AB8135

Anti-Neurofilament heavy polypeptide antibody

Name: Anti-Neurofilament heavy polypeptide antibody (ab8135) | Abcam
Brand: Abcam Limited
SKU: AB8135
Price: 520 USD
Availability: InStock
Rating: 5 (25 reviews)

(25 Reviews)

(231 Publications)

Anti-Neurofilament heavy polypeptide antibody (ab8135) is a rabbit polyclonal antibody detecting Neurofilament heavy polypeptide in Western Blot, IHC-Fr. Suitable for Mouse, Rat.

- Over 160 publications
- Trusted since 2001

View Alternative Names

Kiaa0845, Nfh, Nefh, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

2 Images

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB8135)

IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1 : 2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1 : 5,000 in green. The blue is DAPI staining of nuclear DNA.

Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB8135)

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Rat spinal cord tissue lysate

Lane 4:

Mouse brain tissue lysate

Lane 5:

Mouse spinal cord tissue lysate

Predicted band size: 112 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-FrFl

applications

Specificity

The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Neurofilament heavy polypeptide antibody (ab8135) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-Fr) in Mouse, Rat samples.

Trusted by the scientific community
Anti-Neurofilament heavy polypeptide (ab8135) was first used in a scientific publication in 2001 and has been cited over 160 times in peer-reviewed journals.

Reviewed by scientists
Anti-Neurofilament heavy polypeptide (ab8135) has over 25 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.03% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (PubMed : 22723690).

See full target information Nefh

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Publications (231)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8077 PubMed40883260

2025

UBE3A promotes foam cell formation and counters remyelination by targeting ABCA1 for proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie Loix,Sam Vanherle,Laura Bolkaerts,Sanne G S Verberk,Mattijs Punt,Flore Wouters,Brecht Moonen,Rob Verhagen,Suzanne A E Van Wouw,Aldo Jongejan,Ben Distel,Ype Elgersma,Mansour Haidar,Noam Zelcer,Jerome J A Hendriks,Jeroen F J Bogie

The Journal of clinical investigation 135: PubMed40875460

2025

Peripherally administered androgen receptor-targeted antisense oligonucleotide rescues spinal pathology in a murine SBMA model.

Applications

Unspecified application

Species

Unspecified reactive species

Changwoo Lee,Zhigang Yu,Curtis J Kuo,Leon Tejwani,Rosalie M Grijalva,Eunwoo Bae,Hien T Zhao,Janghoo Lim,Andrew P Lieberman

Theranostics 15:8176-8201 PubMed40860154

2025

An unrecognized mechanism of self-protection in degenerating neurons mediated by astrocytic YAP through Wnts/β-catenin/EAAT2 signaling in C9orf72-poly-GA mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Li,Yan Wei,Rui Yang,Xuan Luo,Yanzhu Liu,Weiqiao Zhao,Hui Yang,Yumin Wu,Ying Wang,Zhihui Huang

Advanced healthcare materials 14:e04817 PubMed40702833

2025

3D-Printed Scaffolds Promote Enhanced Spinal Organoid Formation for Use in Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Guebum Han,Nicolas S Lavoie,Nandadevi Patil,Olivia G Korenfeld,Hyunjun Kim,Manuel Esguerra,Daeha Joung,Michael C McAlpine,Ann M Parr

Materials today. Bio 33:102036 PubMed40688662

2025

Curcumin-loaded milk-derived sEVs fused with platelet membrane attenuate endothelial senescence and promote spinal cord injury recovery in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaozhi He,Siyuan Zou,Jiawei Wang,Wenbin Zhang,Sheng Lu,Mengxian Jia,Yumin Wu,Xiaowu Lin,Ziwei Fan,Qishun Liang,Yizhe Sheng,Qichuan Zhuge,Bi Chen,Minyu Zhu,Honglin Teng

Scientific reports 15:24794 PubMed40634438

2025

Transcriptomic and electrophysiological alterations underlying phenotypic variability in SCN1A-associated febrile seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Scalise,Alessandro Gaeta,Elio Aprigliano,Valeria Lucchino,Raffaele Covello,Mariagrazia Talarico,Barbara Puccio,Pietro Hiram Guzzi,Pierangelo Veltri,Antonio Gambardella,Lilian Juliana Lissner,Alessandra Morano,Pierangelo Cifelli,Veronica Alfano,Eleonora Palma,Gerardo Perozziello,Gabriele Ruffolo,Giovanni Cuda,Elvira Immacolata Parrotta

Materials today. Bio 32:101895 PubMed40502366

2025

Curcumin-loaded polydopamine nanoparticles-based antioxidant scaffold promote spinal cord repair though dural-regulation of macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshui Mao,Liangliang Chen,Genying Zhuang,Zhiqiang Jin,Shoukun Wu,Mingshuai Ying,Lingchao Sheng,Jianwei Lv,Qiuchen Bi,Jingjia Ye

Biotechnology journal 20:e70047 PubMed40491005

2025

Tensile Forces and Nanofiber Alignment Influence Both Innervated and Non-Innervated Skeletal Myofiber Formation in Custom Mechanobioreactors.

Applications

Unspecified application

Species

Unspecified reactive species

Melanie C Hilman,Foteini Mourkioti,Suradip Das,D Kacy Cullen

Stem cell research & therapy 16:256 PubMed40405285

2025

Autophagy modulation by hADSCs and green light therapy alleviates inflammation and promotes functional recovery after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Chen,Quanxin He,Huan Xie,Bin Gu,Liyi Zhou,Daoyuan Jiang,Hongxin Xie,Li Liang,Zhilai Zhou,Hui Zhang

CNS neuroscience & therapeutics 31:e70440 PubMed40387519

2025

ALOX15-Mediated Neuron Ferroptosis Was Involved in Diabetic Peripheral Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiye Feng,Fuye Li,Zhiqiang Lin,Jian Liu,Xi Chen,Wenxu Yan,Zhongjie Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament heavy polypeptide antibody

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Western blot - Anti-Neurofilament heavy polypeptide antibody (AB8135)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (231)

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