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Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEFL aa 150-200.

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Images

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079), expandable thumbnail
  • Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079), expandable thumbnail
  • Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human NEFL aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07196

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Tested

Tested
Tested

Species
Human, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

Alternative names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

Recommended products

Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEFL aa 150-200.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human NEFL aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07196
Clone number
BLR224K
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Associated diseases and disorders

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Samples: Whole cell lysate (25 µg) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

    All lanes: Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

    Lane 1: HEK293T Whole cell lysate at 25 µg

    Lane 2: HeLa Whole cell lysate at 25 µg

    Lane 3: SK-N-BE(2) Whole cell lysate at 25 µg

    Lane 4: Jurkat Whole cell lysate at 25 µg

    Lane 5: 786-O Whole cell lysate at 25 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds.

    All lanes: Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

    Lane 1: NIH 3T3 Whole cell lysate at 50 µg

    Lane 2: CT26 Whole cell lysate at 50 µg

    Lane 3: B35 Whole cell lysate at 50 µg

    Lane 4: TCMK-1 Whole cell lysate at 50 µg

    Lane 5: BW5147.3 Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR221K] (A700-224) used for IP at 20 µl/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, A700-224 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Sample: FFPE section of mouse cerebellum. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary: HRP-conjugated goat antirabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Sample: FFPE section of human medulla. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary: HRP-conjugated goat antirabbit IgG.

  • Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Sample: FFPE section of B35 cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224). Secondary: HRP-conjugated goat anti-rabbit IgG.

  • Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079), expandable thumbnail

    Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079)

    Sample: FFPE section of SK-N-BE(2) cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary: HRP-conjugated goat anti-rabbit IgG.

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