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AB325550

Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Neurofilament heavy polypeptide antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

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Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)
  • sELISA

Lab

Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)

Interpolated concentration of native Neurofilament heavy polypeptide was measured at different sample concentrations. Undiluted sample is as follows : SH-SY5Y cell extract (100 ug/mL). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)
  • sELISA

Lab

Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)

Interpolated concentration of native Neurofilament heavy polypeptide was measured at different sample concentrations. Undiluted samples are as follows : normal CSF 100%. Sample dilutions are made in 0.4% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)
  • sELISA

Lab

Sandwich ELISA - Anti-Neurofilament heavy polypeptide antibody [EPR25926-82] - BSA and Azide free (Capture) (AB325550)

Sandwich ELISA with the capture antibody ab325550 dilution at 2 µg/mL and detector antibody ab325551 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25926-82

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab325550 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurofilament heavy polypeptide. The recommended pair for sandwich ELISA is:

Capture: ab325550, Human Neurofilament heavy polypeptide Capture Antibody (unconjugated)

Detector: ab325551, Human Neurofilament heavy polypeptide Detector Antibody (unconjugated)

The reference range value is 156-20000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFH
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com