AB72996

Anti-Neurofilament heavy polypeptide antibody

Name: Anti-Neurofilament heavy polypeptide antibody (ab72996) | Abcam
Brand: Abcam Limited
SKU: AB72996
Rating: 5 (1 reviews)

(1 Review)

(46 Publications)

Chicken Polyclonal Neurofilament heavy polypeptide antibody. Suitable for WB and reacts with Rat samples. Cited in 46 publications.

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Western blot - Anti-Neurofilament heavy polypeptide antibody (AB72996)

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution

All lanes:

Rat cerebral cortex lysate

Predicted band size: 112 kDa

Observed band size: 200 kDa

false

Key facts

Host species

Chicken

Clonality

Polyclonal

Isotype

IgY

Carrier free

Reacts with

Rat

Applications

applications

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

IgY fraction

Storage buffer

Preservative: 0.065% Sodium azide Constituents: Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

See full target information Neurofilament heavy polypeptide

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:2201-2216 PubMed41044342

2025

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Ionescu,Lior Ankol,Anand Ganapathy Subramaniam,Topaz Altman,Iddo Magen,Yahel Cohen,Yehuda Danino,Tal Gradus-Pery,Yoav Niv,Ori Bar Avi,Danielle Geller,Amjd Ibraheem,Ruilei Cheng,Noam Steinberg,Leenor Alfahel,Pauline Duc,Zeynep Ergul-Ulger,Doruk Arslan,Ersin Tan,Florence Rage,Nilo Riva,Angello Quattrini,Can Ebru Bekircan-Kurt,Adrian Israelson,Amir Dori,Eran Hornstein,Eran Perlson

Skeletal muscle 15:21 PubMed40804743

2025

Growth differentiation factor 10 inhibits fat infiltration in tongue muscles of mice with high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghyun A Kim,Christina Xu,Kyungmin Kim,Xiaoxing Xu,Yufei Du,Hyojung J Choo

Science progress 107:368504241281469 PubMed39314156

2024

Incorporation of decellularized-ECM in graphene-based scaffolds enhances axonal outgrowth and branching in neuro-muscular co-cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Serna,Kirtana Sandepudi,Rebecca L Keate,Sophia L Zhang,Kristen Y Cotton,Alberto De La Isla,Matias Murillo,Yasmine Bouricha,Andrea A Domenighetti,Colin K Franz,Sumanas W Jordan

Life science alliance 7: PubMed39242155

2024

Mast cell extracellular trap formation underlies vascular and neural injury and hyperalgesia in sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Donovan A Argueta,Huy Tran,Yugal Goel,Aithanh Nguyen,Julia Nguyen,Stacy B Kiven,Chunsheng Chen,Fuad Abdulla,Gregory M Vercellotti,John D Belcher,Kalpna Gupta

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309617 PubMed38889308

2024

Integration of Functional Human Auditory Neural Circuits Based on a 3D Carbon Nanotube System.

Applications

Unspecified application

Species

Unspecified reactive species

Yiyun Lou,Jiaoyao Ma,Yangnan Hu,Xiaoying Yao,Yaoqian Liu,Mingxuan Wu,Gaogan Jia,Yan Chen,Renjie Chai,Mingyu Xia,Wenyan Li

Science advances 9:eadi0286 PubMed37506203

2023

PTBP1 regulates injury responses and sensory pathways in adult peripheral neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Alber,Pierluigi Di Matteo,Matthew D Zdradzinski,Irene Dalla Costa,Katalin F Medzihradszky,Riki Kawaguchi,Agostina Di Pizio,Philip Freund,Nicolas Panayotis,Letizia Marvaldi,Ella Doron-Mandel,Nataliya Okladnikov,Ida Rishal,Reinat Nevo,Giovanni Coppola,Seung Joon Lee,Pabitra K Sahoo,Alma L Burlingame,Jeffery L Twiss,Mike Fainzilber

Neurobiology of disease 182:106148 PubMed37164288

2023

Impaired motor unit recovery and maintenance in a knock-in mouse model of ALS-associated Kif5a variant.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly A Rich,Megan G Pino,Mehmet E Yalvac,Ashley Fox,Hallie Harris,Maria H H Balch,W David Arnold,Stephen J Kolb

Journal of pharmaceutical analysis 13:590-602 PubMed37440906

2023

Inhibition of histone methyltransferase PRMT5 attenuates cisplatin-induced hearing loss through the PI3K/Akt-mediated mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Zheng,Benyu Nan,Chang Liu,Dongmei Tang,Wen Li,Liping Zhao,Guohui Nie,Yingzi He

Journal of cell science 136: PubMed36218033

2022

A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensing.

Applications

Unspecified application

Species

Unspecified reactive species

Agostina Di Pizio,Letizia Marvaldi,Marie-Christine Birling,Nataliya Okladnikov,Luc Dupuis,Mike Fainzilber,Ida Rishal

Molecular & cellular proteomics : MCP 21:100418 PubMed36180036

2022

A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes.

Applications

Unspecified application

Species

Unspecified reactive species

Didi-Andreas Song,Stefanie Alber,Ella Doron-Mandel,Vera Schmid,Christin A Albus,Orith Leitner,Hedva Hamawi,Juan A Oses-Prieto,Nili Dezorella,Alma L Burlingame,Mike Fainzilber,Ida Rishal

View all publications

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Product promise

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Anti-Neurofilament heavy polypeptide antibody

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB72996)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (46)

Product promise