Mouse Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Neurogranin antibody [NG22] - BSA and Azide free (Capture) (AB275781), expandable thumbnail](https://content.abcam.com/products/images/neurogranin-antibody-ng22-bsa-and-azide-free-capture-ab275781--sandwich-elisa-img46623.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Neurogranin antibody [NG22] - BSA and Azide free (Capture) (AB275781)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
Alternative names
Neurogranin, Ng, RC3, NRGN
Recommended products
Mouse Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- NG22
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab275781 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurogranin. The recommended pair for sandwich ELISA is:
Capture: ab275781, Human Neurogranin Capture Antibody (unconjugated)
Detector: Anti-Neurogranin antibody [Ng2] - BSA and Azide free (Detector) ab275782, Human Neurogranin Detector Antibody (unconjugated)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neurogranin also known as NRGN is a small neuronal protein with an approximate molecular mass of 7.6 kDa. It is mainly expressed in the brain predominantly within the dendrites of neurons. Neurogranin binds to calmodulin in the presence of calcium playing an important role in synaptic plasticity. This protein acts as a kinase substrate influencing signal transduction pathways tied to learning and memory. Due to its specific expression pattern neurogranin serves as a significant molecular marker for studying the nervous system.
- Biological function summary
Neurogranin functions as a modulator in cerebellar signaling and synaptic transmission. It influences calcium-calmodulin signaling facilitating the activation of proteins important in the central nervous system. Neurogranin does not form part of a multi-protein complex but interacts closely with calmodulin to regulate downstream signal cascades. These interactions emphasize its fundamental role in modulating processes that influence cognitive functions like learning and memory.
- Pathways
Neurogranin significantly impacts the calcium signaling pathway and contributes to the regulation of long-term potentiation. It closely associates with proteins such as Protein Kinase C (PKC) and calmodulin-dependent protein kinases which are instrumental in these pathways. By modulating these essential signaling routes neurogranin affects intracellular signals that are vital for synaptic plasticity and enhanced neural communication.
- Associated diseases and disorders
Neurogranin has a noted involvement in conditions like Alzheimer's disease and cognitive impairment. Changes in neurogranin expression levels are linked to the progression of Alzheimer's where reduced levels may contribute to the disruption of synaptic plasticity. Interactions with amyloid-beta peptides and other proteins such as tau highlight its potential as a biomarker for neurodegenerative diseases. These connections underline neurogranin's significant role in understanding cognitive-related disorders and guiding therapeutic research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Neurogranin antibody [NG22] - BSA and Azide free (Capture) (ab275781), expandable thumbnail](https://content.abcam.com/products/images/neurogranin-antibody-ng22-bsa-and-azide-free-capture-ab275781--sandwich-elisa-img46623.jpg "Sandwich ELISA - Anti-Neurogranin antibody [NG22] - BSA and Azide free (Capture) (ab275781)")
Sandwich ELISA - Anti-Neurogranin antibody [NG22] - BSA and Azide free (Capture) (ab275781)
Standard calibration curve.
Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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