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AB315203

Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12]

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Mouse Monoclonal Neurofilament heavy polypeptide phospho S503 + S526 + S532 + S540 + S546 + S552 + S560 + S566 + S574 + S580 + S586 + S594 + S600 + S606 + S614 + S620 + S628 + S634 + S640 + S648 + S654 + S660 + S668 + S674 + S682 + S688 + S696 + S702 + S710 + S724 + S730 + S744 + S752 + S758 + S769 + S793 + S801 + S828 antibody. Suitable for WB, IHC-P and reacts with Cow, Rat, Mouse samples. Immunogen corresponding to Native Full Length Protein corresponding to Human NEFH pS503 + S526 + S532 + S540 + S546 + S552 + S560 + S566 + S574 + S580 + S586 + S594 + S600 + S606 + S614 + S620 + S628 + S634 + S640 + S648 + S654 + S660 + S668 + S674 + S682 + S688 + S696 + S702 + S710 + S724 + S730 + S744 + S752 + S758 + S769 + S793 + S801 + S828.

View Alternative Names

KIAA0845, NFH, NEFH, Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)

Immunohistochemical analysis of human cerebellum tissue with ab315203 at 1/5000 dilution, detected with DAB (brown). Hematoxylin (blue) was used as the counterstain. In this image, ab315203 labels Purkinje cell dendrites and the projections of neuronal cells within the granular layer.

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)

Immunofluorescent analysis of rat cortex grown in culture and stained ab315203 at 1/5000 dilution (red) and polyclonal antibody to neurofilament NF-L ab9035 at 1/5000 dilution (green). ab315203 binds to phosphorylated NF-H found in some mature axonal profiles, while the NF-L binds to both axonal, dendritic and developing neuronal processes. The mature axonal profiles contain both NF-L and phosphorylated NF-H and so appear yellow, while processes expressing only NF-L appear green. Nuclear DNA is revealed by DAPI dye in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide (phospho S) antibody [9B12] (AB315203)

Immunohistological analysis of a rat brain coronal section of the third ventricle stained with ab315203, dilution 1 : 5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μm, and free-floating sections were stained with above antibody. The ab315203 antibody is a robust marker of the axons of neuronal cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9B12

Isotype

IgG2b

Carrier free

No

Reacts with

Rat, Mouse, Cow

Applications

WB, IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human NEFH pS503 + S526 + S532 + S540 + S546 + S552 + S560 + S566 + S574 + S580 + S586 + S594 + S600 + S606 + S614 + S620 + S628 + S634 + S640 + S648 + S654 + S660 + S668 + S674 + S682 + S688 + S696 + S702 + S710 + S724 + S730 + S744 + S752 + S758 + S769 + S793 + S801 + S828.

P12036

Specificity

The predominant type of NF-H is the axonal form which is heavily serine phosphorylated on 40 or more tandemly repeated lysine-serine-proline (KSP) containing peptides.

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Reactivity data

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurofilament heavy polypeptide often referred to as NF-H or neurofilament heavy chain plays an important role in the structural integrity of neurons. This protein is a critical component of the cytoskeleton in neurons consisting of an approximate molecular mass of 200 kDa. It is predominantly expressed in the neuronal axons where it contributes to the assembly and stability of neurofilament structures. NF-H is essential for maintaining axonal diameter and for proper conduction of electrical impulses along nerve fibers.
Biological function summary

This polypeptide participates in the formation of neurofilament a complex structure that includes other neurofilament proteins like the neurofilament light (NF-L) and neurofilament medium (NF-M). These proteins form a stable scaffold within the axon aiding in neuronal growth and repair. The phosphorylation of NF-H modulates its interactions within the neurofilament network influencing axonal transport processes and dynamics which are vital for neuron function and survival.

Pathways

Neurofilament heavy polypeptide is integral to the axonal transport pathway important in conveying molecules between the neuronal cell body and the synaptic terminals. Through its interactions with microtubules and motor proteins NF-H facilitates the movement of organelles and neurotransmitters. It has significant associations with proteins involved in the ubiquitin-proteasome system reflecting its role in maintaining neuronal homeostasis and proteostasis essential in neuronal development and function.

Neurofilament heavy polypeptide serves as an important biomarker for neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS) and Alzheimer's disease. NF-H especially in phosphorylated form accumulates in conditions of axonal injury or degeneration. It often interacts with tau protein in Alzheimer's pathology forming neurofibrillary tangles. Elevated levels of NF-H in cerebrospinal fluid or blood can indicate neuronal damage offering insights into disease progression and potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
See full target information NEFH pS503 + S526 + S532 + S540 + S546 + S552 + S560 + S566 + S574 + S580 + S586 + S594 + S600 + S606 + S614 + S620 + S628 + S634 + S640 + S648 + S654 + S660 + S668 + S674 + S682 + S688 + S696 + S702 + S710 + S724 + S730 + S744 + S752 + S758 + S769 + S793 + S801 + S828
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Product promise

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