AB314079

Anti-Neurofilament-L antibody [BLR224K] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal 68kDa Neurofilament/NF-L antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human NEFL aa 150-200.

View Alternative Names

NF68, NFL, NEFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein

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Supplier Data

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of SK-N-BE(2) cells. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of human medulla. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR221K] (A700-224) used for IP at 20 µl/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, A700-224 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 30 seconds.

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of mouse cerebellum. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Sample : FFPE section of B35 cells. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Samples : Whole cell lysate (25 µg) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

Lane 1:

HEK293T Whole cell lysate at 25 µg

Lane 2:

HeLa Whole cell lysate at 25 µg

Lane 3:

SK-N-BE(2) Whole cell lysate at 25 µg

Lane 4:

Jurkat Whole cell lysate at 25 µg

Lane 5:

786-O Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Samples : Whole cell lysate (50 µg) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-Neurofilament-L recombinant monoclonal antibody [BLR224K] (A700-224CF) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (ab314079) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 50 µg

Lane 2:

CT26 Whole cell lysate at 50 µg

Lane 3:

B35 Whole cell lysate at 50 µg

Lane 4:

TCMK-1 Whole cell lysate at 50 µg

Lane 5:

BW5147.3 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR224K

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-P, ICC, WB, IP

applications

Immunogen

Synthetic Peptide within Human NEFL aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P07196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 68kDa Neurofilament also known as NF-L or neurofilament light plays a mechanical role in maintaining the structural integrity of neurons. This protein is characterized by its mass of 68 kilodaltons. It expresses specifically in the cytoplasm of neurons where it forms a critical part of the neurofilament protein triplet along with NF-M and NF-H. These components collectively form the intermediate filaments found in the neuron axons providing essential structural support.

Biological function summary

The neurofilament protein functions to stabilize the axonal diameter thereby affecting neuronal conductivity. It integrates into a complex with other neurofilament proteins and related molecules forming cross-bridges that aid in strengthening the cellular structure. This protein-protein interaction is vital for normal neuronal function and efficient nerve signal transmission.

Pathways

Neurofilament proteins contribute to cellular processes within the nervous system. It participates in the axonal transport pathway which includes the transport of synaptic vesicles and organelles along axons. This movement is facilitated by interactions with motor proteins like kinesin and dynein which help in moving these materials to their destinations. Additionally NF-L connects to actin filaments collaborating to sustain neuronal shape and function.

Neurofilament proteins have links to conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Elevated levels of NF-L are often observed in ALS suggesting neuronal damage as axonal injury raises the protein's presence in the cerebrospinal fluid. Similarly in Alzheimer's disease aberrant interaction of tau protein with neurofilament proteins disrupts neuronal function. These connections highlight NF-L's potential as a biomarker for neural injury and neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFL

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurofilament-L antibody [BLR224K] - BSA free

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Immunoprecipitation - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Immunocytochemistry - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Western blot - Anti-Neurofilament-L antibody [BLR224K] - BSA free (AB314079)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise