Anti-Neurogranin antibody [EPR21152] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic but also weak nuclear staining on human cerebrum (PMID : 26076492; PMID : 21516261) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

This data was developed using ab217672, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain tissue* labelling NRGN with ab217672 at 0.1ug/ml followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining in human Alzheimer's brain.

The section was incubated with ab217672 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0 epitope retrieval solution1) for 20 mins.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on rat cerebrum (PMID : 26076492) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on mouse cerebrum (PMID : 26076492) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized  frozen rat hippocampus CA1 tissue labeling Neurogranin with ab217672 at 1/3000 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on rat brain (PMID : 15389631; 21516261) is observed. Counter stained with DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunocytochemistry/ Immunofluorescence analysis of primary rat cortical neurons labeling Neurogranin with ab217672 at 1/1500. The cells were fixed with 4% paraformaldehyde containing 0.2% picric acid in 0.1M phosphate buffer, pH 6.9 for 20 minutes. Permeabilization was with 0.3% Triton-X 100 in PBS (PBSTx). ab150068 at 1/200 was used as the secondary antibody

The rat cortical neurons were cultured for 29 days in vitro. They were either left untreated (Control) or treated beginning on the 10^th day with 60ng/mL triiodothyronine (T3), to enhance neurogranin expression.

The cells were visualized with an inverted microscope at 10X magnification (upper panels) or 20X magnification (lower panels).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

Image courtesy of Ms. Babben Tinner, QBM Cell Science.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus CA1 tissue labeling Neurogranin with ab217672 at 1/3000 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on mouse brain (PMID : 15389631; 21516261) is observed. Counter stained with DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

• IP

Supplier Data

Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (AB230154)

Neurogranin was immunoprecipitated from 0.35 mg mouse cerebral cortex lysate with ab217672 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217672 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

Lane 1 : Mouse cerebral cortex lysate 10 μg (Input).
Lane 2 : ab217672 IP in mouse cerebral cortex lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab217672 in mouse cerebral cortex lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217672).

All lanes:

Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] (<a href='/en-us/products/primary-antibodies/neurogranin-antibody-epr21152-ab217672'>ab217672</a>)

Predicted band size: 8 kDa

Observed band size: 15 kDa

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