Anti-Neurogranin antibody [EPR21152] - BSA and Azide free

7 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on rat cerebrum (PMID: 26076492) is observed. Counter stained with Hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

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  Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus CA1 tissue labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/3000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on mouse brain (PMID: 15389631; 21516261) is observed. Counter stained with DAPI (blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

  Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

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  Image courtesy of Ms. Babben Tinner, QBM Cell Science.

  Immunocytochemistry/ Immunofluorescence - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunocytochemistry/ Immunofluorescence analysis of primary rat cortical neurons labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/1500. The cells were fixed with 4% paraformaldehyde containing 0.2% picric acid in 0.1M phosphate buffer, pH 6.9 for 20 minutes. Permeabilization was with 0.3% Triton-X 100 in PBS (PBSTx). Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150068 at 1/200 was used as the secondary antibody

  The rat cortical neurons were cultured for 29 days in vitro. They were either left untreated (Control) or treated beginning on the 10^th day with 60ng/mL triiodothyronine (T3), to enhance neurogranin expression.

  The cells were visualized with an inverted microscope at 10X magnification (upper panels) or 20X magnification (lower panels).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

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  Immunohistochemistry (Frozen sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized  frozen rat hippocampus CA1 tissue labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/3000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining in the stratum pyramidal neurons of hippocampus CA1 on rat brain (PMID: 15389631; 21516261) is observed. Counter stained with DAPI (blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

  Perform heat-mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

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  Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Neurogranin was immunoprecipitated from 0.35 mg mouse cerebral cortex lysate with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Neurogranin antibody [EPR21152] ab217672 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5,000 dilution.
  

  Lane 1: Mouse cerebral cortex lysate 10 μg (Input).
  Lane 2: Anti-Neurogranin antibody [EPR21152] ab217672 IP in mouse cerebral cortex lysate (+).
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Neurogranin antibody [EPR21152] ab217672 in mouse cerebral cortex lysate (-).

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 15 seconds.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

  All lanes: Immunoprecipitation - Anti-Neurogranin antibody [EPR21152] (Anti-Neurogranin antibody [EPR21152] ab217672)

  Predicted band size: 8 kDa

  Observed band size: 15 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic staining on mouse cerebrum (PMID: 26076492) is observed. Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [EPR21152] - BSA and Azide free (ab230154)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with Anti-Neurogranin antibody [EPR21152] ab217672 at 1/5000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Mainly cytoplasmic but also weak nuclear staining on human cerebrum (PMID: 26076492; PMID: 21516261) is observed. Counter stained with Hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neurogranin antibody [EPR21152] ab217672).