AB134459

Anti-Neurofilament heavy polypeptide antibody

Name: Anti-Neurofilament heavy polypeptide antibody (ab134459) | Abcam
Brand: Abcam Limited
SKU: AB134459
Price: 525 USD
Availability: InStock

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(4 Publications)

Chicken Polyclonal Neurofilament heavy polypeptide antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Nfh, Nefh, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (AB134459)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (AB134459)

Immunofluorescent analysis of dissociated cell culture prepared from a neo-natal mouse brain labelling Neurofilament heavy polypeptide with ab134459 at 1/5000 dilution followed by Secondary antibody (fluorescein-labeled goat anti-chicken IgY used at 1/1000 dilution. Neurofilament heavy polypeptide (green staining) can be found within the neuronal cell body and in the neurites.

Picture courtesy of Dr. Gerry Shaw (University of Florida).

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000 - 1/5000", "ICCIF-species-notes": "<p>Use 2% paraformaldehyde-fixed cells.</p>" } } }

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Product details

Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Purification notes

Immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in buffer, and then filter-sterilized.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NEF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information Nefh

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Aging biology 1: PubMed38500536

2024

Early-Onset Hearing Loss in Mouse Models of Alzheimer's Disease and Increased DNA Damage in the Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Hyeon Park,Burcin Duan Sahbaz,Komal Pekhale,Xixia Chu,Mustafa N Okur,Mhamed Grati,Kevin Isgrig,Wade Chien,Elena Chrysostomou,Lauren Sullivan,Deborah L Croteau,Uri Manor,Vilhelm A Bohr

Frontiers in neuroscience 18:1361590 PubMed38406586

2024

Enhancing regeneration and repair of long-distance peripheral nerve defect injuries with continuous microcurrent electrical nerve stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Kong,Cheng Teng,Fenglan Liu,Xuzhaoyu Wang,Yi Zhou,Ying Zong,Zixin Wan,Jun Qin,Bin Yu,Daguo Mi,Yaxian Wang

PloS one 14:e0224022 PubMed31671109

2019

BDNF, NT-3 and Trk receptor agonist monoclonal antibodies promote neuron survival, neurite extension, and synapse restoration in rat cochlea ex vivo models relevant for hidden hearing loss.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Szobota,Pranav D Mathur,Sairey Siegel,KristenAnn Black,H Uri Saragovi,Alan C Foster

Scientific reports 7:17428 PubMed29233995

2017

Adeno-associated virus serotype rh10 is a useful gene transfer vector for sensory nerves that innervate bone in immunodeficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sun H Park,Matthew R Eber,Shunsuke Tsuzuki,Mary E Booker,Aaron G Sunil,D Brooke Widner,Renee A Parker,Christopher M Peters,Yusuke Shiozawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Neurofilament heavy polypeptide antibody

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (AB134459)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise