AB187374

Anti-Neurofilament heavy polypeptide antibody [NF421]

Name: Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374) | Abcam
Brand: Abcam Limited
SKU: AB187374
Price: 535 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-P, ICC, WB and reacts with Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEFH.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

Supplier Data

Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Immunocytochemistry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml (green). Membrane stained with Phalloidin (red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

Supplier Data

Western blot - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Western blot analysis of human brain tissue lysate labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

All lanes:

Western blot - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374) at 1 µg/mL

All lanes:

Human brain tissue lysate

Predicted band size: 112 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NF421

Isotype

IgG1

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEFH.

P12036

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 19:1640590 PubMed41036176

2025

Boldine as a neuroprotective agent against motor neuron degeneration in models of amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos A Toro,Wei Zhao,Patricio Garcia Silva,Daniela Retamal-Santibáñez,Fabiola Rojas,Jiangping Pan,Nicholas Johnson,Yorley Duarte,Christopher P Cardozo,Juan C Sáez,Brigitte van Zundert

Molecular medicine (Cambridge, Mass.) 30:185 PubMed39455931

2024

Skeletal myotubes expressing ALS mutant SOD1 induce pathogenic changes, impair mitochondrial axonal transport, and trigger motoneuron death.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Martínez,Mónica Silva,Sebastián Abarzúa,María Florencia Tevy,Enrique Jaimovich,Martha Constantine-Paton,Fernando J Bustos,Brigitte van Zundert

International journal of molecular sciences 24: PubMed37629005

2023

The ε-Isozyme of Protein Kinase C (PKCε) Is Impaired in ALS Motor Cortex and Its Pulse Activation by Bryostatin-1 Produces Long Term Survival in Degenerating SOD1-G93A Motor Neuron-like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina La Cognata,Agata Grazia D'Amico,Grazia Maugeri,Giovanna Morello,Maria Guarnaccia,Benedetta Magrì,Eleonora Aronica,Daniel L Alkon,Velia D'Agata,Sebastiano Cavallaro

Neuron 110:1656-1670.e12 PubMed35276083

2022

Excessive release of inorganic polyphosphate by ALS/FTD astrocytes causes non-cell-autonomous toxicity to motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Cristian Arredondo,Carolina Cefaliello,Agnieszka Dyrda,Nur Jury,Pablo Martinez,Iván Díaz,Armando Amaro,Helene Tran,Danna Morales,Maria Pertusa,Lorelei Stoica,Elsa Fritz,Daniela Corvalán,Sebastián Abarzúa,Maxs Méndez-Ruette,Paola Fernández,Fabiola Rojas,Meenakshi Sundaram Kumar,Rodrigo Aguilar,Sandra Almeida,Alexandra Weiss,Fernando J Bustos,Fernando González-Nilo,Carolina Otero,Maria Florencia Tevy,Daryl A Bosco,Juan C Sáez,Thilo Kähne,Fen-Biao Gao,James D Berry,Katharine Nicholson,Miguel Sena-Esteves,Rodolfo Madrid,Diego Varela,Martin Montecino,Robert H Brown,Brigitte van Zundert

Aging 13:23603-23619 PubMed34647904

2021

Resveratrol promotes axonal regeneration after spinal cord injury through activating Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zimin Xiang,Shuai Zhang,Xiaodong Yao,Libin Xu,Jianwei Hu,Chenghui Yin,Jianmei Chen,Hao Xu

Journal of anatomy 238:36-52 PubMed32783212

2020

Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona C Shenton,Thomas Campbell,James F X Jones,Susan Pyner

Frontiers in cell and developmental biology 8:100 PubMed32211401

2020

Effect of a Bone Marrow-Derived Extracellular Matrix on Cell Adhesion and Neural Induction of Dental Pulp Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Samuele Laudani,Valentina La Cognata,Rosario Iemmolo,Gabriele Bonaventura,Giusy Villaggio,Salvatore Saccone,Maria Luisa Barcellona,Sebastiano Cavallaro,Fulvia Sinatra

Neurotoxicology 67:84-93 PubMed29698629

2018

Ag-NPs induce apoptosis, mitochondrial damages and MT3/OSGIN2 expression changes in an in vitro model of human dental-pulp-stem-cells-derived neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele Bonaventura,Valentina La Cognata,Rosario Iemmolo,Massimo Zimbone,Annalinda Contino,Giuseppe Maccarrone,Bruno Failla,Maria Luisa Barcellona,Francesca Luisa Conforti,Velia D'Agata,Sebastiano Cavallaro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Neurofilament heavy polypeptide antibody [NF421]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Western blot - Anti-Neurofilament heavy polypeptide antibody [NF421] (AB187374)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise