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AB272926

Anti-Neurogenin 1/NEUROG1 antibody

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(1 Publication)

Rabbit Polyclonal Neurogenin 1/NEUROG1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Neurog1.

View Alternative Names

BHLHA6, NEUROD3, NGN, NGN1, NEUROG1, Neurogenin-1, NGN-1, Class A basic helix-loop-helix protein 6, Neurogenic basic-helix-loop-helix protein, Neurogenic differentiation factor 3, bHLHa6, NeuroD3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)

Paraffin-embedded E13.5 mouse embryo tissue stained for Neurogenin 1/NEUROG1 using ab272926 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)

Paraffin-embedded E13.5 mouse embryo tissue stained for Neurogenin 1/NEUROG1 using ab272926 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Western blot - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)
  • WB

Unknown

Western blot - Anti-Neurogenin 1/NEUROG1 antibody (AB272926)

12% SDS-PAGE

All lanes:

Western blot - Anti-Neurogenin 1/NEUROG1 antibody (ab272926) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T whole cell extracts at 30 µg

Lane 2:

Neurogenin 1/NEUROG1 transfected HEK-293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Mouse Neurog1. The exact immunogen used to generate this antibody is proprietary information.

P70660

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurogenin 1 (NEUROG1) also known as neurogenin belongs to the family of basic helix-loop-helix (bHLH) transcription factors. The molecular mass of NEUROG1 is approximately 23 kilodaltons. This protein is mainly expressed in developing neural tissues where it plays an important role in the differentiation of neuronal precursor cells into mature neurons. NEUROG1 is strongly expressed in the central nervous system particularly during neurogenesis.
Biological function summary

The gene encoding NEUROG1 serves a critical function in the specification of neuronal identity. It acts as a transcriptional regulator guiding the assembly of cell-specific transcriptional complexes. NEUROG1 does not work alone; it interacts with other bHLH factors such as Mash1 to propagate neuronal differentiation signals. The protein is essential for initiating the cascade of neuronal gene expression programs that lead to the development of the nervous system.

Pathways

NEUROG1 participates in the neurogenic pathway particularly influencing the Notch signaling pathway and the Wnt signaling pathway. Within these pathways NEUROG1 cooperates with proteins like Delta-Notch and Wnt to regulate the balance between proliferation and differentiation in progenitor cells. This ensures proper neuronal patterning and development optimizing the formation and functionality of the nervous system.

NEUROG1 has links to certain neurodevelopmental disorders and neuronal damage. Mutations or misregulation of NEUROG1 can contribute to conditions such as autism spectrum disorder and epileptic syndromes. Its interaction with proteins such as Notch1 is significant as altered Notch signaling is often observed in these conditions highlighting the role of NEUROG1 in maintaining neural homeostasis and signaling integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis (By similarity).
See full target information NEUROG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 237:2877-2887 PubMed35342944

2022

Role of the hydrogen sulfide-releasing donor ADT-OH in the regulation of mammal neural precursor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Wen Wei,Ming-Ming Zou,Jian Huan,Di Li,Peng-Fei Zhang,Mei-Hong Lu,Jian Xiong,Yan-Xia Ma
View all publications
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Product promise

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