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Mouse Recombinant Monoclonal Neurogranin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Neurogranin antibody [NG22] (AB273444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (AB273444), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (AB273444), expandable thumbnail

Publications

  • Acta neuropathologica 136:363-3762018
    Cerebrospinal fluid neurogranin concentration in neurodegeneration: relation to clinical phenotypes and neuropathology.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Erik Portelius,Bob Olsson,Kina Höglund,Nicholas C Cullen,Hlin Kvartsberg,Ulf Andreasson,Henrik Zetterberg,Åsa Sandelius,Leslie M Shaw,Virginia M Y Lee,David J Irwin,Murray Grossman,Daniel Weintraub,Alice Chen-Plotkin,David A Wolk,Leo McCluskey,Lauren Elman,Jennifer McBride,Jon B Toledo,John Q Trojanowski,Kaj Blennow
    PubMed 29700597

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIHC-FrICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).

    Alternative names

    Neurogranin, Ng, RC3, NRGN

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    Mouse Recombinant Monoclonal Neurogranin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

    Alternative names

    Neurogranin, Ng, RC3, NRGN

    Key facts

    Isotype

    IgG1

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    NG22

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    Supplementary info

    Activity summary

    Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.

    Biological function summary

    Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.

    Pathways

    Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.

    Associated diseases and disorders

    Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    3 product images

    • Western blot - Anti-Neurogranin antibody [NG22] (ab273444), expandable thumbnail

      Western blot - Anti-Neurogranin antibody [NG22] (ab273444)

      Negative control: Human lung (PMID: 20665622).
      The molecular weight observed is consistent with what has been described in the literature (PMID: 20875798).

      Blocking/Dilution buffer: 5% NFDM/TBST.

      All lanes: Western blot - Anti-Neurogranin antibody [NG22] (ab273444) at 1/1000 dilution

      Lane 1: Human brain lysate at 10 µg

      Lane 2: Human lung lysate at 10 µg

      Secondary

      All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Predicted band size: 8 kDa

      Exposure time: 37s

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (ab273444), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (ab273444)

      This data was developed using ab273444 the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with abab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond� Polymer Refine Detection) was used. Positive staining on human cererbum. The section was incubated with ab273444 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond� Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (ab273444), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] (ab273444)

      This data was developed using ab273444 the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Neurogranin with abab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond� Polymer Refine Detection) was used. Negative control: no staining on human lung. The section was incubated with ab273444 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond� Polymer Refine Detection) was used.

      Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com