Anti-Neurogranin antibody [Ng2]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (AB273443)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (AB273443)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] (AB273443)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053μg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

• IP

Lab

Immunoprecipitation - Anti-Neurogranin antibody [Ng2] (AB273443)

Neurogranin was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with ab273443 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273443 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

Lane 1 : Mouse hippocampus tissue lysate 10μg.

Lane 2 : ab273443 IP in mouse hippocampus tissue lysate.

Lane 3 : Mouse monoclonal IgG2b instead of ab273443 in Mouse hippocampus tissue lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 58 seconds.

All lanes:

Immunoprecipitation - Anti-Neurogranin antibody [Ng2] (ab273443)

Predicted band size: 8 kDa

Observed band size: 15 kDa

false

• WB

Lab

Western blot - Anti-Neurogranin antibody [Ng2] (AB273443)

Negative control : Mouse spleen (PMID : 20665622).

The molecular weight observed is consistent with what has been described in the literature ( PMID : 20875798).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 secs; Lanes 2-4 : 3 secs.

All lanes:

Western blot - Anti-Neurogranin antibody [Ng2] (ab273443) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat hippocampus tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 8 kDa

Observed band size: 15 kDa

false