Anti-Neurogranin antibody [NG22] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] - BSA and Azide free (AB273465)

This data was developed using ab273444 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human cererbum. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [NG22] - BSA and Azide free (AB273465)

This data was developed using ab273444 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Neurogranin with ab273444 at 1/10000 (0.111 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control : No staining on human lung. The section was incubated with ab273444 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• WB

Supplier Data

Western blot - Anti-Neurogranin antibody [NG22] - BSA and Azide free (AB273465)

This data was developed using ab273444 the same antibody clone in a different buffer formulation.

Negative control : Human lung (PMID : 20665622).
The molecular weight observed is consistent with what has been described in the literature (PMID : 20875798).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Neurogranin antibody [NG22] (<a href='/en-us/products/primary-antibodies/neurogranin-antibody-ng22-ab273444'>ab273444</a>) at 1/1000 dilution

Lane 1:

Human brain lysate at 10 µg

Lane 2:

Human lung lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 8 kDa

false

Exposure time: 37s