Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img84602.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img145047.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img92584.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32592.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)")
Publications
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- Environmental science and pollution research international 30:88685-887032023Bee venom (Apis mellifera L.) rescues zinc oxide nanoparticles induced neurobehavioral and neurotoxic impact via controlling neurofilament and GAP-43 in rat brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaglaa Z H Eleiwa,Mahmoud Abo-Alkasem Ali,Enas N Said,Mohamed M M Metwally,Yasmina M Abd-ElHakimPubMed 37442924
- Investigative ophthalmology & visual science 64:72023Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaxing Zhou,Sen Lin,Qiumei Hu,Xue Li,Xi Chen,Linlin Luo,Shiyang Ye,Wei Liu,Jian YePubMed 37145604
- Drug design, development and therapy 15:3443-34502021Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuyu Huang,Haoran Yu,Min Liang,Shule Hou,Junmin Chen,Fan Zhang,Xiayu Sun,Huan Jia,Jun YangPubMed 34413631
- Neural regeneration research 15:2306-23172020Human adipose tissue- and umbilical cord-derived stem cells: which is a better alternative to treat spinal cord injury?Applications:Unspecified applicationReactive species:Unspecified reactive speciesAi-Mei Liu,Bo-Li Chen,Ling-Tai Yu,Tao Liu,Ling-Ling Shi,Pan-Pan Yu,Yi-Bo Qu,Kwok-Fai So,Li-Bing ZhouPubMed 32594054
- The Journal of comparative neurology 528:1644-16592020Spatial models of cell distribution in human lumbar dorsal root ganglia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZachariah J Sperry et. al.PubMed 31872433
- Pain 161:532-5442019Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingzheng Wu,Zehua Li,Lei Liang,Pingchuan Ma,Dong Cui,Peng Chen,Genhao Wu,Xue-Jun SongPubMed 31738230
- International endodontic journal 52:211-2222018Evidence for changing nerve growth factor signalling mechanisms during development, maturation and ageing in the rat molar pulp.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Mahdee,J Eastham,J M Whitworth,J I GillespiePubMed 30099752
- Schizophrenia research 177:88-972016Altered CSNK1E, FABP4 and NEFH protein levels in the dorsolateral prefrontal cortex in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaquel Pinacho et. al.PubMed 27236410
- Neuroscience 212:201-132012Neurodegeneration and early lethality in superoxide dismutase 2-deficient mice: a comprehensive analysis of the central and peripheral nervous systems.
- Investigative ophthalmology & visual science 52:8025-322011In vivo serial imaging of regenerating corneal nerves after surgical transection in transgenic thy1-YFP mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbed Namavari et. al.PubMed 21896845
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/8000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
KIAA0845, NFH, NFH, KIAA0845, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Recommended products
Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EP673Y
- Purification technique
Affinity purification Protein A
- Specificity
ab40796 recognises Neuofilaments (NF).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
- Biological function summary
This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.
- Pathways
Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.
- Associated diseases and disorders
Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134825.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling
Neurofilament heavy polypeptide with unpurified ab40796.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img84602.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution
All lanes: Human brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img145047.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--western-blot-img92584.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution
All lanes: Rat brain tissue lysate
Predicted band size: 112 kDa
Observed band size: 200 kDa, 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-ab40796--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32592.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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