSA and Azide free (ab260004)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Neurofibromin antibody [EPR22989-68] ab238142).
Anti-NF1 antibody [EPR22989-68] (Anti-Neurofibromin antibody [EPR22989-68] ab238142) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Neurofibromin antibody [EPR22989-68] ab238142 was shown to bind specifically to NF1. A band was observed at 180-270 kDa in wild-type MCF7 cell lysates with no signal observed at this size in NF1 knockout cell line. To generate this image, wild-type and NF1 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.All lanes: Western blot - Anti-Neurofibromin antibody [EPR22989-68] (Anti-Neurofibromin antibody [EPR22989-68] ab238142) at 1/1000 dilution
All lanes: Western blot at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 319 kDa
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