Anti-Neurofilament heavy polypeptide antibody

• IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1 : 2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1 : 5,000 in green. The blue is DAPI staining of nuclear DNA.

Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

• WB

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB8135)

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Rat spinal cord tissue lysate

Lane 4:

Mouse brain tissue lysate

Lane 5:

Mouse spinal cord tissue lysate

Predicted band size: 112 kDa

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• IHC

CiteAb

Immunohistochemistry - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Immunohistochemistry using Anti-Neurofilament heavy polypeptide antibody, ab8135. Publication image from Chen, O. et al., 2023, J Clin Invest, 36520531. Legend direct from paper.

CTCL is associated with nerve innervations in mouse lymphoma.(A) Double immunostaining for CGRP and β–tubulin III in the back skin with lymphoma from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale bar : 25 µm. (B) Immunostaining for NF200 in the tumor from a CTCL mouse at day 20. Dotted lines show the epidermis. Scale bar : 25 µm. (C) 3D reconstruction of innervated nerves in a β–tubulin III–labeled tumor from a CTCL mouse at day 20. Scale bar : 300 µm. (D) High-magnification image of the boxed area in C. Scale bar : 150 µm.

• IHC

CiteAb

Immunohistochemistry - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Immunohistochemistry using Anti-Neurofilament heavy polypeptide antibody, ab8135. Publication image from Chen, O. et al., 2023, J Clin Invest, 36520531. Legend direct from paper.

CTCL results in nerve injury in the late phases.(A) Double immunostaining for ATF3 and IB4 in cervical DRGs of naive and CTCL mice at day 60. Scale bar : 25 µm. (B) Double immunostaining of ATF3 and the neuronal markers NF200, P2X3, and CGRP in cervical DRGs from CTCL mice at day 60. Scale bar : 25 µm. (C) Percentage of ATF3+, IB4+, NF200+, P2X3+, and CGRP+ neurons in the cervical DRG of CTCL mice at different time points. n = 3. Two-way ANOVA, F(12,30) = 9.15, P < 0.0001. (D) Percentage of ATF3 colocalization with P2X3+ neurons in the cervical DRG of CTCL mice at different time points. n = 3. One-way ANOVA, F(3,8) = 31.89, P < 0.0001. (E) Pie chart showing ATF3 colocalization with NF200+, P2X3+, and CGRP+ neurons in the cervical DRG from a CTCL mouse on day 60. Data were collected from 3 animals. (F) Double immunostaining for CGRP and IB4 in the cervical spinal cord dorsal horn from CTCL mice at day 60. Arrowheads indicate the loss of IB4+ primary afferents in the IIi. Scale bar : 100 µm. Data are expressed as the mean ± SEM. One- or 2-way ANOVA with Bonferroni’s post hoc test, **P < 0.01, ***P < 0.001, and ****P < 0.0001 versus the naive group.