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AB252585

Anti-Neurofilament antibody [EPR20020-137] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal NFM antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

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Sandwich ELISA - Anti-Neurofilament antibody [EPR20020-137] - BSA and Azide free (Capture) (AB252585)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Neurofilament antibody [EPR20020-137] - BSA and Azide free (Capture) (AB252585)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272199).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Neurofilament heavy polypeptide antibody [EPR20020]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20020-137

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab252585 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurofilament H. The recommended pair for sandwich ELISA is:
Capture: ab252585, Human Neurofilament H Capture Antibody (unconjugated)
Detector: ab272366, Human Neurofilament H Detector Antibody (unconjugated)
The reference range value is 0.9375 - 60 ng/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament is a type of intermediate filament found in neurons predominantly within the axons. Known also as neurofilament protein it has several subunits including NF-L (neurofilament light) NF-M (medium) and NF-H (heavy) with NF-L often used in research contexts. NF-L mouse models and antibodies such as anti-NFL help in the study of neuronal structure. The mass of NF-L is about 68 kilodaltons. Neurofilament protein is highly expressed in neurons and plays an important role in maintaining the structural framework of the axon.
Biological function summary

Neurofilaments are important for providing structural support to neurons and are involved in maintaining the caliber of axons. They form a complex with the other intermediate filaments and microtubules which are essential for proper neuronal function. This complex is vital for the assembly and structural integrity of the cytoskeleton affecting intracellular transport and cellular mechanics ensuring proper communication within neural circuits.

Pathways

Neurofilaments function in the neuronal cytoskeleton maintenance and are involved in the motor protein pathways. They interact with proteins such as kinesin and dynein for axonal transport which is essential for moving organelles proteins and other cargoes between the cell body and axon terminals. Neurofilament proteins acting through these pathways ensure effective communication across neuronal networks impacting overall nerve function and health.

Neurofilament levels are valuable biomarkers for neurological conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. In Alzheimer's disease neurofilament pathology outlines alterations that associate with tau and amyloid-beta proteins which are hallmark features of the disorder. Similarly in ALS elevated neurofilament levels indicate neuronal damage correlating with the disease's progression and severity. Neurofilament staining in tissues via immunohistochemistry (neurofilament IHC) provides insights into these diseases aiding both diagnosis and monitoring of disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFM
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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