AB134306

Anti-Neurofilament antibody [RNF406] - Neuronal Marker

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(11 Publications)

Mouse Monoclonal NFM antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Tissue preparation containing NEFM protein.

View Alternative Names

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (AB134306)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (AB134306)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue labelling 140 kD + 160 kD Neurofilament with ab134306 at 10 μg/ml, using a biotinylated horse anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Lab

Western blot - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (AB134306)

Lanes 1 - 3 : Merged signal (red and green). Green - ab134306 observed at 140-160 kDa kDa. Red - loading control, ab52866, observed at 50 kDa.

ab134306 was shown to specifically react with Neurofilament in wild-type HEK293 cells as signal was lost in Neurofilament knockout cells. Wild-type and Neurofilament knockout samples were subjected to SDS-PAGE. ab134306 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (ab134306) at 1/100 dilution

Lane 1:

Wild-type HEK293 whole cell lysate at 20 µg

Lane 2:

Neurofilament knockout HEK293 whole cell lysate at 20 µg

Lane 3:

A549 whole cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 140-160 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RNF406

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Tissue preparation containing NEFM protein. The exact immunogen used to generate this antibody is proprietary information.

O77788

Specificity

ab134306 reacts exclusively with the phosphorylated isoform of the 160 kDa neurofilament protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament is a type of intermediate filament found in neurons predominantly within the axons. Known also as neurofilament protein it has several subunits including NF-L (neurofilament light) NF-M (medium) and NF-H (heavy) with NF-L often used in research contexts. NF-L mouse models and antibodies such as anti-NFL help in the study of neuronal structure. The mass of NF-L is about 68 kilodaltons. Neurofilament protein is highly expressed in neurons and plays an important role in maintaining the structural framework of the axon.

Biological function summary

Neurofilaments are important for providing structural support to neurons and are involved in maintaining the caliber of axons. They form a complex with the other intermediate filaments and microtubules which are essential for proper neuronal function. This complex is vital for the assembly and structural integrity of the cytoskeleton affecting intracellular transport and cellular mechanics ensuring proper communication within neural circuits.

Pathways

Neurofilaments function in the neuronal cytoskeleton maintenance and are involved in the motor protein pathways. They interact with proteins such as kinesin and dynein for axonal transport which is essential for moving organelles proteins and other cargoes between the cell body and axon terminals. Neurofilament proteins acting through these pathways ensure effective communication across neuronal networks impacting overall nerve function and health.

Neurofilament levels are valuable biomarkers for neurological conditions such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. In Alzheimer's disease neurofilament pathology outlines alterations that associate with tau and amyloid-beta proteins which are hallmark features of the disorder. Similarly in ALS elevated neurofilament levels indicate neuronal damage correlating with the disease's progression and severity. Neurofilament staining in tissues via immunohistochemistry (neurofilament IHC) provides insights into these diseases aiding both diagnosis and monitoring of disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFM

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:224 PubMed38724500

2024

Salidroside promotes the repair of spinal cord injury by inhibiting astrocyte polarization, promoting neural stem cell proliferation and neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Dingfei Qian,Yuan Dong,Xiaole Liu,Haichao Yu,Zelong Song,Chengqi Jia,Zhen Zhang,Shiqi Cao,Fanqi Hu,Xuesong Zhang

Biochemistry and biophysics reports 35:101497 PubMed37534324

2023

Umbilical mesenchymal stem cell-derived exosomes promote spinal cord functional recovery through the miR-146b/TLR4 -mediated NF-κB p65 signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiujuan Wang,Ying Yang,Wei Li,MingYuan Hao,YongSheng Xu

Materials today. Bio 20:100616 PubMed37025556

2023

Photocatalytic oxygen evolution and antibacterial biomimetic repair membrane for diabetes wound repair via HIF1-α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Su,Bing Ye,Ziming Zhang,Qing Gao,Lian Zeng,Yizhou Wan,Wenzhe Sun,Siyue Chen,Daping Quan,Jialin Yu,Xiaodong Guo

Materials today. Bio 16:100434 PubMed36186848

2022

Aptamer engineering exosomes loaded on biomimetic periosteum to promote angiogenesis and bone regeneration by targeting injured nerves via JNK3 MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Su,Qing Gao,Rongli Deng,Lian Zeng,Jingyi Guo,Bing Ye,Jialin Yu,Xiaodong Guo

Bioengineering & translational medicine 7:e10287 PubMed35600663

2022

Exosomes derived from olfactory ensheathing cells provided neuroprotection for spinal cord injury by switching the phenotype of macrophages/microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Fan,Zhe Chen,Hai-Bin Tang,Le-Qun Shan,Zi-Yi Chen,Xiao-Hui Wang,Da-Geng Huang,Shi-Chang Liu,Xun Chen,Hao Yang,Dingjun Hao

Experimental and therapeutic medicine 23:291 PubMed35317450

2022

S100A9 blockade improves the functional recovery after spinal cord injury via mediating neutrophil infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Sun,Haiwei Zhang,Tianwen Huang,Jianhui Shi,Tianli Wei,Yansong Wang

Molecular therapy. Nucleic acids 22:1063-1077 PubMed33294293

2020

MicroRNA-135a-5p Promotes the Functional Recovery of Spinal Cord Injury by Targeting SP1 and ROCK.

Applications

Unspecified application

Species

Unspecified reactive species

Nanxiang Wang,Yang Yang,Mao Pang,Cong Du,Yuyong Chen,Simin Li,Zhenming Tian,Feng Feng,Yang Wang,Zhenxiang Chen,Bin Liu,Limin Rong

Drug design, development and therapy 14:2695-2705 PubMed32753848

2020

Ulinastatin Promotes Regeneration of Peripheral Nerves After Sciatic Nerve Injury by Targeting let-7 microRNAs and Enhancing NGF Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jincheng Zhang,Yingqi Zhang,Lei Chen,Zhitao Rao,Yeqing Sun

Neuroscience bulletin 35:791-801 PubMed30980241

2019

Decreased miR-325-5p Contributes to Visceral Hypersensitivity Through Post-transcriptional Upregulation of CCL2 in Rat Dorsal Root Ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Wu,Ping-An Zhang,Xuelian Liu,Yuan Zhou,Meijie Xu,Xinghong Jiang,Jun Yan,Guang-Yin Xu

Gut 66:1767-1778 PubMed28096305

2017

Epithelial expression and function of trypsin-3 in irritable bowel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Rolland-Fourcade,Alexandre Denadai-Souza,Carla Cirillo,Cintya Lopez,Josue Obed Jaramillo,Cleo Desormeaux,Nicolas Cenac,Jean-Paul Motta,Muriel Larauche,Yvette Taché,Pieter Vanden Berghe,Michel Neunlist,Emmanuel Coron,Sylvain Kirzin,Guillaume Portier,Delphine Bonnet,Laurent Alric,Stephen Vanner,Celine Deraison,Nathalie Vergnolle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament antibody [RNF406] - Neuronal Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (AB134306)

Western blot - Anti-Neurofilament antibody [RNF406] - Neuronal Marker (AB134306)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise