Anti-Neurofilament heavy polypeptide antibody ab4680 is a chicken polyclonal antibody that is used in Neurofilament heavy polypeptide western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
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- Journal of neuroinflammation 20:2582023M2 macrophage-derived cathepsin S promotes peripheral nerve regeneration via fibroblast-Schwann cell-signaling relay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEri Oshima,Yoshinori Hayashi,Zhen Xie,Hitoshi Sato,Suzuro Hitomi,Ikuko Shibuta,Kentaro Urata,Junjun Ni,Koichi Iwata,Tatsuo Shirota,Masamichi ShinodaPubMed 37946211
- Frontiers in immunology 14:12294142023Distribution of macrophages in the developing cochlea of the common marmoset, a primate model animal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Hosoya,Tsubasa Kitama,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hideyuki Okano,Hiroyuki OzawaPubMed 37675123
- Frontiers in cellular neuroscience 17:12013172023Nicotinamide enhances myelin production after demyelination through reduction of astrogliosis and microgliosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanos Ioannis Kaplanis,Despoina Kaffe,Niki Ktena,Andriani Lygeraki,Ourania Kolliniati,Maria Savvaki,Domna KaragogeosPubMed 37663127
- Brain : a journal of neurology 146:1697-17132023Inhibition of YAP/TAZ-driven TEAD activity prevents growth of NF2-null schwannoma and meningioma.Applications:IFReactive species:HumanLiyam Laraba,Lily Hillson,Julio Grimm de Guibert,Amy Hewitt,Maisie R Jaques,Tracy T Tang,Leonard Post,Emanuela Ercolano,Ganesha Rai,Shyh-Ming Yang,Daniel J Jagger,Waldemar Woznica,Philip Edwards,Aditya G Shivane,C Oliver Hanemann,David B ParkinsonPubMed 36148553
- PloS one 18:e02829582023Effects of acute low-moderate dose ionizing radiation to human brain organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFoluwasomi A Oyefeso,Gabriela Goldberg,Nana Yaa P S Opoku,Marcelo Vazquez,Antonella Bertucci,Zhong Chen,Charles Wang,Alysson R Muotri,Michael J PecautPubMed 37256873
- The Journal of biological chemistry 299:1048022023Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYidan Xu,Joji Kusuyama,Shion Osana,Satayuki Matsuhashi,Longfei Li,Hiroaki Takada,Hitoshi Inada,Ryoichi NagatomiPubMed 37172727
- Frontiers in cell and developmental biology 11:9969522023A 3D cell culture system for bioengineering human neuromuscular junctions to model ALS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBita Massih,Alexander Veh,Maren Schenke,Simon Mungwa,Bettina Seeger,Bhuvaneish T Selvaraj,Siddharthan Chandran,Peter Reinhardt,Jared Sterneckert,Andreas Hermann,Michael Sendtner,Patrick LüningschrörPubMed 36866276
- Nature communications 14:3442023Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Pei,Li Ma,Junjun Jing,Jifan Feng,Yuan Yuan,Tingwei Guo,Xia Han,Thach-Vu Ho,Jie Lei,Jinzhi He,Mingyi Zhang,Jian-Fu Chen,Yang ChaiPubMed 36670126
- Frontiers in pharmacology 14:10853142023Neuroregeneration of injured peripheral nerve by fraction B of catfish epidermal secretions through the reversal of the apoptotic pathway and DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaiba A Al-Arbeed,Waleed M Renno,Jassim M Al-HassanPubMed 36726586
- Acta neuropathologica communications 10:1892022Hydrogen peroxide induced by nerve injury promotes axon regeneration via connective tissue growth factor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuele Negro,Fabio Lauria,Marco Stazi,Toma Tebaldi,Giorgia D'Este,Marco Pirazzini,Aram Megighian,Francesca Lessi,Chiara M Mazzanti,Gabriele Sales,Chiara Romualdi,Silvia Fillo,Florigio Lista,James N Sleigh,Andrew P Tosolini,Giampietro Schiavo,Gabriella Viero,Michela RigoniPubMed 36567321
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
Preservative: 0.032% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | WB | IHC-FrFl | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Tested | Tested | Tested |
| Cow | Expected | Tested | Expected |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20000 | Notes - |
Species Rat | Dilution info 1/20000 | Notes - |
Species Cow | Dilution info 1/20000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-FrFl
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Cow | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
KIAA0845, NFH, NFH, NEFH, KIAA0845, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Recommended products
Anti-Neurofilament heavy polypeptide antibody ab4680 is a chicken polyclonal antibody that is used in Neurofilament heavy polypeptide western blotting, IHC and immunofluorescence. Suitable for human, mouse and rat samples.
- Tried and trusted by researchers since 2003
Same trusted quality, new lower price
Key facts
- Isotype
IgY
- Host species
Chicken
- Storage buffer
Preservative: 0.032% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purity
IgY fraction
- Concentration
- Purification notes
This is an IgY prep, similar to a mammalian ammonium sulphate IgG prep from serum. It is virtually pure IgY and we estimate that about 5% of material is specific to the immunogen.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
- Biological function summary
This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.
- Pathways
Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.
- Associated diseases and disorders
Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Image from Wirt SE et al, J Cell Biol. 2010 Nov 15;191(4):809-25. Epub 2010 Nov 8, Fig 4. DOI 10.1083/jcb.201003048Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
ab4680 staining Neurofilament heavy polypeptide (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.
Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Mixed neuron/glial cultures stained with a mouse monoclonal antibody to neurofilament subunit NF-L (green) and ab4680, chicken antibody to neurofilament heavy polypeptide. This antibody binds primarily to the phosphorylated axonal forms of NF-H, in contrast to the NF-L antibody which stains both axonal and dendritic/perikaryal neurofilaments. The NF-L antibody therefore reveals a prominent cell body in green, while the surrounding axonal profiles are orange, since the are bound by both NF-L and the chicken NF-H antibody. Blue is a DNA stain.
Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Immunohistological analysis of a rat cerebellum section stained with ab4680 at a dilution 1:5,000 in red, and co-stained with rabbit pAb to GFAP at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.
Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free floating sections were stained.
Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680)
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody (ab4680) at 1/20000 dilution
Lane 1: Rat spinal cord lysate
Lane 2: Mouse spinal cord lysate
Lane 3: Cow spinal cord lysate
Predicted band size: 112 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody (ab4680)
Rat Neurons stained with chicken anti-NF-H (green). The NF-H antibody was used at a dilution of 1/100000 using our standard fixation and staining procedure (in protocol section).
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