AB3966

Anti-Neurofilament heavy polypeptide antibody [RNF402]

Name: Anti-Neurofilament heavy polypeptide antibody [RNF402] (ab3966) | Abcam
Brand: Abcam Limited
SKU: AB3966
Price: 500 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(10 Publications)

Mouse Monoclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-Fr and reacts with Rat samples. Cited in 10 publications. Immunogen corresponding to Tissue preparation containing NEFH protein.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

1 Images

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (AB3966)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (AB3966)

Immunohistochemistry on frozen section of rat brain labeling Neurofilament heavy polypeptide with ab3966.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RNF402

Isotype

IgM

Carrier free

Reacts with

Rat

Applications

IHC-Fr

applications

Immunogen

Tissue preparation containing NEFH protein. The exact immunogen used to generate this antibody is proprietary information.

P12036

Specificity

Axonal Marker. This antibody reacts with the intermediate filament protein of central and periferal nerve cells. In Western blotting this antibody is reactive with the Neurofilament heavy polypeptide protein only. This antibody reacts with both the phosphorylated and non-phosphorylated isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/10 - 1/100", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 9:rbac038 PubMed35801010

2022

Three-dimensional bioprinting sodium alginate/gelatin scaffold combined with neural stem cells and oligodendrocytes markedly promoting nerve regeneration after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liu,Hui Yang,Dong Chen,Yuanyuan Xie,ChenXu Tai,Liudi Wang,Peng Wang,Bin Wang

Cellular & molecular biology letters 27:27 PubMed35300585

2022

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyun Gao,Feifei Huang,Wenqing Wang,Yuanyuan Xie,Bin Wang

Stem cell research & therapy 12:468 PubMed34419172

2021

Engineered basic fibroblast growth factor-overexpressing human umbilical cord-derived mesenchymal stem cells improve the proliferation and neuronal differentiation of endogenous neural stem cells and functional recovery of spinal cord injury by activating the PI3K-Akt-GSK-3β signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Huang,Tianyun Gao,Wenqing Wang,Liudi Wang,Yuanyuan Xie,Chenxun Tai,Shuo Liu,Yi Cui,Bin Wang

Neural regeneration research 16:2284-2292 PubMed33818514

2021

A multi-channel collagen scaffold loaded with neural stem cells for the repair of spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Liu,Yuan-Yuan Xie,Liu-Di Wang,Chen-Xu Tai,Dong Chen,Dan Mu,Yan-Yan Cui,Bin Wang

Journal of materials science. Materials in medicine 31:115 PubMed33247423

2020

Effects of oxygen generating scaffolds on cell survival and functional recovery following acute spinal cord injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Liangle Liu,Junming Wan,Minghai Dai,Xiuzhi Ye,Chun Liu,Chengxuan Tang,Lixin Zhu

BioMed research international 2020:5046832 PubMed31998792

2020

Nerve Repair and Orthodromic and Antidromic Nerve Grafts: An Experimental Comparative Study in Rabbit.

Applications

Unspecified application

Species

Unspecified reactive species

Jihyeung Kim,Young Eun Choi,Jeong Hwan Kim,Seung Hak Lee,Sohee Oh,Sae Hoon Kim

Science China. Life sciences 61:2-13 PubMed28527111

2017

Human placenta-derived mesenchymal stem cells loaded on linear ordered collagen scaffold improves functional recovery after completely transected spinal cord injury in canine.

Applications

Unspecified application

Species

Unspecified reactive species

Sufang Han,Zhifeng Xiao,Xing Li,Huan Zhao,Bin Wang,Zhixue Qiu,Zhi Li,Xin Mei,Bai Xu,Caixia Fan,Bing Chen,Jin Han,Yanzheng Gu,Huilin Yang,Qin Shi,Jianwu Dai

Science China. Life sciences 59:647-55 PubMed27333785

2016

One-year clinical study of NeuroRegen scaffold implantation following scar resection in complete chronic spinal cord injury patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifeng Xiao,Fengwu Tang,Jiaguang Tang,Huilin Yang,Yannan Zhao,Bing Chen,Sufang Han,Nuo Wang,Xing Li,Shixiang Cheng,Guang Han,Changyu Zhao,Xiaoxiong Yang,Yumei Chen,Qin Shi,Shuxun Hou,Sai Zhang,Jianwu Dai

Spinal cord 52:867-73 PubMed25311846

2014

The collagen scaffold with collagen binding BDNF enhances functional recovery by facilitating peripheral nerve infiltrating and ingrowth in canine complete spinal cord transection.

Applications

Unspecified application

Species

Unspecified reactive species

S Han,B Wang,W Jin,Z Xiao,B Chen,H Xiao,W Ding,J Cao,F Ma,X Li,B Yuan,T Zhu,X Hou,J Wang,J Kong,W Liang,J Dai

The spine journal : official journal of the North 14:1280-90 PubMed24295797

2013

Experimental chemonucleolysis with recombinant human matrix metalloproteinase 7 in human herniated discs and dogs.

Applications

Unspecified application

Species

Human

Hirotaka Haro,Miyuki Nishiga,Daisuke Ishii,Takasumi Shimomoto,Tsuyoshi Kato,Osami Takenouchi,Satoshi Koyanagi,Tetsuro Ohba,Hiromichi Komori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament heavy polypeptide antibody [RNF402]

Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [RNF402] (AB3966)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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