AB8972

Anti-Neurofilament heavy polypeptide antibody [RNF404]

Name: Anti-Neurofilament heavy polypeptide antibody [RNF404] (ab8972) | Abcam
Brand: Abcam Limited
SKU: AB8972

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(3 Publications)

Mouse Monoclonal Neurofilament heavy polypeptide antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Tissue preparation containing Neurofilament heavy polypeptide protein.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RNF404] (AB8972)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RNF404] (AB8972)

Immunohistochemistry on paraffin section of human heart

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RNF404] (AB8972)

mmunofluorescence staining of rat brain with ab8972 (1 : 100).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RNF404

Isotype

IgG2a

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with the intermediate filament protein of periferal and central nerve cells, corresponding in Western blotting with the 200 kDa protein. Specific for the phosphorylated isoform.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.

Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFH

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 18:176-182 PubMed35799539

2022

Diffusion tensor imaging reveals brain structure changes in dogs after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Bin Liu,De-Gang Yang,Jun Li,Chuan Qin,Xin Zhang,Jun Liu,Da-Peng Li,Jian-Jun Li

Toxicologic pathology 47:528-541 PubMed31064296

2019

A Transient Developmental Background Finding in the Retina Observed in Neonatal Dogs in Juvenile Toxicology Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Janet M Petruska,Amera K Remick,Typhaine Lejeune,Mark Vezina,Keith Robinson,Martin Bussières,Brian C Gilger,Richard R Dubielzig

The Journal of biological chemistry 293:11971-11983 PubMed29871925

2018

The CCCH-type zinc finger transcription factor Zc3h8 represses NF-κB-mediated inflammation in digestive organs in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Qingliang Zou,Kai Gang,Qifen Yang,Xiaolin Liu,Xuemei Tang,Huiqiang Lu,Jianbo He,Lingfei Luo

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Neurofilament heavy polypeptide antibody [RNF404]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RNF404] (AB8972)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [RNF404] (AB8972)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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