AB215903

Anti-Neurofilament antibody [NF421 + NFL/736]

Name: Anti-Neurofilament antibody [NF421 + NFL/736] (ab215903) | Abcam
Brand: Abcam Limited
SKU: AB215903
Price: 535 USD
Availability: InStock

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(9 Publications)

Mouse Multiclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-P, ICC, Flow Cyt and reacts with Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEFH.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Flow Cyt

Unknown

Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Flow Cytometric Analysis of HEK293 cells using ab215903 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Supplier Data

Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Immunofluorescence staining of HEK293 cells using ab215903 followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat cerebellum tissue labeling Neurofilament with ab215903 at 1 μg/ml dilution.

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

NF421 + NFL/736

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEFH.

P12036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^5 Cells", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab215903 is purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament is a type of intermediate filament found in neurons predominantly within the axons. Known also as neurofilament protein it has several subunits including NF-L (neurofilament light) NF-M (medium) and NF-H (heavy) with NF-L often used in research contexts. NF-L mouse models and antibodies such as anti-NFL help in the study of neuronal structure. The mass of NF-L is about 68 kilodaltons. Neurofilament protein is highly expressed in neurons and plays an important role in maintaining the structural framework of the axon.

Biological function summary

Neurofilaments are important for providing structural support to neurons and are involved in maintaining the caliber of axons. They form a complex with the other intermediate filaments and microtubules which are essential for proper neuronal function. This complex is vital for the assembly and structural integrity of the cytoskeleton affecting intracellular transport and cellular mechanics ensuring proper communication within neural circuits.

Pathways

Neurofilaments function in the neuronal cytoskeleton maintenance and are involved in the motor protein pathways. They interact with proteins such as kinesin and dynein for axonal transport which is essential for moving organelles proteins and other cargoes between the cell body and axon terminals. Neurofilament proteins acting through these pathways ensure effective communication across neuronal networks impacting overall nerve function and health.

Neurofilament levels are valuable biomarkers for neurological conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. In Alzheimer's disease neurofilament pathology outlines alterations that associate with tau and amyloid-beta proteins which are hallmark features of the disorder. Similarly in ALS elevated neurofilament levels indicate neuronal damage correlating with the disease's progression and severity. Neurofilament staining in tissues via immunohistochemistry (neurofilament IHC) provides insights into these diseases aiding both diagnosis and monitoring of disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28482 PubMed40759713

2025

The inhibitory effect of M2 macrophage-derived exosomes on gefitinib resistant lung adenocarcinoma cells through the MIF/TIMP1/CD74 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingcui Peng,Yan Zhang,Bin Li,Xin He,Cuimin Ding,Wenxia Hu

Osteoarthritis and cartilage open 7:100649 PubMed40735537

2025

Comorbid hypertension and osteoarthritis exacerbates joint remodeling and gait compensations in female rats with milder effects observed in males.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos J Cruz,Folly M Patterson,Janak Gaire,Julianna Gonzalez,Jacob L Griffith,Angela Philistin,Kyle D Allen

Cells 14: PubMed40214483

2025

Induced Pluripotent Stem Cell-Derived Exosomes Promote Peripheral Nerve Regeneration in a Rat Sciatic Nerve Crush Injury Model: A Safety and Efficacy Study.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Aldali,Yujie Yang,Chunchu Deng,Xiangling Li,Xiaojian Cao,Jia Xu,Yajie Li,Jianlin Ding,Hong Chen

Heliyon 10:e37231 PubMed39296039

2024

PMMA-induced biofilm promotes Schwann cells migration and proliferation mediated by EGF/Tnc/FN1 to improve sciatic nerve defect.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,YuXuan Hu,Yuan Xue,Kai Wang,Dong Mao,Xiao-Yun Pan,YongJun Rui

Cell communication and signaling : CCS 22:307 PubMed38831315

2024

Interleukin-22 receptor 1-mediated stimulation of T-type Ca channels enhances sensory neuronal excitability through the tyrosine-protein kinase Lyn-dependent PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Cai,Siyu Chen,Yufang Sun,Tingting Zheng,Yulu Liu,Jin Tao,Yuan Zhang

The journal of headache and pain 24:49 PubMed37158881

2023

Trace amine-associated receptor 1 regulation of Kv1.4 channels in trigeminal ganglion neurons contributes to nociceptive behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Hua Wang,Yufang Sun,Zitong Huang,Yu Tao,Yiru Wang,Xinghong Jiang,Jin Tao

Scientific reports 12:10026 PubMed35705577

2022

Functional, morphological and molecular characteristics in a novel rat model of spinal sacral nerve injury-surgical approach, pathological process and clinical relevance.

Applications

Unspecified application

Species

Unspecified reactive species

Junyang Li,Shiqiang Li,Yu Wang,Aijia Shang

Theranostics 12:2232-2247 PubMed35265208

2022

Interleukin 33-mediated inhibition of A-type K channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yiru Wang,Xinyi Wang,Renfei Qi,Ying Lu,Yu Tao,Dongsheng Jiang,Yufang Sun,Xinghong Jiang,Chunfeng Liu,Yuan Zhang,Jin Tao

Stem cell research & therapy 12:442 PubMed34362437

2021

Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Yang,Yang Yang,Yichi Xu,Weiqian Jiang,Yan Shao,Jiahua Xing,Youbai Chen,Yan Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament antibody [NF421 + NFL/736]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Flow Cytometry - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Immunocytochemistry - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [NF421 + NFL/736] (AB215903)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise