AB40796

Anti-Neurofilament heavy polypeptide antibody [EP673Y]

Name: Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) | Abcam
Brand: Abcam Limited
SKU: AB40796
Price: 395 USD
Availability: InStock
Rating: 2 (3 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling

Neurofilament heavy polypeptide with unpurified ab40796.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution

All lanes:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 112 kDa

Observed band size: 200-220 kDa

false

Lab

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 112 kDa

Observed band size: 200-220 kDa

false

Unknown

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution

All lanes:

Rat brain tissue lysate

Predicted band size: 112 kDa

Observed band size: 200 kDa,75 kDa

false

Carrier free

Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP673Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab40796 recognises Neuofilaments (NF).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Environmental science and pollution research international 30:88685-88703 PubMed37442924

2023

Bee venom (Apis mellifera L.) rescues zinc oxide nanoparticles induced neurobehavioral and neurotoxic impact via controlling neurofilament and GAP-43 in rat brain.

Applications

Unspecified application

Species

Unspecified reactive species

Naglaa Z H Eleiwa,Mahmoud Abo-Alkasem Ali,Enas N Said,Mohamed M M Metwally,Yasmina M Abd-ElHakim

Investigative ophthalmology & visual science 64:7 PubMed37145604

2023

Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Zhou,Sen Lin,Qiumei Hu,Xue Li,Xi Chen,Linlin Luo,Shiyang Ye,Wei Liu,Jian Ye

International journal of molecular sciences 23: PubMed36012122

2022

Valproate Targets Mammalian Gastrulation Impairing Neural Tissue Differentiation and Development of the Placental Source In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Katušić-Bojanac,Milvija Plazibat,Marta Himelreich-Perić,Katarina Eck-Raković,Jure Krasić,Nino Sinčić,Gordana Jurić-Lekić,Davor Ježek,Floriana Bulić-Jakuš

Drug design, development and therapy 15:3443-3450 PubMed34413631

2021

Hearing Protection Outcomes of Analog Electrode Arrays Coated with Different Drug-Eluting Polymer Films Implanted into Guinea Pig Cochleae.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyu Huang,Haoran Yu,Min Liang,Shule Hou,Junmin Chen,Fan Zhang,Xiayu Sun,Huan Jia,Jun Yang

Neural regeneration research 15:2306-2317 PubMed32594054

2020

Human adipose tissue- and umbilical cord-derived stem cells: which is a better alternative to treat spinal cord injury?

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Mei Liu,Bo-Li Chen,Ling-Tai Yu,Tao Liu,Ling-Ling Shi,Pan-Pan Yu,Yi-Bo Qu,Kwok-Fai So,Li-Bing Zhou

The Journal of comparative neurology 528:1644-1659 PubMed31872433

2020

Spatial models of cell distribution in human lumbar dorsal root ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Zachariah J Sperry,Robert D Graham,Nicholas Peck-Dimit,Scott F Lempka,Tim M Bruns

Pain 161:532-544 PubMed31738230

2019

Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzheng Wu,Zehua Li,Lei Liang,Pingchuan Ma,Dong Cui,Peng Chen,Genhao Wu,Xue-Jun Song

International endodontic journal 52:211-222 PubMed30099752

2018

Evidence for changing nerve growth factor signalling mechanisms during development, maturation and ageing in the rat molar pulp.

Applications

Unspecified application

Species

Unspecified reactive species

A Mahdee,J Eastham,J M Whitworth,J I Gillespie

Schizophrenia research 177:88-97 PubMed27236410

2016

Altered CSNK1E, FABP4 and NEFH protein levels in the dorsolateral prefrontal cortex in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Pinacho,Núria Villalmanzo,J Javier Meana,Isidre Ferrer,Adriana Berengueras,Josep M Haro,Judit Villén,Belén Ramos

Neuroscience 212:201-13 PubMed22516022

2012

Neurodegeneration and early lethality in superoxide dismutase 2-deficient mice: a comprehensive analysis of the central and peripheral nervous systems.

Applications

IHC-Fr

Species

Mouse

S S Oh,K A Sullivan,J E Wilkinson,C Backus,J M Hayes,S A Sakowski,E L Feldman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament heavy polypeptide antibody [EP673Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (AB40796)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise