Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img84602.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img145047.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img92584.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32592.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (AB247290)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
1 products for Alternative Version
Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NFH, KIAA0845, NEFH
Recommended products
Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Alternative names
Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NFH, KIAA0845, NEFH
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP673Y
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognises Neuofilaments (NF).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab247290 is the carrier-free version of Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
- Biological function summary
This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.
- Pathways
Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.
- Associated diseases and disorders
Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134825.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling
Neurofilament heavy polypeptide with unpurified Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img84602.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796) at 1/2000 dilution
All lanes: Human brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img145047.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796) at 1/2000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--western-blot-img92584.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796) at 1/2000 dilution
All lanes: Rat brain tissue lysate
Predicted band size: 112 kDa
Observed band size: 200 kDa, 75 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-ab247290--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img32592.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified Anti-Neurofilament heavy polypeptide antibody [EP673Y] ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com