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AB176124

Anti-Neurogenin3/NGN-3 antibody

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(1 Review)

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(7 Publications)

Rabbit Polyclonal Neurogenin3/NGN-3 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NEUROG3.

View Alternative Names

ATOH5, BHLHA7, NGN3, NEUROG3, Neurogenin-3, NGN-3, Class A basic helix-loop-helix protein 7, Protein atonal homolog 5, bHLHa7

1 Images
Western blot - Anti-Neurogenin3/NGN-3 antibody (AB176124)
  • WB

Supplier Data

Western blot - Anti-Neurogenin3/NGN-3 antibody (AB176124)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-Neurogenin3/NGN-3 antibody (ab176124) at 1/1000 dilution

Lane 1:

HT-29 cell lysate at 25 µg

Lane 2:

293T cell lysate at 25 µg

Lane 3:

H460 cell lysate at 25 µg

Secondary

All lanes:

HRP Goat AntiRabbit IgG (H+L)

Predicted band size: 23 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NEUROG3.

Q9Y4Z2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurogenin3 (NGN-3) also known as NGN3 or NEUROD3 is a basic helix-loop-helix (bHLH) transcription factor with a molecular weight of approximately 22 kDa. Neurogenin3 is important in regulating the commitment of progenitor cells to endocrine pancreas development. It expresses mainly in the pancreas and its expression also extends to parts of the developing nervous system. Researchers often look for these expression patterns to identify cell lineage determination processes.
Biological function summary

Neurogenin3 plays a significant role in the differentiation of endocrine cells within the pancreas. Neurogenin3 functions by binding to specific DNA sequences initiating the transcription of genes necessary for endocrine cell maturation. It does not operate as part of a large complex but acts by recruiting other co-factors to modulate the gene expression directly. Through its transcriptional activity neurogenin3 initiates a cascade of events leading to the differentiation of pancreatic progenitor cells into distinct endocrine lineages such as insulin-producing beta cells.

Pathways

Neurogenin3 integrates into dynamic signaling networks that guide the differentiation process of pancreatic progenitor cells. Notably neurogenin3 is a vital component of the Notch signaling pathway which it helps regulate by activating and repressing genes that determine cell fate. Neurogenin3 interacts with other important proteins such as HES1 and PAX4 within these pathways. The tight balance between these factors and neurogenin3 expression levels determines the progression of progenitor cells into specific cell types.

Neurogenin3 correlates closely with diabetes mellitus and congenital malformations of the pancreas. Mutations in the NGN3 gene result in impaired development of the endocrine pancreas often leading to neonatal diabetes and congenital hyperinsulinism. In connection with diabetic conditions alterations in neurogenin3 expression levels can disrupt normal beta-cell development promoting diabetes onset. Additionally related proteins like NEUROD1 play a role in these disorders suggesting overlapping regulatory mechanisms that emphasize the importance of neurogenin3 in maintaining proper pancreatic function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity).
See full target information NEUROG3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, obesity & metabolism 26:4510-4521 PubMed39072950

2024

Obesity-induced upregulation of miR-483-5p impairs the function and identity of pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Honglei Yuan,Mei He,Qinnan Yang,Fandi Niu,Yuchen Zou,Chen Liu, Yang Yang,Aiming Liu,Xiaoai Chang,Fang Chen,Tijun Wu,Xiao Han,Yaqin Zhang

Nature communications 15:2526 PubMed38514666

2024

Trimethylamine N-oxide impairs β-cell function and glucose tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Kong,Qijin Zhao,Xiaojing Jiang,Jinping Hu,Qian Jiang,Li Sheng,Xiaohong Peng,Shusen Wang,Yibing Chen,Yanjun Wan,Shaocong Hou,Xingfeng Liu,Chunxiao Ma,Yan Li,Li Quan,Liangyi Chen,Bing Cui,Pingping Li

Artificial organs 47:1298-1308 PubMed37032529

2023

Transplantation of insulin-producing cells derived from human MSCs to treat diabetes in a non-human primate model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Zhoujun,Feng Bingzheng,Yang Yuwei,Zhang Yingying,Xu Zhiran,He Chunhua,Leng Jing,Tang Haibo,Li Wanli,Zhang Ting,Li Fujun,Chen Jibing,Gao Hongjun

Cell transplantation 31:9636897221081483 PubMed35236160

2022

The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua Zhang

PloS one 16:e0243340 PubMed34115756

2021

Structural changes in endocrine pancreas of male Wistar rats due to chronic cola drink consumption. Role of PDX-1.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Cao,Julián González,Juan P Ortiz Fragola,Angélica Muller,Mariano Tumarkin,Marisa Moriondo,Francisco Azzato,Manuel Vazquez Blanco,José Milei

Cerebral cortex (New York, N.Y. : 1991) 30:4617-4632 PubMed32219328

2020

TRIM32 Deficiency Impairs Synaptic Plasticity by Excitatory-Inhibitory Imbalance via Notch Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Ntim,Qi-Fa Li,Yue Zhang,Xiao-Da Liu,Na Li,Hai-Lun Sun,Xuan Zhang,Bakhtawar Khan,Bin Wang,Qiong Wu,Xue-Fei Wu,Williams Walana,Khizar Khan,Quan-Hong Ma,Jie Zhao,Shao Li

American journal of physiology. Lung cellular and 312:L669-L677 PubMed28213472

2017

β-Microglobulin participates in development of lung emphysema by inducing lung epithelial cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Na Gao,Ying Wang,Chun-Ming Zheng,Yan-Li Gao,Hui Li,Yan Li,Ting-Ting Fu,Li-Li Xu,Wei Wang,Sun Ying,Kewu Huang
View all publications
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