Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 14 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-frozen-sections-img89802.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127047.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)")
![Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunocytochemistry-immunofluorescence-img15540.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img23460.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)")
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img161374.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (AB207176)")
Publications
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- Children (Basel, Switzerland) 10:2023Cerebral Myelination in a Bronchopulmonary Dysplasia Murine Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Chen,Ran Wang,Chao ChenPubMed 37628321
- Fluids and barriers of the CNS 20:12023Tocilizumab promotes repair of spinal cord injury by facilitating the restoration of tight junctions between vascular endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Luo,Fei Yao,Yi Shi,Zhenyu Zhu,Zhaoming Xiao,Xingyu You,Yanchang Liu,Shuisheng Yu,Dasheng Tian,Li Cheng,Meige Zheng,Juehua JingPubMed 36624478
- Inflammation and regeneration 42:442022Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFRβ pathway in spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Yao,Yang Luo,Yan-Chang Liu,Yi-Hao Chen,Yi-Teng Li,Xu-Yang Hu,Xing-Yu You,Shui-Sheng Yu,Zi-Yu Li,Lei Chen,Da-Sheng Tian,Mei-Ge Zheng,Li Cheng,Jue-Hua JingPubMed 36163271
- Journal of neuroinflammation 19:1642022Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Xu,Wanli Xie,Yiqi Feng,Yanting Wang,Xia Li,Jie Liu,Yue Xiong,Yuyao He,Lu Chen,Guoyang Liu,Qingping WuPubMed 35729568
- Journal of neuroinflammation 19:952022SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyu Li,Shuisheng Yu,Yanchang Liu,Xuyang Hu,Yiteng Li,Zhaoming Xiao,Yihao Chen,Dasheng Tian,Xinzhong Xu,Li Cheng,Meige Zheng,Juehua JingPubMed 35429978
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21022742021In Vivo Clonal Analysis Reveals Development Heterogeneity of Oligodendrocyte Precursor Cells Derived from Distinct Germinal Zones.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Liu,Yinhang Jia,Peng Guo,Wenhong Jiang,Ruiliang Bai,Chong LiuPubMed 34396711
- Cell & bioscience 11:972021Semaphorin3A increases M1-like microglia and retinal ganglion cell apoptosis after optic nerve injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiu Yun-Jia,Chen Xi,Zhang Jie-Qiong,Zhu Jing-Yi,Lin Sen,Ye JianPubMed 34039431
- Cell death discovery 7:962021Silencing TAK1 reduces MAPKs-MMP2/9 expression to reduce inflammation-driven neurohistological disruption post spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuai Jiang,Yandan Wu,Shunjie Wu,Suhui Ye,Renyi Kong,Jie Chang,Mingjie Xia,Junping Bao,Xin Peng,Xin Hong,Zhanyang Qian,Haijun LiPubMed 33966042
- CNS neuroscience & therapeutics 27:714-7242021EphB2 knockdown decreases the formation of astroglial-fibrotic scars to promote nerve regeneration after spinal cord injury in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian Wu,Bing Lu,Riyun Yang,Ying Chen,Xue Chen,Yi LiPubMed 33794069
- Frontiers in medicine 8:6434022021Evaluation of the Effectiveness of a Chronic Ocular Hypertension Mouse Model Induced by Intracameral Injection of Cross-Linking Hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjue Chen,Jun Sun,Huan Yu,Ping Huang,Yisheng ZhongPubMed 33829024
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Expected | Tested |
| Mouse | Tested | Tested | Tested | Tested |
| Rat | Tested | Expected | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Rat | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
Alternative names
KIAA0845, NFH, NFH, KIAA0845, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein
Recommended products
Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 14 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20020
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
- Biological function summary
This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.
- Pathways
Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.
- Associated diseases and disorders
Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-frozen-sections-img89802.jpg "Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Positive staining on mouse hippocampus is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127047.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the normal Human cerebrum tissue is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunocytochemistry-immunofluorescence-img15540.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling Neurofilament heavy polypeptide with ab207176 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on Neuro-2a cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img23460.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/2: 3 seconds; Lane 3/4: 1 second.
The MW observed is consistent with the literature: PMID: 25374587; PMID: 144556.
Lanes 1 and 3: Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution
Lanes 2 and 4: Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/2000 dilution
Lane 1: Mouse cerebellum lysate at 10 µg
Lane 2: Mouse brain lysate at 10 µg
Lane 3: Rat cerebellum lysate at 10 µg
Lane 4: Rat brain lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 112 kDa
Observed band size: 200-210 kDa
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img161374.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Blocking/Dilution buffer: 5% NFDM/TBST.
The MW observed is consistent with the literature: PMID: 25374587; PMID: 144556.
All lanes: Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution
All lanes: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 200-210 kDa
Exposure time: 3min
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img24939.jpg "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2: 30 seconds.
Lane 1: Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/1000 dilution
Lane 2: Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176) at 1/2000 dilution
Lane 1: Human midbrain lysate at 10 µg
Lane 2: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg
SecondaryLane 1: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 112 kDa
Observed band size: 200-210 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30920.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on Human cerebellum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33154.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on mouse cerebellum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121652.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on neurons of the rat cerebral cortex is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative control: No staining on human kidney.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-frozen-sections-img120790.jpg "Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Positive staining on rat cerebral cortex is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--western-blot-img24939.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img30920.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33154.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176), expandable thumbnail](https://content.abcam.com/products/images/neurofilament-heavy-polypeptide-antibody-epr20020-ab207176--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121652.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] (ab207176)")
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