Anti-Neurogenin 1/NEUROG1 antibody
1
(1 Review)
|
(5 Publications)
Rabbit Polyclonal Neurogenin 1/NEUROG1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NEUROG1.
View Alternative Names
BHLHA6, NEUROD3, NGN, NGN1, NEUROG1, Neurogenin-1, NGN-1, Class A basic helix-loop-helix protein 6, Neurogenic basic-helix-loop-helix protein, Neurogenic differentiation factor 3, bHLHa6, NeuroD3
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogenin 1/NEUROG1 antibody (AB66498)
ab66498 staining human normal liver. Staining is localised to the nucleus.
Left panel : with primary antibody diluted 1 : 1000. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Neurogenin 1/NEUROG1 antibody (AB66498)
ICC/IF image of ab66498 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66498, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Reactivity data
Properties and storage information
Form
Purity
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The gene encoding NEUROG1 serves a critical function in the specification of neuronal identity. It acts as a transcriptional regulator guiding the assembly of cell-specific transcriptional complexes. NEUROG1 does not work alone; it interacts with other bHLH factors such as Mash1 to propagate neuronal differentiation signals. The protein is essential for initiating the cascade of neuronal gene expression programs that lead to the development of the nervous system.
Pathways
NEUROG1 participates in the neurogenic pathway particularly influencing the Notch signaling pathway and the Wnt signaling pathway. Within these pathways NEUROG1 cooperates with proteins like Delta-Notch and Wnt to regulate the balance between proliferation and differentiation in progenitor cells. This ensures proper neuronal patterning and development optimizing the formation and functionality of the nervous system.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (5)
Recent publications for all applications. Explore the full list and refine your search
Cells 9: PubMed32630355
2020
Applications
Unspecified application
Species
Unspecified reactive species
Environmental toxicology 34:548-555 PubMed30698896
2019
Applications
Unspecified application
Species
Unspecified reactive species
Development (Cambridge, England) 145: PubMed30201687
2018
Applications
Unspecified application
Species
Unspecified reactive species
Molecular medicine reports 14:3595-601 PubMed27573468
2016
Applications
WB
Species
Rat
Nature neuroscience 18:25-35 PubMed25420069
2014
Applications
ICC
Species
Mouse
Complementary Products
Primary Antibodies
AB32454
Anti-MAP2 antibody - Neuronal Marker
BOND RX™ Validated|Lab Essentials
- 5
- 35
Primary Antibodies
AB252881
BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)](https://content.abcam.com/products/images/gfp-antibody-3h9-ab252881--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128926.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com