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Mouse Monoclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NEFH aa 1-250.

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Images

Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (AB19386), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human NEFH aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P12036

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Expected
Rat
Tested
Tested
Tested

Tested
Tested

Species

Rat

Dilution info
1/10.00000 - 1/100.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species

Human

Dilution info
1/10.00000 - 1/100.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Rat

Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Species

Human

Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Tested
Tested

Species

Rat

Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

Recommended products

Mouse Monoclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NEFH aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human NEFH aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P12036
Clone number

3G3

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Associated diseases and disorders

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386), expandable thumbnail

    Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

    Results show a band at ~200 kDa.

    All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386) at 1/500 dilution

    Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 25 µg

    Lane 2: Rat brain tissue lysates at 25 µg

    Secondary

    All lanes: HRP-conjugated secondary antibody

    Predicted band size: 112 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

    Immunohistochemistry analysis of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) labelling Neurofilament Heavy Chain with ab19386. Positive staining in the cytoplasm and nucleus (right).
    To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Neurofilament Heavy Chain monoclonal antibody diluted in 3% BSA-PBS at 1/20 dilution overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

    Immunohistochemistry analysis of Neurofilament, Heavy chain showing staining in the cytoplasm and axons of formalin-fixed, paraffin-embedded human brain tissue (B) and magnified section (C) compared with an isotype control (A). To expose target proteins, antigen retrieval was performed using HEIR with a buffer (pH 6.2). Tissues were probed with a Neurofilament, Heavy chain monoclonal antibody for 60 minutes at a dilution of 2 μg/mL and detection was performed using an HRP-conjugated detection system for 30 minutes followed by DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [3G3] (ab19386)

    Immunofluorescence of neurofilament, heavy chain in rat cerebral cortex cultures in green.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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