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AB99269

Anti-Neurogranin antibody

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(3 Publications)

Goat Polyclonal Neurogranin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NRGN aa 1 to C-terminus.

View Alternative Names

Neurogranin, Ng, RC3, NRGN

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody (AB99269)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody (AB99269)

ab99269 (3.8µg/ml) staining of paraffin embedded Human Cortex shows cytoplasm staining in the neuronal cell bodies. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western blot - Anti-Neurogranin antibody (AB99269)
  • WB

Unknown

Western blot - Anti-Neurogranin antibody (AB99269)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Neurogranin antibody (ab99269) at 0.1 µg/mL

All lanes:

Mouse brain lysate in RIPA buffer at 35 µg

Predicted band size: 8 kDa

true

Western blot - Anti-Neurogranin antibody (AB99269)
  • WB

Unknown

Western blot - Anti-Neurogranin antibody (AB99269)

All lanes:

Western blot - Anti-Neurogranin antibody (ab99269) at 2 µg/mL

Lane 1:

Human cerebellum lysates at 35 µg

Lane 2:

Rat brain lysates at 35 µg

Predicted band size: 8 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NRGN aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92686

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.</p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. An approximately 15 kDa band is observed in Human Brain (Cerebral Cortex and Hippocampus), and in Mouse Brain and Rat Brain lysates. There is also an additional 16kDa band. The observed molecular weights correspond to earlier findings in the literature with different antibodies.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab99269 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
Biological function summary

Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

Pathways

Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
See full target information NRGN

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e23711 PubMed38605443

2024

CircBCL2L13 attenuates cardiomyocyte oxidative stress and apoptosis in cardiac ischemia‒reperfusion injury via miR-1246/PEG3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Wu,Hairui Li,Qian Zhang,Jia Song,Yongbin Chen,Ze-Mu Wang,Weipeng Jiang

eLife 11: PubMed36226913

2022

Early life stressful experiences escalate aggressive behavior in adulthood via changes in transthyretin expression and function.

Applications

Unspecified application

Species

Unspecified reactive species

Rohit Singh Rawat,Aksheev Bhambri,Muneesh Pal,Avishek Roy,Suman Jain,Beena Pillai,Arpita Konar

Brain, behavior, and immunity 82:178-187 PubMed31437533

2019

Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Sun,Rui Han,Tong Cheng,Yuhan Zheng,Jia Xiao,Kwok-Fai So,Li Zhang
View all publications

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