AB204893

Anti-Neurofilament antibody

Be the first to review this product! Submit a review

|

(11 Publications)

Rabbit Polyclonal NFM antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NEFH aa 400-500.

View Alternative Names

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

Immunohistochemical analysis of human cerebral cortex tissue labeling neurofilament in the membrane of neuropil with ab204893 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

Immunohistochemical analysis of human liver tissue labeling neurofilament with ab204893 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

Immunohistochemical analysis of human kidney tissue labeling neurofilament with ab204893 at a 1/50 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

Immunohistochemical analysis of human testis tissue labeling neurofilament in the cytoplasm of seminiferous ducts with ab204893 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody (AB204893)

Immunohistochemical analysis of human cerebellum tissue labeling neurofilament in the cytoplasm of Purkinje cells with ab204893 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

Immunogen

Recombinant Fragment Protein within Human NEFH aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament is a type of intermediate filament found in neurons predominantly within the axons. Known also as neurofilament protein it has several subunits including NF-L (neurofilament light) NF-M (medium) and NF-H (heavy) with NF-L often used in research contexts. NF-L mouse models and antibodies such as anti-NFL help in the study of neuronal structure. The mass of NF-L is about 68 kilodaltons. Neurofilament protein is highly expressed in neurons and plays an important role in maintaining the structural framework of the axon.

Biological function summary

Neurofilaments are important for providing structural support to neurons and are involved in maintaining the caliber of axons. They form a complex with the other intermediate filaments and microtubules which are essential for proper neuronal function. This complex is vital for the assembly and structural integrity of the cytoskeleton affecting intracellular transport and cellular mechanics ensuring proper communication within neural circuits.

Pathways

Neurofilaments function in the neuronal cytoskeleton maintenance and are involved in the motor protein pathways. They interact with proteins such as kinesin and dynein for axonal transport which is essential for moving organelles proteins and other cargoes between the cell body and axon terminals. Neurofilament proteins acting through these pathways ensure effective communication across neuronal networks impacting overall nerve function and health.

Neurofilament levels are valuable biomarkers for neurological conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. In Alzheimer's disease neurofilament pathology outlines alterations that associate with tau and amyloid-beta proteins which are hallmark features of the disorder. Similarly in ALS elevated neurofilament levels indicate neuronal damage correlating with the disease's progression and severity. Neurofilament staining in tissues via immunohistochemistry (neurofilament IHC) provides insights into these diseases aiding both diagnosis and monitoring of disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFM

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Hypertension research : official journal of the Japanese Society of Hypertension 48:2083-2094 PubMed40335722

2025

Identification of putative baroreceptors in human aortic arch by histological and omics analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Yankey Yundung,Jaroslav Pelisek,Umberto Maccio,Zsuzsanna Varga,Houria Ech-Cherif,Enni Markkanen,Fabian Rössler,Ahmed Ouda,Omer Dzemali,Alexander Zimmermann,Benedikt Reutersberg

Molecular medicine (Cambridge, Mass.) 30:106 PubMed39039432

2024

Modulating the RPS27A/PSMD12/NF-κB pathway to control immune response in mouse brain ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocheng Li,Ming Qiao,Yan Zhou,Yan Peng,Gang Wen,Chenchen Xie,Yamei Zhang

Neural development 18:7 PubMed37833718

2023

Neocortex neurogenesis and maturation in the African greater cane rat.

Applications

Unspecified application

Species

Unspecified reactive species

Oluwaseun Mustapha,Thomas Grochow,James Olopade,Simone A Fietz

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2205744 PubMed36808712

2023

3D Printed Conductive Multiscale Nerve Guidance Conduit with Hierarchical Fibers for Peripheral Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yongcong Fang,Chengjin Wang,Zibo Liu,Jeonghoon Ko,Li Chen,Ting Zhang,Zhuo Xiong,Lei Zhang,Wei Sun

Cancer cell 40:1521-1536.e7 PubMed36400020

2022

Molecular classification and biomarkers of clinical outcome in breast ductal carcinoma in situ: Analysis of TBCRC 038 and RAHBT cohorts.

Applications

Unspecified application

Species

Unspecified reactive species

Siri H Strand,Belén Rivero-Gutiérrez,Kathleen E Houlahan,Jose A Seoane,Lorraine M King,Tyler Risom,Lunden A Simpson,Sujay Vennam,Aziz Khan,Luis Cisneros,Timothy Hardman,Bryan Harmon,Fergus Couch,Kristalyn Gallagher,Mark Kilgore,Shi Wei,Angela DeMichele,Tari King,Priscilla F McAuliffe,Julie Nangia,Joanna Lee,Jennifer Tseng,Anna Maria Storniolo,Alastair M Thompson,Gaorav P Gupta,Robyn Burns,Deborah J Veis,Katherine DeSchryver,Chunfang Zhu,Magdalena Matusiak,Jason Wang,Shirley X Zhu,Jen Tappenden,Daisy Yi Ding,Dadong Zhang,Jingqin Luo,Shu Jiang,Sushama Varma,Lauren Anderson,Cody Straub,Sucheta Srivastava,Christina Curtis,Rob Tibshirani,Robert Michael Angelo,Allison Hall,Kouros Owzar,Kornelia Polyak,Carlo Maley,Jeffrey R Marks,Graham A Colditz,E Shelley Hwang,Robert B West

Scientific reports 10:12789 PubMed32732905

2020

Intracellular G-actin targeting of peripheral sensory neurons by the multifunctional engineered protein C2C confers relief from inflammatory pain.

Applications

Unspecified application

Species

Unspecified reactive species

Derek Allen,You Zhou,Audrey Wilhelm,Paul Blum

Journal of nanobiotechnology 18:105 PubMed32711535

2020

Neuron-derived exosomes-transmitted miR-124-3p protect traumatically injured spinal cord by suppressing the activation of neurotoxic microglia and astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Jiang,Fangyi Gong,Xuhui Ge,Chengtang Lv,Chenyu Huang,Shuang Feng,Zheng Zhou,Yuluo Rong,Jiaxing Wang,Chengyue Ji,Jian Chen,Wene Zhao,Jin Fan,Wei Liu,Weihua Cai

Annals of transplantation 25:e924093 PubMed32686658

2020

Self-Assembling Peptide Scaffold Carrying Neural-Cell Adhesion Molecule-Derived Mimetic-Peptide Transplantation Promotes Proliferation and Stimulates Neurite Extension by Modulating Tau Phosphorylation and Calpain/Glycogen Synthase Kinase 3 beta (GSK-3β) in Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Jing Feng,Yu-Dong Liu,Tao Hu,Ming-Jing Li,Fan Li

Journal of neurochemistry 156:367-378 PubMed32621322

2020

Epigenetic restoration of voltage-gated potassium channel Kv1.2 alleviates nerve injury-induced neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Lina Rong,Jinping Shao,Yidan Zhang,Yaping Liu,Sen Zhao,Lei Li,Wenli Yu,Mengya Zhang,Xiuhua Ren,Qingzan Zhao,Changlian Zhu,Huan Luo,Weidong Zang,Jing Cao

Bosnian journal of basic medical sciences 21:98-110 PubMed32156249

2020

Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nur Izzati Mansor,Carolindah Makena Ntimi,Noraishah Mydin Abdul-Aziz,King-Hwa Ling,Aishah Adam,Rozita Rosli,Zurina Hassan,Norshariza Nordin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com